Patents by Inventor Johnathan Charles Sharpe
Johnathan Charles Sharpe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10379029Abstract: A flow cell for particle processing such as particle sorting, may be operatively engaged to a particle processing apparatus. The fluid contact surfaces of the flow cell may be fully enclosed. Further, the flow cell may encapsulate all fluid contact surfaces in the particle processing apparatus. The enclosing or encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The flow cell may employ any suitable technique for processing particles. The flow cell may be disposable and suitable for use in droplet sorting. The flow cell may include an operatively sealed sort chamber having a particle stream focusing region, an orifice, an interrogation zone and a sorting region.Type: GrantFiled: September 18, 2013Date of Patent: August 13, 2019Assignee: CYTONOME/ST, LLCInventors: Johnathan Charles Sharpe, Rudolf Hulspas, Kristopher Scott Buchanan
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Patent number: 10371621Abstract: The present disclosure relates to optical crosstalk reduction in particle processing (e.g., cytometry including flow cytometry using microfluidic based sorters, drop formation based sorters, and/or cell purification) systems and methods in order to improve performance. More particularly, the present disclosure relates to assemblies, systems and methods for minimizing optical crosstalk during the analyzing, sorting, and/or processing (e.g., purifying, measuring, isolating, detecting, monitoring and/or enriching) of particles (e.g., cells, microscopic particles, etc.). The exemplary systems and methods for crosstalk reduction in particle processing systems (e.g., cell purification systems) may be particularly useful in the area of cellular medicine or the like. The systems and methods may be modular and used singly or in combination to optimize cell purification based on the crosstalk environment and specific requirements of the operator and/or system.Type: GrantFiled: May 6, 2016Date of Patent: August 6, 2019Assignee: CYTONOME/ST, LLCInventors: Johnathan Charles Sharpe, Donald Francis Perrault, Jr., Emanuel Tito Mendes Machado, Blair D. Morad
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Patent number: 10371622Abstract: This disclosure relates to a device in the form of a microfluidic chip. In particular, various features are incorporated into the microfluidic chip for aligning and orienting sperm in flow channels, as well as for separating selected subpopulations of sperm.Type: GrantFiled: March 14, 2013Date of Patent: August 6, 2019Assignee: Inguran, LLCInventors: Johnathan Charles Sharpe, Nemanya Sedoglavich, Erin Koksal, Kris Buchanan, Blair Morad
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Patent number: 10349632Abstract: Exemplary embodiments are directed to sensor apparatuses for attachment to an animal that include a housing and a sensor assembly. The housing can be attachable to the animal and includes an internal cavity formed therein. The sensor assembly can be disposed within the internal cavity of the housing. The sensor assembly includes a force sensor and an accelerometer arranged to detect force data and accelerometer data representative of a physiological state of the animal. Exemplary embodiments are also directed to method and sensor systems for detecting a physiological state of an animal.Type: GrantFiled: August 7, 2017Date of Patent: July 16, 2019Assignee: ST Reproductive Technologies, LLCInventors: Johnathan Charles Sharpe, Philip Rowe, Ramakrishnan Vishwanath, Paul Julian Martinsen
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Publication number: 20190154562Abstract: The present disclosure provides improved optical systems for particle processing (e.g., cytometry including microfluidic based sorters, drop sorters, and/or cell purification) systems and methods. More particularly, the present disclosure provides advantageous micro-lens array optical detection assemblies for particle (e.g., cells, microscopic particles, etc.) processing systems and methods (e.g., for analyzing, sorting, processing, purifying, measuring, isolating, detecting, monitoring and/or enriching particles.Type: ApplicationFiled: January 28, 2019Publication date: May 23, 2019Inventors: Johnathan Charles Sharpe, Donald Francis Perrault, JR.
