Abstract: Disclosed are particles which are introduced into target cells and suppress the expression of specific genes, and a method of manufacturing such particles. More particularly, the present invention relates to DNA-RNA hybrid particles that comprise a DNA strand and an RNA strand that binds to the DNA strand through partial complementary base pairing, in which the DNA strand comprises an aptamer sequence that is able to bind to a target protein produced in a target cell, and the RNA strand comprises an siRNA sequence that binds to a target RNA in the target cell to suppress protein expression from the target RNA. Such hybrid particles are capable of effectively delivering an siRNA therapeutic agent into target cells for the treatment of disease, and have resistance against digestion by in vivo nucleases, DNase and RNase, owing to complementary binding formed between DNA and RNA strands. Also, the present invention relates to a method of manufacturing such DNA-RNA hybrid particles.

Abstract: Provided are a self-assembled polyribonucleotide-protein complex including a polyribonucleotide including a plurality of first repeating units having a single guide RNA (sgRNA) region and a small interfering RNA (siRNA) region; and one or more nuclease proteins binding to the sgRNA region, and use thereof.

Abstract: The present disclosure relates to a battery aging state calculation method and device, and more particularly, to a battery aging state calculation method and device for calculating a (i.e., State of Health (SOH)) according to each execution operation and calculating a final battery SOH based on charge/discharge execution counts to improve the accuracy of the battery SOH.

Abstract: A battery power table setting method for using battery power efficiently and preventing battery overcharge includes: a first table generation operation for generating a first table by measuring a maximum charging power that prevents an output of a battery from being more than a maximum allowable voltage when the battery having a predetermined SOC value is charged at a predetermined temperature for a predetermined time; a second table generation operation for generating a second table by measuring an SOC value at the time point that the battery reaches the maximum allowable voltage when the battery is charged with a predetermined power value at a predetermined temperature; a third table generation operation for generating a third table by calculating a second maximum charging power according to a predetermined temperature and a predetermined SOC value based on the second table; and a derating table generation operation for generating a derating table.

Abstract: A package for a chemical sensor including an encapsulation and a pressure balancing structure is disclosed. The encapsulation encapsulates a chemical sensor and has a hole for exposing a chemical sensitive part of the chemical sensor. The pressure balancing structure balances pressure applied to the chemical sensor at the chemical sensitive part.

Abstract: The system heats glycol water using steam generated by a boiler and heating LNG using the glycol water, thereby increasing efficiently the LNG to temperature required for an engine. In addition, the system senses LNG flowing to a glycol tank using a pressure sensor, etc. when the LNG flows to the glycol tank due to pressure difference between a fuel supplying line and a glycol circulation line generated according as a heat exchanger is broken down, and outputs the flowed LNG to the outside. As a result, the glycol circulation line may be returned to original state and stability of the system may be enhanced.

Abstract: Disclosed are particles which are introduced into target cells and suppress the expression of specific genes, and a method of manufacturing such particles. More particularly, the present invention relates to DNA-RNA hybrid particles that comprise a DNA strand and an RNA strand that binds to the DNA strand through partial complementary base pairing, in which the DNA strand comprises an aptamer sequence that is able to bind to a target protein produced in a target cell, and the RNA strand comprises an siRNA sequence that binds to a target RNA in the target cell to suppress protein expression from the target RNA. Such hybrid particles are capable of effectively delivering an siRNA therapeutic agent into target cells for the treatment of disease, and have resistance against digestion by in vivo nucleases, DNase and RNase, owing to complementary binding formed between DNA and RNA strands. Also, the present invention relates to a method of manufacturing such DNA-RNA hybrid particles.

Abstract: The present invention provides, among other things, a particle which includes a core comprised of self-assembled one or more nucleic acid molecules, the core being characterized by an ability to adopt at least two configurations: a first configuration having a first greatest dimension greater than 2 ?m and; a second configuration having a second greatest dimension less than 500 nm, wherein addition of a film coating converts the core from its first configuration to its second configuration. Methods of making and using of provided particles are also disclosed.

Abstract: Disclosed are nanoparticles that are introduced into cells and express a specific protein and a manufacturing method thereof. More particularly, the present invention relates to mRNA nanoparticles, which increase the expression of a specific protein capable of stimulating the cellular immune system to induce cellular immune responses and are thus applicable to treat a variety of diseases, do not require passage across the nuclear envelope because a desired gene is delivered not as plasmid DNA itself but in the form of mRNA, thus improving the efficiency of protein expression, and the nanoparticles are generated through a one-step process with a relatively small amount of plasmid DNA via rolling circle transcription (RCT), thereby providing a simple and economical process for gene delivery. The present invention is also concerned with such mRNA nanoparticles.

