Patents by Inventor Josef Ruschoff

Josef Ruschoff has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of primer-extension preamplification PCR
    Publication number: 20020160404
    Abstract: The invention provides a method for the amplification of nucleic acid fragments from a sample that comprises two or three thermocyclic amplification reactions, whereby completely randomized primers are used in the first amplification reaction and specific primers are used in the second amplification reaction, characterized in that, to amplify the DNA, a mixture of at least two DNA polymerases is used, at least one of which possesses proofreading activity. Using this method, DNA from single cells or cell clones with a low cell count can be used in mutation analysis.
    Type: Application
    Filed: February 28, 2002
    Publication date: October 31, 2002
    Applicant: Roche Molecular Systems, Inc.
    Inventors: Wolfgang Dietmaier, Josef Ruschoff
  • Method of primer-extension preamplification PCR
    Patent number: 6365375
    Abstract: The invention provides a method for the amplification of nucleic acid fragments from a sample that comprises two or three thermocyclic amplification reactions, whereby completely randomized primers are used in the first amplification reaction and specific primers are used in the second amplification reaction, characterized in that, to amplify the DNA, a mixture of at least two DNA polymerases is used, at least one of which possesses proofreading activity. Using this method, DNA from single cells or cell clones with a low cell count can be used in mutation analysis.
    Type: Grant
    Filed: March 16, 1999
    Date of Patent: April 2, 2002
    Inventors: Wolfgang Dietmaier, Josef Ruschoff
  • Method for the detection of microsatellite instability for tumor diagnostics
    Patent number: 6150100
    Abstract: The invention concerns a method for the determination of the genomic instability at 5 selected microsatellite loci. The analysis of these selected loci is suitable for making prognostic tumour diagnoses, for analysing hereditary tumor predisposition as well as for early tumor detection. This method is of particular importance for the diagnosis of tumors of the gastrointestinal tract such as colorectal tumors.
    Type: Grant
    Filed: March 25, 1998
    Date of Patent: November 21, 2000
    Assignee: Roche Diagnostics GmbH
    Inventors: Josef Ruschoff, Wolfgang Dietmaier, Richard Fishel