Abstract: A method of charge stripping analyte ions is disclosed. The method comprises reacting the analyte ions with reagent ions or charged particles; and then urging the reacted analyte ions through a neutral, inert gas such that said analyte ions interact or collide with the gas molecules in a manner that reduces the charge state of the reacted analyte ions, thereby forming product ions of reduced charge state. The combination of reacting the analyte ions and then urging the reacted ions through the gas results in a charge reduction that is greater than that which would be caused by either of the individual steps of reacting the ions or urging the ions through the gas.

Abstract: A method of charge stripping analyte ions is disclosed. The method comprises reacting the analyte ions with reagent ions or charged particles; and then urging the reacted analyte ions through a neutral, inert gas such that said analyte ions interact or collide with the gas molecules in a manner that reduces the charge state of the reacted analyte ions, thereby forming product ions of reduced charge state. The combination of reacting the analyte ions and then urging the reacted ions through the gas results in a charge reduction that is greater than that which would be caused by either of the individual steps of reacting the ions or urging the ions through the gas.

Abstract: A method for the detection and quantitation of analytes of interest and variants of the analyte of interest comprising the steps of: (i) providing a sample containing an analyte of interest; (ii) spiking sample with known amount of calibrant; (iii) performing an LCMS or LCMSMS analysis on the spiked sample to produce a data set; (iv) determining from said data set the relative quantity of analyte of interest to calibrant; (v) calculating the absolute quantity of the analyte of interest from said relative quantity of analyte of interest and said known amount of calibrant; (vi) searching for one or more previously identified candidate sequences for one or more known variants denoted by one or more specific peaks to identify the presence of said one or more known variants within the sample; (vii) determining the relative quantity or amount of said one or more variants to said analyte of interest; and (viii) calculating the absolute quantity or amount of any of said one or more variants present in the sample.

Abstract: A mass spectrometry electron transfer dissociation reagent comprising an unsaturated compound having a Frank Condon factor between 0.1 and 1.0 and an electron affinity having a positive value between 0.1 to 150 kJ/mol.