Patents by Inventor Joshua K Young
Joshua K Young has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230407320Abstract: Methods and compositions are provided for the transient expression and activity of site-specific DNA modifying agent, morphogenic factors or developmental genes, either alone or in combination for eukaryotic cells. The morphogenic factor and DNA modifying agent may be provided to the same cell or to a different cell than that which was originally transformed. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. Exogenously provided DNA modifying agents and/or morphogenic factors need not be segregated away in future generations due to transient activities in the desired cell.Type: ApplicationFiled: October 27, 2021Publication date: December 21, 2023Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: MAREN L. ARLING, WILLIAM JAMES GORDON-KAMM, JOSHUA K. YOUNG
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Publication number: 20230392135Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using novel engineered Cas endonucleases. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins.Type: ApplicationFiled: October 13, 2021Publication date: December 7, 2023Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: CLARA ALARCON, STEPHEN LAWRENCE GASIOR, ZHENGLIN HOU, ELIZABETH SOMMERS VAN GINKEL, JOSHUA K. YOUNG
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Patent number: 11827894Abstract: The present disclosure involves the production of Waxy maize. Compositions and methods are provided for knocking out expression of the Waxy (Wx1) gene in maize by making double strand breaks at one or more target sites in an endogenous WX1 encoding sequence. Some methods employ one or more guide polynucleotides and a Cas endonuclease, wherein Cas endonuclease is guided by the one or more guide polynucleotides to recognize and introduce double strand breaks at specific target sites in and around the Wx1 gene. Also provided are compositions and methods for the production of Waxy maize plant cells, plant explants, seeds and grain.Type: GrantFiled: September 2, 2021Date of Patent: November 28, 2023Inventors: Andrew Mark Cigan, Mark Jacob Gadlage, Huirong Gao, Robert B Meeley, Joshua K Young
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Patent number: 11807878Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using a novel Cas endonuclease. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins.Type: GrantFiled: January 21, 2021Date of Patent: November 7, 2023Inventors: Zhenglin Hou, Tautvydas Karvelis, Virginijus Siksnys, Joshua K Young
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Publication number: 20230323374Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.Type: ApplicationFiled: November 15, 2022Publication date: October 12, 2023Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INCInventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, L. ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
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Publication number: 20230279413Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.Type: ApplicationFiled: November 16, 2022Publication date: September 7, 2023Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INCInventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, L. ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
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Patent number: 11732251Abstract: Methods and compositions are provided for the use of anti-CRISPR (ACR) proteins in plants, including modulation of Cas endonuclease activity, improvement of frequency of homologous recombination, control of Cas endonuclease activity during various cell cycles, spatial and/or temporal regulation of Cas endonuclease activity in plants, usage in gene activation or repression, as well as reduction of off-target polynucleotide cleavage.Type: GrantFiled: April 24, 2018Date of Patent: August 22, 2023Assignees: DUPONT NUTRITION BIOSCIENCES APS, UNIVERSITE LAVAL (CANADA)Inventors: Christophe Fremaux, Philippe Horvath, Alexander Hynes, Marie-Laurence Lemay, Sylvain Moineau, Dennis A. Romero, Geneviève Rousseau, Joshua K Young
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Publication number: 20230235345Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.Type: ApplicationFiled: November 17, 2022Publication date: July 27, 2023Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INCInventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, L. ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
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Publication number: 20230220407Abstract: The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.Type: ApplicationFiled: January 25, 2023Publication date: July 13, 2023Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANYInventors: CLARA MARIA ALARCON, MATTHEW C. HARMON, M. ALEJANDRA PASCUAL, JAMES C. REGISTER, III, CHRISTOPHER J. SCELONGE, JOSHUA K. YOUNG, CATHY XIAOYAN ZHONG
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Publication number: 20230183724Abstract: Methods and compositions are provided for massively parallel base editing in the genome of an organism, such as a plant. Multiple loci are selected based on one or more specific criteria, such as evolutionary conservation, recombination frequency, nonsense mutations, and/or missense mutations. Predictive models for deleterious alleles and biologically-informed phenotype prediction may be developed and refined based on the editing results, and improved plant lines for commercial or breeding purposes may be obtained.Type: ApplicationFiled: February 3, 2021Publication date: June 15, 2023Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: NICHOLAS DOANE CHILCOAT, JOSEPH EVANS, YI JIA, ELI RODGERS-MELNICK, JOSHUA K YOUNG
