Patents by Inventor Jonathan Rothberg
Jonathan Rothberg has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8450781Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.Type: GrantFiled: September 14, 2012Date of Patent: May 28, 2013Assignee: Life Technologies CorporationInventors: Jonathan Rothberg, Wolfgang Hinz, Kim Johnson, James Bustillo
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Patent number: 8445945Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.Type: GrantFiled: July 20, 2012Date of Patent: May 21, 2013Assignee: Life Technologies CorporationInventors: Jonathan Rothberg, Wolfgang Hinz, Kim Johnson, James Bustillo
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Patent number: 8441044Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.Type: GrantFiled: July 20, 2012Date of Patent: May 14, 2013Assignee: Life Technologies CorporationInventors: Jonathan Rothberg, Wolfgang Hinz, Kim Johnson, James Bustillo
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Patent number: 8435395Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.Type: GrantFiled: September 14, 2012Date of Patent: May 7, 2013Assignee: Life Technologies CorporationInventors: Jonathan Rothberg, Wolfgang Hinz, Kim Johnson, James Bustillo
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Patent number: 8415716Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.Type: GrantFiled: July 23, 2012Date of Patent: April 9, 2013Assignee: Life Technologies CorporationInventors: Jonathan Rothberg, Wolfgang Hinz, Kim Johnson, James Bustillo
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Publication number: 20120264632Abstract: The claimed invention provides for new sample preparation methods enabling direct sequencing of PCR products using pyrophosphate sequencing techniques. The PCR products may be specific regions of a genome. The techniques provided in this disclosure allows for SNP (single nucleotide polymorphism) detection, classification, and assessment of individual allelic polymorphisms in one individual or a population of individuals. The results may be used for diagnostic and treatment of patients as well as assessment of viral and bacterial population identification.Type: ApplicationFiled: April 10, 2012Publication date: October 18, 2012Inventors: John Harris Leamon, William Lun Lee, Jan Fredrik Simons, Brian Desany, Michael Todd Ronan, James Drake, Kenton Lohman, Michael Egholm, Jonathan Rothberg
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Publication number: 20120247977Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.Type: ApplicationFiled: July 28, 2011Publication date: October 4, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Jonathan ROTHBERG, Wolfgang HINZ, Kim JOHNSON, James BUSTILLO, John LEAMON, Jonathan SCHULTZ
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Publication number: 20120202276Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.Type: ApplicationFiled: February 28, 2011Publication date: August 9, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: John Davidson, Wolfgang Hinz, Jonathan Rothberg, Richard Whitaker
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Publication number: 20120094871Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.Type: ApplicationFiled: February 15, 2011Publication date: April 19, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Wolfgang HINZ, John Leamon, David Light, Jonathan Rothberg
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Publication number: 20120065093Abstract: Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.Type: ApplicationFiled: September 9, 2011Publication date: March 15, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Jonathan Rothberg, Wolfgang Hinz
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Publication number: 20120045844Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.Type: ApplicationFiled: September 26, 2011Publication date: February 23, 2012Applicant: Life Technologies CorporationInventors: Jonathan ROTHBERG, Wolfgang Hinz, Kim Johnson, James Bustillo
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Publication number: 20110318748Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.Type: ApplicationFiled: February 25, 2011Publication date: December 29, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: John Davidson, Wolfgang Hinz, Jonathan Rothberg, Richard Whitaker
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Publication number: 20110201508Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.Type: ApplicationFiled: February 17, 2011Publication date: August 18, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: WOLFGANG HINZ, JOHN LEAMON, DAVID LIGHT, JONATHAN ROTHBERG
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Publication number: 20110201523Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.Type: ApplicationFiled: February 15, 2011Publication date: August 18, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Wolfgang HINZ, John Leamon, David Light, Jonathan Rothberg
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Publication number: 20110201506Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.Type: ApplicationFiled: February 17, 2011Publication date: August 18, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Wolfgang HINZ, John Leamon, David Light, Jonathan Rothberg
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Publication number: 20110195253Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.Type: ApplicationFiled: February 14, 2011Publication date: August 11, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Wolfgang HINZ, John LEAMON, David LIGHT, Jonathan ROTHBERG
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Publication number: 20110195252Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.Type: ApplicationFiled: February 14, 2011Publication date: August 11, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Wolfgang HINZ, John Leamon, David Light, Jonathan Rothberg
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Publication number: 20110195459Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.Type: ApplicationFiled: February 17, 2011Publication date: August 11, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Wolfgang HINZ, John Leamon, David Light, Jonathan Rothberg
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Publication number: 20100300895Abstract: The invention is directed to apparatus and methods for delivering multiple reagents to, and monitoring, a plurality of analytical reactions carried out on a large-scale array of electronic sensors underminimal noise conditions. In one aspect, the invention provides method of improving signal-to-noise ratios of output signals from the electronic sensors sensing analytes or reaction byproducts by subtracting an average of output signals measured from neighboring sensors where analyte or reaction byproducts are absent. In other aspects, the invention provides an array of electronic sensors integrated with a microwell array for confining analytes and/or particles for analytical reactions and a method for identifying microwells containing analytes and/or particles by passing a sensor-active reagent over the array and correlating sensor response times to the presence or absence of analytes or particles.Type: ApplicationFiled: May 24, 2010Publication date: December 2, 2010Applicant: ION TORRENT SYSTEMS, INC.Inventors: John Nobile, Thomas Roth, Todd Rearick, Jonathan Schultz, Jonathan Rothberg, David Marran
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Publication number: 20080014589Abstract: The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.Type: ApplicationFiled: May 11, 2007Publication date: January 17, 2008Inventors: Darren Link, Michael Weiner, David Marran, Jonathan Rothberg