Abstract: Provided herein is a centrifuge chamber, useful in the separation and concentration of particles from cell suspensions. In particular, the centrifuge chamber described herein can be advantageously used to separate and concentrate desired cell populations from cell suspensions, e.g., from biological samples or tissues. Also provided are methods of separating and concentrating desired particles (e.g., desired cell populations), from suspensions (e.g., cell suspensions) using the centrifuge chamber described herein. Kits including a centrifuge chamber as described herein, and optionally other components (e.g., a centrifuge), are also provided.