Abstract: The invention relates to a method for the design and/or the preparation of a polynucleotide capture molecule for detecting an amplicon having one strand serving as target to be detected and/or quantified after hybridization on said capture molecule, comprising the steps of: (a) selecting a primer pair defining the amplicon; (b) selecting a specific sequence of 10 to 100 nucleotides within the amplicon, such that said specific sequence defines two non-complementary ends of the amplicon; (c) defining the capture molecule having a capture portion that is complementary to the specific sequence selected in step b), and a spacer portion comprising at least 20 nucleotides; and (d) identifying among the two non-complementary ends of the amplicon a spacer end and a non-spacer end, respectively, such that the spacer end is non-complementary to the spacer portion of the capture molecule, and said spacer end exceeds said non-spacer end by at least 50 bases.

Abstract: The present invention provides an electronic transducer (10) and a method for detecting and/or characterizing target materials or physico-chemical stimuli in an external medium (8) using the electronic transducer (10). The electronic transducer (10) comprises a sensing element (3) featuring a variable conductance when exposed to a stimulus from the external medium and a first and second electrode (5a, 5b) spaced apart on or in a sensing material surface of a substrate, the sensing element being provided in or on the substrate and being located between the first and the second electrodes (5a, 5b) forming a pair of sensing electrodes for sensing a change in conductance of the sensing element (3) in a direction substantially parallel to the sensing material surface, at least one of the sensing electrodes (5a, 5b) being electrically insulated from the sensing element (3) by a dielectric layer (4), so as to be capacitively coupled to the sensing element (3).

Abstract: A method for identifying a genetically modified plant by identification and/or quantification of different, multiple nucleotide sequence elements corresponding to at least a portion of an exogenous nucleotide sequence integrated into the genome of the genetically modified plant. The method involves amplifying a nucleic acid sequence element into target nucleic acid sequences, contacting the target nucleic acid sequences with single stranded capture nucleotide sequences, detecting binding of the target nucleotide sequences, constructing a genetic map, and identifying the genetically modified plant.

Abstract: A reaction chamber (1) for real-time PCR configured for containing a PCR solution (3), said chamber having a capture molecule (4) immobilized on the chamber inner surface, said surface being configured to be in intermittent contact with the PCR solution (3), and a window (7) permitting detection of a signal from said inner surface by a reader (6) located outside the chamber, and wherein the immobilized capture molecule (4) is not labeled.

Abstract: The present invention proposes a simple and effective lid being part of a device having multiwells for performing simultaneous amplifications by PCR and detections of multiple target molecules on unlabeled capture molecules immobilized on the lid.

Abstract: The invention relates a method for detecting and/or quantifying different target molecules being bound at different locations (spots) of a surface of an optically transparent solid support having a refractive index n1 by specific interaction with capture molecules being immobilized on the solid support surface, said solid support being in contact with a solution having refractive index n2, whereby n1>n2 and wherein the target molecules are in contact with the capture molecules, said method comprising the steps of: a) illuminating homogeneously the surface of the support on which the target molecules are bound, thereby causing homogeneous excitation of the target molecule; b) detecting light emitted from the target molecules in at least four different locations (spots) in the following way: collecting the emitted light through a side of said support which is inclined relative to the surface of the support on which the target molecules are bound, focusing the emitted light by a lens on a detector surface

Abstract: The present invention relates to a method and apparatus for monitoring on a micro-array a PCR amplification of a nucleotide molecule being present in a solution.

Abstract: A method for obtaining an activation profile of a biological sample by disposing onto a solid support in a pre-determined spatial arrangement a subset of capture molecules able to interact with one or more activated transcription factor(s) present in the biological sample, contacting the biological sample upon the solid support under conditions allowing their interaction, monitoring signals resulting from their interaction, and providing a cellular activation profile from the detected signals.

Abstract: The present invention is related to a method, kit and computer program for detecting the presence in a sample of an unspecified Genetically Modified Organism (GMO).

Abstract: The present invention discloses an improved cDNA microarray, which employs spacers of random sequence and a length of the spacers of at least 50 to 80 nucleotides. The inventive cDNA microarray may be employed for example in fields like the determination of gene expression, DNA sequencing, fingerprinting or mapping. In addition, a method for the preparation of the cDNA microarray, the use of spacer molecules with random sequence and a kit are specified.

