Abstract: Provided is a bioreactor apparatus including: a liquid storage chamber for storing a culture medium; a pump for providing pressure to drive the flow of the culture medium; a plurality of culture chambers providing an accommodating space to accommodate the culture medium and a cell to be cultured; and a pipeline connecting the liquid storage chamber, the pump, and the culture chamber to form a closed loop. Also provided is a bioreactor system including the bioreactor apparatus of the present disclosure, the culture medium, and a cell. Further provided is a method for culturing a cell or an organoid by the bioreactor apparatus of the present disclosure. The bioreactor apparatus, bioreactor system, and method of the present disclosure can form a biomimetic circulatory system, which is beneficial for simulating and evaluating the complex microenvironment and physiological mechanism in the living body.

Abstract: A hydrogel composition, a hydrogel biomedical material, a method for facilitating regeneration of a bone and a manufacturing method of a hydrogel composition are provided. The hydrogel composition includes a first deionized water, a gel powder, a transglutaminase mixture and a hyaluronic acid powder. The gel powder includes gelatin and alginic acid. The first deionized water, the gel powder, the transglutaminase mixture and the hyaluronic acid powder are evenly mixed. Based on the hydrogel composition being 100 wt %, the first deionized water is 95 wt % to 98.46 wt %, the gel powder is 1 wt % to 3 wt %, the transglutaminase mixture is 0.04 wt % to 0.15 wt %, and the hyaluronic acid powder is 0.5 wt % to 1.5 wt %.

Abstract: An osteo-implant is provided, having a fixing segment and an implant end. The fixing segment is provided with a thread and at least one bone healing chamber. The bone healing chamber is located at a thread root of the thread. The bone healing chamber is a chamber inwardly depressed from a surface of the fixing segment. The fixing segment has a central axis. In the same section of the fixing segment, an area of a section, which is parallel to and passes through the central axis, of the at least one bone healing chamber closer to the implant end is smaller.

Abstract: The present invention relates to a three-dimensional (3D) tissue-like implant for transplanting to a subject in need comprising a cell cluster comprising mesenchymal stem cells (MSCs) and specific cells differentiated therefrom. The present invention also relate to a method of preparing a 3D-tissue-like implant from MSCs, particularly by seeding MSCs in alginate scaffolds and culturing the alginate scaffolds with MSCs in a 3-D perfusion condition. Further, the present invention provides a method for treating a defect in a recipient patient in need by administering a 3D tissue-like implant as described herein to the patient at a defective site e.g. a bone defective site.

Abstract: A bionic fixing apparatus is provided. The bionic fixing apparatus includes a body having a through hole and at least one slit. The through hole penetrates the body from the top surface to the bottom surface to form a top opening and a bottom opening. An inner diameter of the top opening is larger than an inner diameter of the bottom opening. The slit is connected to the bottom opening and extends upwardly from the bottom surface of the body, such that the body has a flexible bottom portion.

Abstract: A bionic apparatus is provided. The bionic apparatus includes a flexible portion having a plurality of pores, a rigid portion connected with the flexible portion, and a supporting element disposed in the flexible portion. The pore size of each pore is between 50 ?m to 500 ?m. The flexible portion, the rigid portion and the supporting element are one-piece formed by a additive manufacturing process.

Abstract: An expandable orthopedic implant includes a casing pipe, a shaft, a first expandable element and a first link lever. The casing pipe has a first opening, a second opening and an axis passing through the first and second opening. The casing pipe sheathes one end of the shaft, and the other end of the shaft has a top portion disposed out of the first opening. The first expandable element has a first terminal pivoted on the casing pipe and a second terminal. The first link lever has a proximal end pivoted to the top portion and a distal end pivoted to the second terminal. When the top portion is driven moving away from the first opening along an extending direction of the axis, the first link-lever can be pushed by the shaft to enact the second terminal moving away from the axis along a direction perpendicular to the axis.

Abstract: A nerve impulse signal stimulation device and a method for fabricating the same are provided. The nerve impulse signal stimulation device includes: a substrate having a first surface and a second surface opposite to the first surface; a first metal layer formed on the first surface of the substrate; a second metal layer formed on the first metal layer; a plurality of openings exposing a portion of the first surface of the substrate, a portion of the first metal layer and a portion of the second metal layer; and a ferromagnetic material attached to the second surface of the substrate. The openings cause the nerve impulse signal stimulation device to obtain a parallel circuit structure, thereby increasing the current load and the magnetic field intensity, reducing the size of the device, and ensuring the safety of operations.

Abstract: The present invention is related to a kit comprising: (a) a mesenchymal stem cell; (b) a gelatin-hyaluronan-chondroitin tri-copolymer scaffold; (c) a kartogenin; and (d) a bioreactor. The present invention is also related to a method for promoting differentiation of a mesenchymal stem cell into cartilage tissue, comprising: (a) culturing the mesenchymal stem cell on a gelatin- hyaluronan-chondroitin tri-copolymer scaffold in the presence of a kartogenin; and (b) culturing the mesenchymal stem cell and the gelatin-hyaluronan-chondroitin tri-copolymer scaffold in a bioreactor.

