Abstract: The present invention refers to a culture medium and a process for producing proteases with collagenolytic and gelatinolytic activity by bacteria of the genus Clostridium. Particularly, the present invention refers to an animal product-free culture medium for C. histolyticum, characterized by comprising non-animal origin peptones, preferably vegetable peptones, yeast extract and the amino acids cysteine and arginine. The present invention also refers to a process for producing Clostridium histolyticum liquid culture supernatant comprising one or more proteases with collagenolytic and gelatinolytic activity, and pharmaceutical composition comprising as active ingredient the supernatant or the supernatant optionality purified.

Abstract: The present invention refers to a culture medium and a process for producing proteases with collagenolytic and gelatinolytic activity by bacteria of the genus Clostridium. Particularly, the present invention refers to an animal product-free culture medium for C. histolyticum, characterized by comprising non-animal origin peptones, preferably vegetable peptones, yeast extract and the amino acids cysteine and arginine. The present invention also refers to a process for producing Clostridium histolyticum liquid culture supernatant comprising one or more proteases with collagenolytic and gelatinolytic activity, and pharmaceutical composition comprising as active ingredient the supernatant or the supernatant optionality purified.

Abstract: The present invention refers to a culture medium and a process for producing Clostridium collagenolytic and gelatinolytic proteases. The present invention includes an animal product-free culture medium for C. histolyticum comprising non-animal derived peptones, preferably vegetable derived peptones, yeast extract and the amino acids cysteine and arginine. The present invention also includes a process for producing a supernatant of Clostridium histolyticum liquid culture comprising one or more collagenolytic and gelatinolytic proteases, and pharmaceutical composition comprising as active ingredient a supernatant of the Clostridium cultures described herein.

Abstract: The present invention refers to a culture medium and a process for producing proteases with collagenolytic and gelatinolytic activity by bacteria of the genus Clostridium. Particularly, the present invention refers to an animal product-free culture medium for C. histolyticum, characterized by comprising non-animal origin peptones, preferably vegetable peptones, yeast extract and the amino acids cysteine and arginine. The present invention also refers to a process for producing Clostridium histolyticum liquid culture supernatant comprising one or more proteases with collagenolytic and gelatinolytic activity, and pharmaceutical composition comprising as active ingredient the supernatant or the supernatant optionality purified.

Abstract: The present invention relates to a vector for expression of a heterologous protein by a Gram negative bacteria, wherein the vector includes a nucleic acid such as DNA encoding the following: an origin of replication region; optionally and preferably a selection marker; a promoter; an initiation region such as translation initiation region and/or a ribosome binding site, at least one restriction site for insertion of heterologous nucleic acid, e.g. DNA, encoding the heterologous protein, and a transcription terminator. The inventive vector may contain DNA encoding the heterologous protein, e.g., pro-insulin such as pro-insulin with a His tag.

Abstract: The present invention relates to a vector for expression of a heterologous protein by a Gram negative bacteria, wherein the vector includes a nucleic acid such as DNA encoding the following: an origin of replication region; optionally and preferably a selection marker; a promoter; an initiation region such as translation initiation region and/or a ribosome binding site, at least one restriction site for insertion of heterologous nucleic acid, e.g. DNA, encoding the heterologous protein, and a transcription terminator. The inventive vector may contain DNA encoding the heterologous protein, e.g., pro-insulin such as pro-insulin with a His tag.

Abstract: The present invention relates to a vector for expression of a heterologous protein by a Gram negative bacteria, wherein the vector includes a nucleic acid such as DNA encoding the following: an origin of replication region; optionally and preferably a selection marker; a promoter; an initiation region such as translation initiation region and/or a ribosome binding site, at least one restriction site for insertion of heterologous nucleic acid, e.g. DNA, encoding the heterologous protein, and a transcription terminator. The inventive vector may contain DNA encoding the heterologous protein, e.g., pro-insulin such as pro-insulin with a His tag.

Abstract: A fuel injection valve for internal combustion engines is supplied from a high-pressure fuel reservoir with high-pressure fuel that at the same time also serves to actuate the injection valve needle of the fuel injection valve. The injection valve needle is actuated via a tappet, which defines a control pressure chamber that is supplied with high-pressure fuel via a Z-throttle and can be relieved via an outlet throttle which is controlled by a magnet valve. The control pressure chamber is enclosed in an insert by the tappet guided in a cylindrical bore, and this insert receives not only the cylindrical bore but also a flat armature, a tappet of the flat armature, and a valve member of the magnet valve. The insert is braced on the housing on the injection valve by the magnet valve housing.