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Patent number: 10274414Abstract: Systems, methods and non-transitory storage medium are disclosed herein for adjusting an output of a particle inspection system representative of a particle characteristic for a particle flowing in a flow-path of a particle processing system. More particularly, the output may be processed and a calibrated output of the particle characteristic generated. In other embodiments, one or more calibration particles are used. Thus, an output of a particle inspection system representative of a particle characteristic for one or more calibration particles flowing in a flow-path of a particle processing system may be compared relative to a standard and an action may be taken based on a result of the comparing the output to the standard.Type: GrantFiled: May 19, 2014Date of Patent: April 30, 2019Assignee: CYTONOME/ST, LLCInventors: Johnathan Charles Sharpe, Emanuel Tito Mendes Machado, Rudolf Hulspas
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Patent number: 10215995Abstract: Large area, low f-number optical systems, and microfluidic systems incorporating such optical systems, are disclosed. Large area, low f-number optical systems may be used to collect light from plurality of micro channels associated with a plurality of flow cytometers. The optical systems may be configured to collect light from a source area having an object lateral length or width within a range of 25 mm and 75 mm, configured to have an f-number within a range of 0.9 to 1.2, and configured to have a working distance within a range of 10 mm to 30 mm.Type: GrantFiled: May 16, 2013Date of Patent: February 26, 2019Assignee: CYTONOME/ST, LLCInventors: Donald Francis Perrault, Jr., Johnathan Charles Sharpe
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Patent number: 10197493Abstract: A microfluidic multiple channel particle analysis system which allows particles from a plurality of particle sources to be independently simultaneously entrained in a corresponding plurality of fluid streams for analysis and sorting into particle subpopulations based upon one or more particle characteristics.Type: GrantFiled: April 10, 2017Date of Patent: February 5, 2019Assignee: CYTONOME/ST, LLCInventors: Johnathan Charles Sharpe, Emanuel Tito Mendes Machado, Blair D. Morad, Rudolf Hulspas, Donald Francis Perrault, Jr.
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Patent number: 10190960Abstract: The present disclosure provides improved optical systems for particle processing (e.g., cytometry including microfluidic based sorters, drop sorters, and/or cell purification) systems and methods. More particularly, the present disclosure provides advantageous micro-lens array optical detection assemblies for particle (e.g., cells, microscopic particles, etc.) processing systems and methods (e.g., for analyzing, sorting, processing, purifying, measuring, isolating, detecting, monitoring and/or enriching particles.Type: GrantFiled: March 13, 2014Date of Patent: January 29, 2019Assignee: CYTONOME/ST, LLCInventors: Johnathan Charles Sharpe, Donald Francis Perrault, Jr.
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Publication number: 20180356846Abstract: The present disclosure provides improved particle processing (e.g., cytometry and/or cell purification) systems and methods that can operate in an autonomous fashion. More particularly, the present disclosure provides for assemblies, systems and methods for analyzing, sorting, and/or processing (e.g., purifying, measuring, isolating, detecting and/or enriching) particles (e.g., cells, microscopic particles, etc.) where human intervention is not required and/or is minimized. The systems, assemblies and methods of the present disclosure advantageously improve run performance of particle processing systems (e.g., cell purification systems, cytometers) by significantly reducing and/or substantially eliminating the burden of operation for human intervention by automating numerous functions, features and/or steps of the disclosed systems and methods.Type: ApplicationFiled: May 7, 2018Publication date: December 13, 2018Inventors: Johnathan Charles Sharpe, Nemanya Sedoglavich, Vladimir Shapiro, Stuart R. Ehrlich, Donald Francis Perrault, JR., Blair D. Morad
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Patent number: 10080348Abstract: Exemplary embodiments are directed to sensor apparatuses for attachment to an animal that include a housing and a sensor assembly. The housing can be attachable to the animal and includes an internal cavity formed therein. The sensor assembly can be disposed within the internal cavity of the housing. The sensor assembly includes a force sensor and an accelerometer arranged to detect force data and accelerometer data representative of a physiological state of the animal. Exemplary embodiments are also directed to method and sensor systems for detecting a physiological state of an animal.Type: GrantFiled: May 20, 2014Date of Patent: September 25, 2018Assignee: ST Reproductive Technologies, LLCInventors: Johnathan Charles Sharpe, Philip Rowe, Ramakrishnan Vishwanath, Paul Julian Martinsen
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Patent number: 9964968Abstract: The present disclosure provides improved particle processing (e.g., cytometry and/or cell purification) systems and methods that can operate in an autonomous fashion. More particularly, the present disclosure provides for assemblies, systems and methods for analyzing, sorting, and/or processing (e.g., purifying, measuring, isolating, detecting and/or enriching) particles (e.g., cells, microscopic particles, etc.) where human intervention is not required and/or is minimized. The systems, assemblies and methods of the present disclosure advantageously improve run performance of particle processing systems (e.g., cell purification systems, cytometers) by significantly reducing and/or substantially eliminating the burden of operation for human intervention by automating numerous functions, features and/or steps of the disclosed systems and methods.Type: GrantFiled: March 13, 2014Date of Patent: May 8, 2018Assignee: CYTONOME/ST, LLCInventors: Johnathan Charles Sharpe, Nemanya Sedoglavich, Vladimir Shapiro, Stuart R. Ehrlich, Donald Francis Perrault, Jr., Blair D. Morad