Abstract: An osteo-implant is provided, having a fixing segment and an implant end. The fixing segment is provided with a thread and at least one bone healing chamber. The bone healing chamber is located at a thread root of the thread. The bone healing chamber is a chamber inwardly depressed from a surface of the fixing segment. The fixing segment has a central axis. In the same section of the fixing segment, an area of a section, which is parallel to and passes through the central axis, of the at least one bone healing chamber closer to the implant end is smaller.

Abstract: A bionic fixing apparatus is provided. The bionic fixing apparatus includes a flexible portion having at least one trench. The trench is disposed on the surface of the flexible portion and has a first end and a second end. An interval is disposed between the first end and the second end. The trench is disposed for spreading the stress applied on the bionic fixing apparatus and preventing stress concentration and stress shielding.

Abstract: A nerve impulse signal stimulation device and a method for fabricating the same are provided. The nerve impulse signal stimulation device includes: a substrate having a first surface and a second surface opposite to the first surface; a first metal layer formed on the first surface of the substrate; a second metal layer formed on the first metal layer; a plurality of openings exposing a portion of the first surface of the substrate, a portion of the first metal layer and a portion of the second metal layer; and a ferromagnetic material attached to the second surface of the substrate. The openings cause the nerve impulse signal stimulation device to obtain a parallel circuit structure, thereby increasing the current load and the magnetic field intensity, reducing the size of the device, and ensuring the safety of operations.

Abstract: The present invention is related to a kit comprising: (a) a mesenchymal stem cell; (b) a gelatin-hyaluronan-chondroitin tri-copolymer scaffold; (c) a kartogenin; and (d) a bioreactor. The present invention is also related to a method for promoting differentiation of a mesenchymal stem cell into cartilage tissue, comprising: (a) culturing the mesenchymal stem cell on a gelatin- hyaluronan-chondroitin tri-copolymer scaffold in the presence of a kartogenin; and (b) culturing the mesenchymal stem cell and the gelatin-hyaluronan-chondroitin tri-copolymer scaffold in a bioreactor.

Abstract: The present invention provides a method for fabricating a cell-laden hydrogel construct, comprising the steps of: (a) making a mixture of a physically gelable protein, a first crosslinking enzyme and a cell; (b) extruding the mixture into a second crosslinking enzyme before the mixture is gelled and forming a microgel by physical gelling; and (c) reacting the microgel with the second crosslinking enzyme to fabricate the cell-laden hydrogel construct.

Abstract: A bionic apparatus is provided. The bionic apparatus includes a flexible portion having a plurality of pores, a rigid portion connected with the flexible portion, and a supporting element disposed in the flexible portion. The pore size of each pore is between 50 ?m to 500 ?m. The flexible portion, the rigid portion and the supporting element are one-piece formed by a additive manufacturing process.

Abstract: A bionic fixing apparatus is provided. The bionic fixing apparatus includes a body having a through hole and at least one slit. The through hole penetrates the body from the top surface to the bottom surface to form a top opening and a bottom opening. An inner diameter of the top opening is larger than an inner diameter of the bottom opening. The slit is connected to the bottom opening and extends upwardly from the bottom surface of the body, such that the body has a flexible bottom portion.

Abstract: A bionic fixing apparatus is provided. The bionic fixing apparatus includes a flexible portion having at least one trench. The trench is disposed on the surface of the flexible portion and has a first end and a second end. An interval is disposed between the first end and the second end. The trench is disposed for spreading the stress applied on the bionic fixing apparatus and preventing stress concentration and stress shielding.

Abstract: Thermal responsive compositions for treating bone diseases are provided. The thermal responsive composition for treating bone diseases includes a bone growth factor and a biodegradable copolymer. The biodegradable copolymer has a structure of Formula (I) or Formula (II): A-B-BOX-B-A??Formula (I) B-A-B-(BOX-B-A-B)n-BOX-B-A-B??Formula (II) wherein, A includes a hydrophilic polyethylene glycol polymer, B includes a hydrophobic polyester polymer, BOX is a bifunctional group monomer of 2, 2?-Bis(2-oxazoline) and used for coupling the blocks A-B or B-A-B, and n is an integer and the same or more than 0.

Abstract: Thermal responsive compositions for treating bone diseases are provided. The thermal responsive composition for treating bone diseases includes a bone growth factor and a biodegradable copolymer. The biodegradable copolymer has a structure of Formula (I) or Formula (II): A-B-BOX-B-A??(Formula I) and B-A-B-(BOX-B-A-B)n-BOX-B-A-B??Formula (II), wherein, A includes a hydrophilic polyethylene glycol polymer, B includes a hydrophobic polyester polymer, BOX is a bifunctional group monomer of 2,2?-Bis(2-oxazoline) and used for coupling the blocks A-B or B-A-B, and n is an integer and the same or more than 0.

Abstract: A spinal fusion device abutting and fixed between adjacent vertebrae. The spinal fusion device includes a support frame. The support frame has a plurality of extending bodies, each radially extending from a center of the support frame. A predetermined gap exists between every two adjacent extending bodies, receiving a bone graft.