Abstract: Disclosed are methods and systems to detect a class of non-variola Orthopoxviruses, and, more particularly, monkeypox virus. In certain embodiments, the method may comprise obtaining a sample from the subject; and detecting a nucleic acid sequence specific to the non-variola Orthopoxvirus. In some embodiments, the method may further isolation of the non-variola Orthopoxvirus from a solid substrate such as a tissue swab and further purification of viral nucleic acid using silica beads. The sample may in some cases be self-collected by a subject. The disclosed methods and systems, including kits for self-collection, may be used for a high throughput assay allowing for screening of hundreds of samples per day.

Abstract: Holographic volume gratings in waveguide cells can be recorded using many different methods and systems in accordance with various embodiments of the invention. One embodiment includes a holographic recording system including at least one laser source configured to emit recording beams and a movable platform configured to move between a first position and a second position, wherein when the movable platform is in the first position, the at least one laser source is configured to emit a first set of one or more recording beams toward a first set of one or more stations and when the movable platform is in the second position, the at least one laser source is configured to emit a second set of one or more recording beams toward a second set of one or more stations.

Abstract: Disclosed herein are methods and systems comprising obtaining nucleic acid from a sample that was obtained from a subject; capturing and amplifying a target molecule in the nucleic acid using a molecular inversion probe under hybridization conditions; ligating an adapter to create a circular molecule; sequencing the circular molecule to obtain sequence reads; generating a sequencing file comprising the sequence reads of each molecule and a position of each sequence read in a reference genome of a virus; and generating a reporting file for the subject comprising a predicted lineage of the virus in the sample.

Abstract: There is disclosed system for oxygenating a patient in relation to anaesthesia using high flow gas delivery. The system has a flow source, and a controller for determining oxygenation requirements of the patient before or during anaesthesia. A method of oxygenating a patient in relation to anaesthesia using high flow gas delivery is also disclosed. The method determines oxygenation requirements of the patient before or during anaesthesia.

Abstract: Disclosed are methods and systems and computer program products for determining DPB1 expression without the need for rs9277534 (3? UTR) sequence data. Such methods are useful in assessing HLA sequencing submitted to or already in donor databases for matches that will reduce the risk of hematopoietic transplant rejection (i.e., graft vs. host disease).

Abstract: Disclosed are methods and systems for the detection of variants of the SARS-CoV-2 virus that cause COVID-19. For example, disclosed are methods for identifying and/or tracking variants of SARS-CoV-2 comprising: (a) identifying a sample from a subject as positive for SARS-CoV-2 nucleic acid and/or antibodies to SARS-CoV-2; (b) generating a sample-specific SARS-CoV-2 nucleic acid from the sample; (c) performing nucleic acid sequencing on the sample-specific SARS-CoV-2 nucleic acid; and(d) determining whether the nucleic acid sequence comprises a SARS-CoV-2 variant sequence. Also disclosed are systems for performing any portion of the disclosed methods and computer-program products tangibly embodied in a non-transitory machine-readable storage medium, including instructions configured to perform any of the steps of the disclosed methods or run any portion of the disclosed systems.

Abstract: A communication system and method of operating the same that includes, in one embodiment, a transmit filter including a transmit filter capacitor, and a receive filter coupled to the transmit filter. The communication system also includes a switch, coupled to an auxiliary winding of a transformer and to the transmit filter capacitor, configured to alter an impedance of the transmit filter during a receive mode of operation of the communication system. In another embodiment, a transmitter is configured to receive an input data stream, and produce a control signal for a switch-mode power amplifier employing a predetermined data sequence selected by the input data stream thereto.

Abstract: A communication system and method of operating the same that includes, in one embodiment, a transmit filter including a transmit filter capacitor, and a receive filter coupled to the transmit filter. The communication system also includes a switch, coupled to an auxiliary winding of a transformer and to the transmit filter capacitor, configured to alter an impedance of the transmit filter during a receive mode of operation of the communication system. In another embodiment, a transmitter is configured to receive an input data stream, and produce a control signal for a switch-mode power amplifier employing a predetermined data sequence selected by the input data stream thereto.

Abstract: A controllable electrical switch having a power system and switching device for connecting a source voltage to a load. The power system includes a switching regulator to extract energy directly off the non filtered full wave rectified input voltage to minimize the average magnitude of the current when the switching device is in the off-state. For on-state power the controllable electrical switch may include a transformer connected in series with the switching device. The controllable electrical switch and power system may be used to replace a standard two- wire or three-wire wall switch, and may be used over a wide voltage and load power range in a low cost, small package with low power dissipation.