Abstract: Glycerol is a byproduct of biodiesel and bioethanol production and its conversion to value-added chemicals is a promising avenue for realization of the biorefinery concept Conversion of glycerol to pyruvate through glycerate yields pyruvate, is a common intermediate of many high-value natural products. The present invention aims at improving the specific activity of a naturally occurring enzyme toward conversion of glycerate to pyruvate (TvDHT). The present invention features compositions of isolated dehydratase enzyme (DHT) polypeptide composition, in particular the DHT polypeptide composition comprising a single point mutation that increases the specific activity of the enzyme as compared to wild type.

Abstract: The invention is directed to methods of enzymatic production of betalains, including betalamic acid, in cell-free medium.

Abstract: A mobile device holder that includes a body having a plurality of cavities. The mobile device holder includes a first assembly, a second assembly, and a third assembly. The first assembly is coupled to the body. The first assembly is configurable to be coupled to different portions of the body. The first assembly includes a first arm configured to be coupled to the body, a first slider coupled to the first arm, where the first slider is configured to move along the first arm, a first cap coupled to the first arm, and a first screw coupled to the first cap, where the first screw is configured to move the first slider along the first arm and towards the body.

Abstract: The invention is directed to methods involved in the production of flavonoids, anthocyanins and other organic compounds. The invention provides cells engineered for the production of flavonoids, anthocyanins and other organic compounds, where the engineered cells include one or more genetic modifications that increase flavonoid production by increasing metabolic flux to flavonoid precursors and/or reducing carbon losses resulting from the production of byproducts.

Abstract: The invention is directed to methods involved in the production of flavonoids, anthocyanins and other organic compounds. The invention provides cells engineered for the production of flavonoids, anthocyanins and other organic compounds, where the engineered cells include one or more genetic modifications that increase flavonoid production by increasing metabolic flux to flavonoid precursors and/or reducing carbon losses resulting from the production of byproducts.

Abstract: The invention is directed to methods involved in the production of flavonoids, anthocyanins and other organic compounds. The invention provides cells engineered for the production of flavonoids, anthocyanins and other organic compounds, where the engineered cells include one or more genetic modifications that increase flavonoid production by increasing metabolic flux to flavonoid precursors and/or reducing carbon losses resulting from the production of byproducts.

Abstract: The invention is directed to methods involved in the production of flavonoids, anthocyanins and other organic compounds. The invention provides cells engineered for the production of flavonoids, anthocyanins and other organic compounds, where the engineered cells include one or more genetic modifications that increase flavonoid production by increasing metabolic flux to flavonoid precursors and/or reducing carbon losses resulting from the production of byproducts.

Abstract: The invention is directed to methods involved in the production of flavonoids, anthocyanins and other organic compounds. The invention provides cells engineered for the production of flavonoids, anthocyanins and other organic compounds, where the engineered cells include one or more genetic modifications that increase flavonoid production by increasing metabolic flux to flavonoid precursors and/or reducing carbon losses resulting from the production of byproducts.

Abstract: Glycerol is a byproduct of biodiesel and bioethanol production and its conversion to value-added chemicals is a promising avenue for realization of the biorefinery concept Conversion of glycerol to pyruvate through glycerate yields pyruvate, is a common intermediate of many high-value natural products. The present invention aims at improving the specific activity of a naturally occurring enzyme toward conversion of glycerate to pyruvate (TvDHT). The present invention features compositions of isolated dehydratase enzyme (DHT) polypeptide composition, in particular the DHT polypeptide composition comprising a single point mutation that increases the specific activity of the enzyme as compared to wild type.

Abstract: Geranyl pyrophosphate (GPP) is a key intermediate molecule in the bioproduction of thousands of natural products. Currently, natural products are either cultivated from plants, synthesized via complex chemical synthesis strategies, or through cell-based factories also known as biofoundries. However, in order to replicate the process in a cell free environment, numerous enzymes and cofactors must be utilized making this approach costly and unviable. In order to make this process viable, a new approach was needed that uses fewer enzymes and cofactors. As described herein, the present invention demonstrates that it is possible to create GPP from glycerol through a short and concise biosynthetic pathway outside of the cell.

Abstract: The present invention features a versatile continuous manufacturing platform for cell-free chemical production.

Abstract: Methods and apparatus are disclosed for near field radio-frequency (RF) testing of devices, particularly user equipment (UEs) capable of millimeter-wave (mmWave) transmissions. An exemplary test apparatus is described that uses a transducer to facilitate near field over-the-air testing of UEs in the mmWave transmission band. The transducer may be an orthomode transducer and may include a dual-polarity port positioned in the reactive near field of an antenna of a device under test (DUT). For UE signal transmission tests, the orthomode transducer splits test signals received from the antenna of the DUT via the dual-polarity port into a pair of single-polarity RF signals. The single-polarity RF signals are separately fed through a pair of waveguide-to-coaxial adaptors into separate coaxial cables, which feed coaxial transmission versions of the single-polarity RF signals to test equipment for analysis. UE signal reception tests are also described that utilize the same or different orthomode transducer.

Abstract: There are provided, inter alia, methods for reacting an enzyme and its substrate, methods for purifying a protein and an enzyme reactor and its use thereof.

Abstract: A mobile device holder that includes a body having a plurality of cavities. The mobile device holder includes a first assembly, a second assembly, and a third assembly. The first assembly is coupled to the body. The first assembly is configurable to be coupled to different portions of the body. The first assembly includes a first arm configured to be coupled to the body, a first slider coupled to the first arm, where the first slider is configured to move along the first arm, a first cap coupled to the first arm, and a first screw coupled to the first cap, where the first screw is configured to move the first slider along the first arm and towards the body.

Abstract: There are provided, inter alia, methods for reacting an enzyme and its substrate, methods for purifying a protein and an enzyme reactor and its use thereof.

Abstract: A remote speaker microphone having a directional microphone, a speaker, and a housing. The housing has a first portion sized to contain the speaker and a second portion sized to contain the directional microphone. The second portion defines a front port positioned adjacent the front surface of the directional microphone and a rear port positioned adjacent the rear surface of the directional microphone. The housing is configured to minimize the distance that sound waves travel within the housing before impacting on the sound receiving surfaces of the microphone.

Abstract: A remote speaker microphone having a directional microphone, a speaker, and a housing. The housing has a first portion sized to contain the speaker and a second portion sized to contain the directional microphone. The second portion defines a front port positioned adjacent the front surface of the directional microphone and a rear port positioned adjacent the rear surface of the directional microphone. The housing is configured to minimize the distance that sound waves travel within the housing before impacting on the sound receiving surfaces of the microphone.