Abstract: The present invention relates to a vector for expression of a heterologous protein by a Gram negative bacteria, wherein the vector includes a nucleic acid such as DNA encoding the following: an origin of replication region; optionally and preferably a selection marker; a promoter; an initiation region such as translation initiation region and/or a ribosome binding site, at least one restriction site for insertion of heterologous nucleic acid, e.g. DNA, encoding the heterologous protein, and a transcription terminator. The inventive vector may contain DNA encoding the heterologous protein, e.g., pro-insulin such as pro-insulin with a His tag.

Abstract: In order to improve a device for measuring hydraulic flow quantities and leaks in a specimen, including a measurement path, embodied as an approximately vertically extending lead line to the specimen, and a capacitor sensor, which is disposed in the measurement path and can be acted upon both by at least one measuring medium and by at least one medium for generating a pressure (pressure medium) on the measuring medium in such a way that precise measurements of flow quantities and leaks are possible in a technically easily achieved way, the specimen is coupled directly to the measurement path.

Abstract: The present invention relates to a vector for expression of a heterologous protein by a Gram negative bacteria, wherein the vector includes a nucleic acid such as DNA encoding the following: an origin of replication region; optionally and preferably a selection marker; a promoter; an initiation region such as translation initiation region and/or a ribosome binding site, at least one restriction site for insertion of heterologous nucleic acid, e.g. DNA, encoding the heterologous protein, and a transcription terminator. The inventive vector may contain DNA encoding the heterologous protein, e.g., pro-insulin such as pro-insulin with a His tag.

Abstract: An apparatus for measuring hydraulic flow quantities and leaks in a specimen, including a measuring section to which the specimen is coupled and a capacitive sensor disposed in the measuring section. The measuring section can be acted upon both by at least one measurement medium and by at least one pressure medium. For generating a pressure acting on the measurement medium an inlet/outlet for the measurement medium is embodied on a side of the measuring section remote from the specimen, and an inlet/outlet for the pressure medium is disposed directly on the measuring section, on a side of the measuring section toward the specimen.

Abstract: The apparatus for measuring a hydraulic fluid flow rate and/or leak rate through an object, particularly a valve, includes an approximately vertical measurement tube, a capacitive sensor mounted on the measurement tube, an equalization vessel extending vertically next to the measurement tube, and a source of hydraulic fluid connected to the equalization vessel and the measurement tube below the capacitive sensor and a source of a pneumatic medium connected to both the equalization vessel and the measurement tube above the capacitive sensor. Hydraulic and pneumatic valves are also provided so that hydraulic fluid can be accommodated in a lower part of the equalization vessel and pneumatic medium in an upper part. A connector tube with a connector valve is connected to the equalization vessel at about the level of the capacitive sensor so that the level of hydraulic fluid can be set at the capacitive sensor in the measurement tube at the beginning of the measurement.

Abstract: The invention relates to a dry, surfactant-free single-component alkali metal silicate cement composition containing as hardening agent condensed aluminum phosphate and at least one compound from the group comprising the ortho- and pyrophosphates of manganese, zinc, cobalt, iron(III) and chromium(III) and in addition containing the conventional components rounded quartz sand, clay, kieselguhr, mineral oil and solid powdered sodium water glass or potassium water glass.

Abstract: A dry one-component alkali metal silicate cement composition contains the following components:(a) condensed aluminum phosphate or condensed aluminum/iron phosphate as hardener in amounts between 2-10% by weight,(b) rounded quartz sand having a grain size between 0.01 and 0.5 mm in amounts of 30-70% by weight,(c) clay constituents in amounts of 10-40% by weight,(d) kieselguhr in amounts of 1-5% by weight,(e) mineral oil in amounts of 0.05-1% by weight and(f) solid pulverulent sodium silicate, potassium silicate or mixtures thereof in amounts of 10-25% by weight.The composition is free of surfactant. When combined with water the cement is slow to set into an acid-resistant mortar.