Abstract: Electrophysiology mapping systems and methods that are used to determine longitudinal and transverse conduction velocities within myocardial tissue. The myocardial tissue can be contacted with at least three non-collinear electrodes. A selected electrical pacing protocol can include at least one pacing wave that is delivered to the at least three non-collinear electrodes. During each pacing wave, at least one pair of adjacent electrodes can generate an electrical activation pattern, and at least one additional electrode can detect the activation pattern. For each pacing wave, a processor determines a conduction velocity vector associated with the detection of the electrical activation pattern at a corresponding electrode. The processor can determine a singular value decomposition of the conduction velocity vectors determined during the electrical pacing protocol and use the singular value decomposition to determine the longitudinal and transverse conduction velocities within the myocardial tissue.