Abstract: The present invention provides a universally usable knockout cassette that contains a selectable marker gene and is characterized by the specific design of the restriction cleavage sites. A method for preparing the knockout cassette is also provided as are methods of using the knockout cassette.

Abstract: Described is a mine clearance system (10) comprising a mine clearance vehicle (12), at the front side of which there is a frontal attachment with a milling roller (26), the frontal attachment being pivotable about a horizontal axis (40). A tail attachment forming a mine-seeking apparatus can be provided on the mine clearance vehicle (12), or a specific mine-seeking vehicle (14) with such a tail attachment can be arranged after the mine clearance vehicle (12). That tail attachment or the mine-seeking vehicle (14) has a second milling roller (44) which is disposed pivotably about a horizontal axis in order to be able to adjust as desired the height or the depth of penetration into the ground of the second milling roller. A conveyor and sifting device (58) is arranged after the second milling roller (44).