Abstract: Methods are disclosed herein for efficiently generating human induced pluripotent stem cells (iPSC) containing a nucleic acid including a doxycycline promoter operably linked to a nucleic acid encoding Cas9. These methods include transfecting a human somatic cell with a nucleic acid molecule comprising a doxycycline promoter operably linked to a nucleic acid encoding a Cas9, and constitutive promoter operably linked to a tetracycline responsive element and inducing the somatic cell to form an iPSC, thereby producing an iPSC that can undergo CRISPR/Cas9-mediated recombination at a high efficiency. The human iPSC, or a cell differentiated therefrom, is cultured in the presence of doxycycline to induce expression of the Cas9. These cells can then be used to target in any gene of interest by introducing nucleic acids encoding sgRNAs. Induced pluripotent stem cells produced by these methods are also disclosed.

Abstract: Methods are disclosed herein for producing human hepatocytes from human induced pluripotent stem cells. Also provided are transgenic rats for the expansion of human hepatocytes, such as those produced using the methods disclosed herein.

Abstract: Disclosed are compositions and methods for treating a liver disease in a subject by increasing transport or retention of HNF4?, a transcriptional factor, into a nucleus of a hepatocyte in the subject. In some embodiments, the method comprises upregulating expression or function of one or more transcription factors selected from the group consisting of PROX1, NR5A2, NROB2, MTF1, SREBP1, EP300, and POM121C, and functional fragments thereof, and/or downregulating expression or function of one or more transcription factors DNAJB 1/F1SP40, ATF6, ATF4, and PERK, and functional fragments thereof.

Abstract: Methods are disclosed herein for producing human hepatocytes from human induced pluripotent stem cells. Also provided are transgenic rats for the expansion of human hepatocytes, such as those produced using the methods disclosed herein.

Abstract: Methods are disclosed herein for efficiently generating human induced pluripotent stem cells (iPSC) containing a nucleic acid including a doxycycline promoter operably linked to a nucleic acid encoding Cas9. These methods include transfecting a human somatic cell with a nucleic acid molecule comprising a doxycycline promoter operably linked to a nucleic acid encoding a Cas9, and constitutive promoter operably linked to a tetracycline responsive element and inducing the somatic cell to form an iPSC, thereby producing an iPSC that can undergo CRISPR/Cas9-mediated recombination at a high efficiency. The human iPSC, or a cell differentiated therefrom, is cultured in the presence of doxycycline to induce expression of the Cas9. These cells can then be used to target in any gene of interest by introducing nucleic acids encoding sgRNAs. Induced pluripotent stem cells produced by these methods are also disclosed.

Abstract: Provided herein are methods or making and using whole or partial organ ECM structures comprising an anticoagulant. Also provided are organ structures prepared according to those methods.

Abstract: A mobile wireless system (10) includes a transmitter such as a satellite (18) that broadcasts wireless signals such as boundaries for specific areas to a mobile receiving device (22). Mobile receiving device (22) may include an antenna (26) and a mobile receiving device (28). A locating receiver (50) is used to generate locating signals so that a determination may be made to the location of the mobile receiving device. When the location is outside an area of interest the area of interest may be extended to provide services to the mobile device.

Abstract: A system and method for playing back digital satellite audio and video signals includes storing a file on a storage device associated with the satellite receiver 54. The file is communicated to a file playback device such as a mobile receiver 28 from the storage device. The file is played back using the mobile receiving device 28.

Abstract: A system and method to provide a means of communication, command and control between a mobile antenna and a satellite receiver that allows the receiver to send tuning information to the antenna and the antenna to provide feedback to the receiver when a signal has been acquired. The antenna and the receiver can share the appropriate states and status such as diagnostics, test, GPS coordinates, etc.

Abstract: A receiving unit 28 includes a tuner circuit board 104 that receives a satellite signal. The tuner circuit board 104 demodulates and decodes the satellite signal to form a second signal. The second signal is provided to a processor board 112 that is separated from the tuner and formats the second signal to form an audio signal and a video signal. An integrated bus 100 couples the tuner circuit board 104 and the processor circuit board 112. The integrated bus 100 comprises the second signal, the audio signal and the video signal.