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Patent number: 9927345Abstract: A nozzle assembly is disclosed. The nozzle assembly may include a nozzle outlet and enclose a nozzle cavity. A sample inlet formed in the nozzle housing may provide fluid communication to an injection tube mounted in an injection stem formed in the nozzle housing. One or more sheath inlet may provide fluid communication with the nozzle cavity and a downstream exit orifice.Type: GrantFiled: July 7, 2016Date of Patent: March 27, 2018Assignee: Inguran, LLCInventors: Kris Buchanan, Johnathan Charles Sharpe, Kenneth Michael Evans
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Publication number: 20180055017Abstract: Exemplary embodiments are directed to sensor apparatuses for attachment to an animal that include a housing and a sensor assembly. The housing can be attachable to the animal and includes an internal cavity formed therein. The sensor assembly can be disposed within the internal cavity of the housing. The sensor assembly includes a force sensor and an accelerometer arranged to detect force data and accelerometer data representative of a physiological state of the animal. Exemplary embodiments are also directed to method and sensor systems for detecting a physiological state of an animal.Type: ApplicationFiled: August 7, 2017Publication date: March 1, 2018Applicant: Accelerenz LimitedInventors: Johnathan Charles Sharpe, Philip Rowe, Ramakrishnan Vishwanath, Paul Julian Martinsen
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Publication number: 20170343467Abstract: A microfluidic multiple channel particle analysis system which allows particles from a plurality of particle sources to be independently simultaneously entrained in a corresponding plurality of fluid streams for analysis and sorting into particle subpopulations based upon one or more particle characteristics.Type: ApplicationFiled: April 10, 2017Publication date: November 30, 2017Inventors: Johnathan Charles Sharpe, Emanuel Tito Mendes Machado, Blair D. Morad, Rudolf Hulspas, Donald Francis Perrault, JR.
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Patent number: 9757726Abstract: This disclosure relates to a system for sorting sperm cells in a microfluidic chip. In particular, various features are incorporated into the system for aligning and orienting sperm in flow channels, as well as, for determining sperm orientation and measuring relative DNA content for analysis and/or sorting.Type: GrantFiled: March 14, 2013Date of Patent: September 12, 2017Assignee: Inguran, LLCInventors: Johnathan Charles Sharpe, Nemanya Sedoglavich, Kris Buchanan, Blair Morad, Donald Francis Perrault, Jr., Erin Koksal
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Patent number: 9618442Abstract: A microfluidic multiple channel particle analysis system which allows particles from a plurality of particle sources to be independently simultaneously entrained in a corresponding plurality of fluid streams for analysis and sorting into particle subpopulations based upon one or more particle characteristics.Type: GrantFiled: June 15, 2015Date of Patent: April 11, 2017Assignee: CYTONOME/ST, LLCInventors: Johnathan Charles Sharpe, Emanuel Tito Mendes Machado, Blair D. Morad, Rudolf Hulspas, Donald Francis Perrault, Jr.
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Publication number: 20160327469Abstract: The present disclosure relates to optical crosstalk reduction in particle processing (e.g., cytometry including flow cytometry using microfluidic based sorters, drop formation based sorters, and/or cell purification) systems and methods in order to improve performance. More particularly, the present disclosure relates to assemblies, systems and methods for minimizing optical crosstalk during the analyzing, sorting, and/or processing (e.g., purifying, measuring, isolating, detecting, monitoring and/or enriching) of particles (e.g., cells, microscopic particles, etc.). The exemplary systems and methods for crosstalk reduction in particle processing systems (e.g., cell purification systems) may be particularly useful in the area of cellular medicine or the like. The systems and methods may be modular and used singly or in combination to optimize cell purification based on the crosstalk environment and specific requirements of the operator and/or system.Type: ApplicationFiled: May 6, 2016Publication date: November 10, 2016Inventors: Johnathan Charles Sharpe, Donald Francis Perrault, JR., Emanuel Tito Mendes Machado, Blair D. Morad
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Publication number: 20160320287Abstract: A nozzle assembly is disclosed. The nozzle assembly may include a nozzle outlet and enclose a nozzle cavity. A sample inlet formed in the nozzle housing may provide fluid communication to an injection tube mounted in an injection stem formed in the nozzle housing. One or more sheath inlet may provide fluid communication with the nozzle cavity and a downstream exit orifice.Type: ApplicationFiled: July 7, 2016Publication date: November 3, 2016Applicant: Inguran, LLCInventors: Kris Buchanan, Johnathan Charles Sharpe, Kenneth Michael Evans
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Patent number: D815754Type: GrantFiled: May 16, 2014Date of Patent: April 17, 2018Assignee: CYTONOME/ST, LLCInventors: Michael I. Morkos, Johnathan Charles Sharpe, Jeffrey T. Samson, James V. Osburn, Martin Joe Costello