Abstract: A method quantifies a target compound selected from the group consisting of a polynucleotide or a protein present in a sample solution. The method includes putting into contact a target compound with a capture probe and detecting signals resulting from the binding between the target compound and its corresponding capture probe and resulting from the printed detection molecule in the different discrete regions. The method obtains a detection curve of the detected signals of the detection molecule and converts the signal obtained from the target compound bound to a specific capture probe into a concentration value and quantifies the target compound by converting the concentration value into a target amount using a target concentration curve.

Abstract: The present invention relates to a method for obtaining an activation profile of a biological sample comprising disposing onto a solid support in a pre-determined spatial arrangement a subset of capture molecules able to interact with one or more activated transcription factor(s) present in the biology cell sample, contacting the biological sample upon the solid support under conditions allowing their interaction, monitoring signals resulting from their interaction, and providing a cellular activation profile from the detected signals.

Abstract: The present invention is related to a screening, detection and/or quantification method of one or more transcriptional factor(s) (1) possibly present in a biological sample, said method comprising the steps of: possibly extracting and isolating said transcriptional factor (1) from said biological sample, putting into contact the transcriptional factor (1) with a double-stranded DNA sequence (2) bound to an insoluble solid support (3), and detecting and/or quantifying said fixed transcriptional factor (1), said double-stranded DNA sequence having a specific sequence able to be fixed by the transcriptional factor (1) and being preferably located at a distance of at least about 6.8 nm from the surface of the solid support (3), and said double-stranded DNA sequence being bound to the surface of the insoluble solid support (3) at a concentration of at least 0.01 pmole/cm2 of solid support surface (3). The present invention is also related to the kit comprising means and media for performing said method.

Abstract: This invention provides a method for evaluation of the level of activation of cells through the quantification of the phosphorylated versus the non-phosphorylated multiple proteins belonging to kinase cascades which is the result of the equilibrium between the kinase/phosphatase activities in the given cells. The invention also provides a method for quantification of the level of phosphorylation of enzymes and proteins involved into the pathways of cell activation or being the products of theses activations. In particular, the invention provides a method for the simultaneous quantification level of phosphorylation of multiple proteins of the same or different pathways present in the same cell extract.

Abstract: The present invention is related to a method and kit (or device) for identifying and/or quantifying in an array mRNA nucleotide sequences related to resistance to antibiotic(s) being expressed in a (micro)organism present in a sample.

Abstract: The present invention relates to a detection and quantification method of different sets of target molecules (1, 3), being possibly present simultaneously in a biological sample by the use of a microarray present upon a solid support surface (6) and comprising different sets (A, B) of capture molecules (2, 4), present in different locations (8, 9) of the solid support surface (6).

Abstract: The present invention is related to a method for identifying and/or quantifying an organism or part of an organism in a sample by detecting a nucleotide sequence specific for said organism, among at least 4 other nucleotide sequences from other organisms or from parts of the organisms.

Abstract: The invention relates to a method and a kit for the specific identification and/or quantification of one or several among at least 7 organisms or parts thereof, in a biological sample being possibly contaminated by at least 4 other organisms, by detecting at least one nucleotide sequence specific of each of the organisms possibly present in said biological sample, wherein said nucleotide sequence is homologous with at least 4 other nucleotide sequences.

Abstract: The present invention is related to a method for the identification and/or the quantification of a target compound obtained from a sample, preferably a biological sample, comprising the steps of putting into contact the target compound with a capture molecule in order to allow a specific binding between the target compound with a capture molecule, the capture molecule being fixed upon a surface of a solid support according to an array comprising a density of at least 20 discrete regions per cm2, each of the discrete regions being fixed with one species of capture molecules, performing a reaction leading to a precipitate formed at the location of the binding, determining the possible presence of precipitate(s) in discrete region(s), and correlating the presence of the precipitate(s) at the discrete region(s) with the identification and/or a quantification of the target compound.

Abstract: The present invention is related to a method for identifying and/or quantifying an organism or part of an organism in a sample by detecting a nucleotide sequence specific of said organism, among at least 4 other nucleotide sequences from other organisms or from parts of the organism.