Abstract: The invention relates to a fixing device (8) for a expansion valve (4) of a motor vehicle air-conditioning system (1), said expansion valve (4) being connected to two lines (5, 6). The two lines (5, 6) are positioned in relation to each other by means of a line positioning element (7) which is embodied as a sheet metal stamped part, said line positioning element comprising two slits which are used to receive the lines. The expansion valve (4) can be arranged on the housing (2), with the aid of the line positioning element (7), and can be fixed in an at least rotationally fixed manner, wherein one part of the motor vehicle air-conditioning system (1) is arranged.

Abstract: A system and method for providing conditional access authorizations in a satellite-receiving system includes generating a conditional access authorization signal and an expiration signal at a head-end 20, receiving the conditional access authorization signal at a fixed satellite receiver 54 or at a mobile satellite receiver 28. When the mobile satellite receiver operates in a predetermined time window before the expiration time, which is determined from the expiration signal, a warning 434 on a screen display 432 is generated.

Abstract: A system and method to provide a means of communication, command and control between a mobile antenna and a satellite receiver that allows the receiver to send tuning information to the antenna and the antenna to provide feedback to the receiver when a signal has been acquired. The antenna and the receiver can share the appropriate states and stats such as diagnostics, test, GPS coordinates, etc.

Abstract: A system and method to provide a means of communication, command and control between a mobile antenna and a satellite receiver that allows the receiver to send tuning information to the antenna and the antenna to provide feedback to the receiver when a signal has been acquired. The antenna and the receiver can share the appropriate states and stats such as diagnostics, test, GPS coordinates, etc.

Abstract: A method, system and computer program product for efficient storage of data for use by an application, including a set top box. The set top box including a physical memory, a bulk storage device, and a memory management unit (MMU) coupled between the application and the physical memory and the bulk storage device. The physical memory and the bulk storage device are configured to store the data. The MMU is configured to translate a virtual address provided by the application to a physical address used by one of the physical memory and the bulk storage device.

Abstract: A method, system and computer program product for efficient storage of data for use by an application, including a set top box. The set top box including a physical memory, a bulk storage device, and a memory management unit (MMU) coupled between the application and the physical memory and the bulk storage device. The physical memory and the bulk storage device are configured to store the data. The MMU is configured to translate a virtual address provided by the application to a physical address used by one of the physical memory and the bulk storage device.

Abstract: A system and method for providing conditional access authorizations in a satellite-receiving system includes generating a conditional access authorization signal and an expiration signal at a head-end 20, receiving the conditional access authorization signal at a fixed satellite receiver 54 or at a mobile satellite receiver 28. When the mobile satellite receiver operates in a predetermined time window before the expiration time, which is determined from the expiration signal, a warning 434 on a screen display 432 is generated.

Abstract: The invention relates to a fixing device (8) for a expansion valve (4) of a motor vehicle air-conditioning system (1), said expansion valve (4) being connected to two lines (5, 6). The two lines (5, 6) are positioned in relation to each other by means of a line positioning element (7) which is embodied as a sheet metal stamped part, said line positioning element comprising two slits which are used to receive the lines. The expansion valve (4) can be arranged on the housing (2), with the aid of the line positioning element (7), and can be fixed in an at least rotationally fixed manner, wherein one part of the motor vehicle air-conditioning system (1) is arranged.

Abstract: A system and method for playing back digital satellite audio and video signals includes storing a file on a storage device associated with the satellite receiver 54. The file is communicated to a file playback device such as a mobile receiver 28 from the storage device. The file is played back using the mobile receiving device 28.

Abstract: A system and method for providing call-back in a mobile satellite system 10 includes generating a call-back signal at a mobile satellite receiving device 28 and wirelessly communicating the call-back signal to a head end 20. Various methods, including wireless, cellular and WiFi networks may be used to communicate the signal.