Abstract: Methods are provided herein for assembling at least two nucleic acids using a sequence specific nuclease agent (e.g., a gRNA-Cas complex) to create end sequences having complementarity and subsequently assembling the overlapping complementary sequences. The nuclease agent (e.g., a gRNA-Cas complex) can create double strand breaks in dsDNA in order to create overlapping end sequences or can create nicks on each strand to produce complementary overhanging end sequences. Assembly using the method described herein can assemble any nucleic acids having overlapping sequences or can use a joiner oligo to assemble sequences without complementary ends.

Abstract: The invention is directed to a method of livestock breeding, meat production and marketing, histology, and genetics using a biopsy sample from a non-meat location of the livestock to provide information about the meat quality of the livestock.

Abstract: The invention is a combination solar panel and planar array antenna for a satellite. In detail, the invention includes a generally flat solar panel having external and internal surfaces, the external surface having solar energy collecting devices thereon. A generally flat planar antenna having external and internal surfaces is coupled to the flat solar panel, with the external surface for receiving and transmitting electromagnetic energy. The planar array is coupled to the solar panel such that the interior surfaces of the solar array and the planar antenna face each other. The interior surfaces of the solar array and the planar antenna are high emissive thermal surfaces. The external surface of the planar array is a low absorptivity and high emissivity surface.