Abstract: Disclosed are a method of manufacturing an RNA membrane and an RNA membrane manufactured thereby, wherein the RNA membrane can be produced at lower cost, in which the RNA membrane is composed exclusively of RNA, and thus has no toxicity in vivo, is controllable, and can be effectively applied to bio-organs such as the pericardium, as well as the production of peptides or proteins, and particularly, long linear RNA strands, which have not yet formed particles, are concentrated on the surface of a tube by inducing an evaporation process to thus activate the bonding of base pairs thereof, and the roughness and thickness of the RNA membrane can be controlled by changing the manufacturing conditions.

Abstract: A package for a chemical sensor including an encapsulation and a pressure balancing structure is disclosed. The encapsulation encapsulates a chemical sensor and has a hole for exposing a chemical sensitive part of the chemical sensor. The pressure balancing structure balances pressure applied to the chemical sensor at the chemical sensitive part.

Abstract: Disclosed are a method of manufacturing an RNA membrane and an RNA membrane manufactured thereby, wherein the RNA membrane can be produced at lower cost, in which the RNA membrane is composed exclusively of RNA, and thus has no toxicity in vivo, is controllable, and can be effectively applied to bio-organs such as the pericardium, as well as the production of peptides or proteins, and particularly, long linear RNA strands, which have not yet formed particles, are concentrated on the surface of a tube by inducing an evaporation process to thus activate the bonding of base pairs thereof, and the roughness and thickness of the RNA membrane can be controlled by changing the manufacturing conditions.

Abstract: A package for a chemical sensor including an encapsulation and a pressure balancing structure is disclosed. The encapsulation encapsulates a chemical sensor and has a hole for exposing a chemical sensitive part of the chemical sensor. The pressure balancing structure balances pressure applied to the chemical sensor at the chemical sensitive part.

Abstract: The present invention provides, among other things, a particle which includes a core comprised of self-assembled one or more nucleic acid molecules, the core being characterized by an ability to adopt at least two configurations: a first configuration having a first greatest dimension greater than 2 ?m and; a second configuration having a second greatest dimension less than 500 nm, wherein addition of a film coating converts the core from its first configuration to its second configuration. Methods of making and using of provided particles are also disclosed.

Abstract: A package for a chemical sensor including an encapsulation and a pressure balancing structure is disclosed. The encapsulation encapsulates a chemical sensor and has a hole for exposing a chemical sensitive part of the chemical sensor. The pressure balancing structure balances pressure applied to the chemical sensor at the chemical sensitive part.

Abstract: The present invention provides, among other things, a particle which includes a core comprised of self-assembled one or more nucleic acid molecules, the core being characterized by an ability to adopt at least two configurations: a first configuration having a first greatest dimension greater than 2 ?m and; a second configuration having a second greatest dimension less than 500 nm, wherein addition of a film coating converts the core from its first configuration to its second configuration. Methods of making and using of provided particles are also disclosed.

Abstract: A system for supplying LNG fuel is disclosed. The system includes a fuel supplying line connected from an LNG storage tank to a high pressure engine and a low pressure engine, respectively, a pump formed on the fuel supplying line, and configured to pressurize LNG outputted from the LNG storage tank to high pressure, a first heat exchanger formed on the fuel supplying line between the high pressure engine and the pump, and configured to heat the LNG supplied from the pump, a second heat exchanger formed on the fuel supplying line between the low pressure engine and the LNG storage tank, and configured to evaporate the LNG in a liquid state supplied from the LNG storage tank and a heat source supplying line configured to supply heat to the LNG by supplying glycol water to the first heat exchanger and the second heat exchanger.

Abstract: The system heats glycol water using steam generated by a boiler and heating LNG using the glycol water, thereby increasing efficiently the LNG to temperature required for an engine. In addition, the system senses LNG flowing to a glycol tank using a pressure sensor, etc. when the LNG flows to the glycol tank due to pressure difference between a fuel supplying line and a glycol circulation line generated according as a heat exchanger is broken down, and outputs the flowed LNG to the outside. As a result, the glycol circulation line may be returned to original state and stability of the system may be enhanced.

Abstract: Methods and compositions are provided for producing nucleic acid-based compositions. Methods include enzyme catalyzed or nucleic acid polymer conjugation. Compositions include nucleic acid-containing hydrogels which can be elongated via rolling circle amplification. The hydrogels can encapsulate bioactive agents for drug delivery.

Abstract: Compositions and methods are provided for constructing multi-functional nucleic acid nano-structures. Nano-structures are provided incorporating a built-in modularity, including nucleic acid modules. Modules contain moieties including detectible labels, nanoparticles, reactive moieties and other functional groups. Nano-structures can be used for delivery of target compounds, as well as identification of target nucleic acid molecules.