Patents by Inventor Joshua L. Cherry

Joshua L. Cherry has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Use of primers containing non-replicatable residues for improved cycle-sequencing of nucleic acids
    Publication number: 20030049657
    Abstract: The present invention provides primers for use in cycle sequencing which are not subject to exponential amplification of undesired artifacts. Such primers cannot be replicated by the nucleic acid polymerases used in these reactions and, therefore, do not produce artifacts. Methods of linear amplification of a nucleic acid template using such primers are also provided.
    Type: Application
    Filed: May 15, 2002
    Publication date: March 13, 2003
    Inventor: Joshua L. Cherry
  • AUTOMATED HYBRIDIZATION/IMAGING DEVICE FOR FLUORESCENT MULTIPLEX DNA SEQUENCING
    Publication number: 20020012910
    Abstract: A method is disclosed for automated multiplex sequencing of DNA with an integrated automated imaging hybridization chamber system. This system comprises an hybridization chamber device for mounting a membrane containing size-fractionated multiplex sequencing reaction products, apparatus for fluid delivery to the chamber device, imaging apparatus for light delivery to the membrane and image recording of fluorescence emanating from the membrane while in the chamber device, and programmable controller apparatus for controlling operation of the system. The multiplex reaction products are hybridized with a probe, then an enzyme (such as alkaline phosphatase) is bound to a binding moiety on the probe, and a fluorogenic substrate (such as a benzothiazole derivative) is introduced into the chamber device by the fluid delivery apparatus.
    Type: Application
    Filed: November 28, 1995
    Publication date: January 31, 2002
    Inventors: ROBERT B. WEISS, ALVIN W. KIMBALL, RAYMOND F. GESTELAND, F. MARK FERGUSON, DIANE M. DUNN, LEONARD J. DI SERA, JOSHUA L. CHERRY
  • Automated hybridization/imaging device for fluorescent multiplex DNA sequencing
    Patent number: 5470710
    Abstract: A method is disclosed for automated multiplex sequencing of DNA with an integrated automated imaging hybridization chamber system. This system comprises an hybridization chamber device for mounting a membrane containing size-fractionated multiplex sequencing reaction products, apparatus for fluid delivery to the chamber device, imaging apparatus for light delivery to the membrane and image recording of fluorescence emanating from the membrane while in the chamber device, and programmable controller apparatus for controlling operation of the system. The multiplex reaction products are hybridized with a probe, then an enzyme (such as alkaline phosphatase) is bound to a binding moiety on the probe, and a fluorogenic substrate (such as a benzothiazole derivative) is introduced into the chamber device by the fluid delivery apparatus.
    Type: Grant
    Filed: October 22, 1993
    Date of Patent: November 28, 1995
    Assignee: University of Utah
    Inventors: Robert B. Weiss, Alvin W. Kimball, Raymond F. Gesteland, F. Mark Ferguson, Diane M. Dunn, Leonard J. Di Sera, Joshua L. Cherry