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Publication number: 20230119655Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using a novel Cas endonuclease. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins.Type: ApplicationFiled: December 7, 2022Publication date: April 20, 2023Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ZHENGLIN HOU, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K YOUNG
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Publication number: 20230084762Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using a novel Cas endonuclease. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins.Type: ApplicationFiled: February 11, 2021Publication date: March 16, 2023Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: GIEDRIUS GASIUNAS, ZHENGLIN HOU, TOMAS URBAITIS, JOSHUA K YOUNG
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Publication number: 20220356485Abstract: Compositions and methods are provided for rapid characterization of Cas endonuclease systems and the elements comprising such systems, including, but not limiting to, rapid characterization of PAM sequences, guide RNA elements and Cas endonucleases. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri MIc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.Type: ApplicationFiled: May 19, 2022Publication date: November 10, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, GIEDRIUS GASIUNAS, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K. YOUNG
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Publication number: 20220251587Abstract: Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.Type: ApplicationFiled: April 11, 2022Publication date: August 11, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: STEPHEN LAWRENCE GASIOR, WILLIAM JAMES GORDON-KAMM, KEITH S LOWE, DAVID J PETERSON, SERGEI SVITASHEV, JOSHUA K YOUNG
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Patent number: 11371050Abstract: Compositions and methods are provided for rapid characterization of Cas endonuclease systems and the elements comprising such systems, including, but not limiting to, rapid characterization of PAM sequences, guide RNA elements and Cas endonucleases. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri Mlc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.Type: GrantFiled: May 12, 2016Date of Patent: June 28, 2022Inventors: Andrew Mark Cigan, Giedrius Gasiunas, Tautvydas Karvelis, Virginijus Siksnys, Joshua K Young
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Publication number: 20220177900Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell. Breeding methods and methods for selecting plants utilizing a two component RNA polynucleotide and Cas endonuclease system are also disclosed.Type: ApplicationFiled: February 22, 2022Publication date: June 9, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, PHILLIP A PATTEN, JOSHUA K YOUNG
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Publication number: 20220162628Abstract: The present disclosure involves the production of Waxy maize. Compositions and methods are provided for knocking out expression of the Waxy (Wx1) gene in maize by making double strand breaks at one or more target sites in an endogenous WX1 encoding sequence. Some methods employ one or more guide polynucleotides and a Cas endonuclease, wherein Cas endonuclease is guided by the one or more guide polynucleotides to recognize and introduce double strand breaks at specific target sites in and around the Wx1 gene. Also provided are compositions and methods for the production of Waxy maize plant cells, plant explants, seeds and grain.Type: ApplicationFiled: September 2, 2021Publication date: May 26, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, MARK JACOB GADLAGE, HUIRONG GAO, ROBERT B MEELEY, JOSHUA K YOUNG
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Patent number: 11332752Abstract: Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.Type: GrantFiled: March 11, 2019Date of Patent: May 17, 2022Assignee: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Stephen Lawrence Gasior, William James Gordon-Kamm, Keith S Lowe, David J Peterson, Sergei Svitashev, Joshua K Young
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Publication number: 20220073890Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using a novel Cas endonuclease. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins.Type: ApplicationFiled: December 13, 2019Publication date: March 10, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ZHENGLIN HOU, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K YOUNG
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Publication number: 20220010293Abstract: Compositions and methods are provided for novel Cas9 orthologs, including, but not limiting to, novel guide polynucleotide/Cas9 endonucleases complexes, single or dual guide RNAs, guide RNA elements, and Cas9 endonucleases. The present disclosure also describes methods for creating a double strand break in a target polynucleotide, methods for genome modification of a target sequence under various in vivo and in vitro conditions, in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell. Also provided are nucleic acid constructs and cells having a modified target site or altered polynucleotide of interest produced by the methods described herein.Type: ApplicationFiled: February 22, 2019Publication date: January 13, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ZHENGLIN HOU, JOSHUA K YOUNG, GIEDRIUS GASIUNAS, VIRGINIJUS SIKSNYS