Abstract: The present invention provides a non-naturally occurring composition comprising a CRISPR nuclease comprising a sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 3 or a nucleic acid molecule comprising a sequence encoding the CRISPR nuclease.

Abstract: A flexure acts as an interface for mounting a precision sensing device to a support having a different coefficient of thermal expansion. The flexure, which has substantially the same coefficient of expansion as the device, engages the support along an annular contacting surface to restrain the flexure from relative slipping movement under thermal stress. The sensing device is carried on a base of the flexure and therefore remains aligned during thermal cycling. In a preferred embodiment, the contacting surface provides tight surface-to-surface contact with a complementary surface of the support over an operating temperature range.