Patents by Inventor Juichi Saitoh
Juichi Saitoh has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20060228698Abstract: An oligonucleotide useful for detection of an RNA derived from HIV-1 consisting of at least 10 consecutive bases in any of SEQ ID NOS:1, 2, 4 to 10 and 13 to 17, which can bind to a specific site of the RNA.Type: ApplicationFiled: January 27, 2006Publication date: October 12, 2006Applicant: Tosoh CorporationInventors: Tetsuya Ishizuka, Takahiko Ishiguro, Juichi Saitoh
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Patent number: 7112407Abstract: A simple and accurate method for assay of a single-stranded RNA containing a specific nucleic acids sequence in a sample at almost constant temperature by using at least the following reagents (A) to (I), which comprises a step of adding the reagents (A) to (I) one by one (in any order), in combinations of at least two or all at once and a step of measuring a fluorescent signal in the presence of the reagent (I) at least once after addition of at least the reagents (A) to (H); (A) a first single-stranded oligonucleic acid complementary to a sequence neighboring the 5? end of the specific nucleic acids sequence in the single-stranded RNA, (B) a second single-stranded oligo DNA complementary to a 3?-end sequence within the specific nucleic acids sequence, (C) an RNA-dependent DNA polymerase, (D) deoxyribonucleoside triphosphates, (E) a third single-stranded oligo DNA having (1) a promoter sequence for a DNA-dependent RNA polymerase, (2) an enhancer sequence for the promoter and (3) a 5?-end sequence within theType: GrantFiled: October 20, 2003Date of Patent: September 26, 2006Assignee: Tosoh CorporationInventors: Takahiko Ishiguro, Juichi Saitoh, Tetsuya Ishizuka
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Patent number: 7049067Abstract: An oligonucleotide useful for detection of an RNA derived from HIV-1 consisting of at least 10 consecutive bases in any of SEQ ID NOS:1, 2, 4 to 10 and 13 to 17, which can bind to a specific site of the RNA.Type: GrantFiled: October 30, 2001Date of Patent: May 23, 2006Assignee: Tosoh CorporationInventors: Tetsuya Ishizuka, Takahiko Ishiguro, Juichi Saitoh
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Publication number: 20040115718Abstract: A simple and accurate method for assay of a single-stranded RNA containing a specific nucleic acids sequence in a sample at almost constant temperature by using at least the following reagents (A) to (I), which comprises a step of adding the reagents (A) to (I) one by one (in any order), in combinations of at least two or all at once andType: ApplicationFiled: October 20, 2003Publication date: June 17, 2004Applicant: TOSOH CORPORATIONInventors: Takahiko Ishiguro, Juichi Saitoh, Tetsuya Ishizuka
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Publication number: 20040048246Abstract: An oligonucleotide useful for detection of an RNA derived from HIV-1 consisting of at least 10 consecutive bases in any of SEQ ID NOS:1, 2, 4 to 10 and 13 to 17, which can bind to a specific site of the RNA.Type: ApplicationFiled: October 30, 2001Publication date: March 11, 2004Inventors: Tetsuya Ishizuka, Takahiko Ishiguro, Juichi Saitoh
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Publication number: 20040014119Abstract: A method of detecting at least two nucleic acids in a sample, which comprises allowing the respective nucleic acids to form duplexes having different Tm values with nucleic acid probes complementary to the respective nucleic acids which are labeled with the same fluorescent substance and detecting the respective nucleic acids in one vessel simultaneously on the basis of the different Tm values of the duplexes.Type: ApplicationFiled: June 20, 2003Publication date: January 22, 2004Applicant: TOSOH CORPORATIONInventors: Shun-Ichi Itoh, Toshinori Hayashi, Juichi Saitoh, Takahiko Ishiguro
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Publication number: 20040005616Abstract: A method of isolating nucleic acid in a sample, which comprises preparing an aqueous solution comprising the sample, a first water-soluble polymer, a second water-soluble polymer capable of forming two aqueous phases with the first water-soluble polymer, and a surfactant, and keeping the aqueous solution still to let the solution separate into two aqueous phases, and separating the nucleic acid and the other components in different phases.Type: ApplicationFiled: May 23, 2003Publication date: January 8, 2004Inventors: Juichi Saitoh, Yoshifumi Kurihara, Takahiko Ishiguro
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Patent number: 6664113Abstract: A method for detecting fluorescence from a liquid sample housed in a transparent or translucent sample container includes providing a sample container in a sample holder, which is opaque except for a sample container introduction opening and incoming and outgoing openings for excitation light; layering a liquid sample and a shielding liquid unmixable therewith to prevent external light from entering through the sample container introduction opening; introducing excitation light from such a direction that the excitation light can irradiate the liquid sample before irradiating the shielding liquid; and detecting fluorescence, which emits in a direction of avoiding absorption by the shielding liquid.Type: GrantFiled: April 22, 2002Date of Patent: December 16, 2003Assignee: Tosoh CorporationInventors: Yoshifumi Kurihara, Toshinori Hayashi, Juichi Saitoh, Takahiko Ishiguro
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Publication number: 20030176687Abstract: An oligonucleotide for detection or amplification of a gene selected from the group consisting of Vibrio parahaemolyticus thermostable direct hemolysin-related hemolysin genes (trh1 and trh2) and Vibrio parahaemolyticus thermostable direct hemolysin gene (tdh2) or RNA derived therefrom is provided. Further, method for detecting trh1, trh2 or tdh2 using said oligonucleotide is provided.Type: ApplicationFiled: April 7, 2003Publication date: September 18, 2003Inventors: Tetsuya Ishizuka, Takahiko Ishiguro, Juichi Saitoh
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Patent number: 6586178Abstract: A nucleic acid probe which is a single-stranded nucleic acid complementary to a specific nucleic acid sequence and is labeled so as to give off a measurable fluorescent signal on hybridization with a nucleic acid containing the specific nucleic acid sequence, wherein the 3′ end of the probe is modified as represented by the following formula (1): wherein R is —COOH, —CONH2, —(CH2)nOH, —CH(OH)—CH2OH, or —CH[(CH2)n—NHR1]—CH2OH, R1 is H, a dye, or an amino protecting group such as Fmoc, and n is an integer of at least 1.Type: GrantFiled: March 6, 2000Date of Patent: July 1, 2003Assignee: Tosoh CorporationInventors: Ryuichi Horie, Takahiko Ishiguro, Takumi Tokunaga, Takashi Yamamoto, Juichi Saitoh, Toshitaka Taya
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Patent number: 6562955Abstract: An oligonucleotide for detection or amplification of a gene selected from the group consisting of Vibrio parahaemolyticus thermostable direct hemolysin-related hemolysin genes (trh1 and trh2) and Vibrio parahaemolyticus thermostable direct hemolysin gene (tdh2) or RNA derived therefrom is provided. Further, method for detecting trh1, trh2 or tdh2 using said oligonucleotide is provided.Type: GrantFiled: March 15, 2001Date of Patent: May 13, 2003Assignee: Tosoh CorporationInventors: Tetsuya Ishizuka, Takahiko Ishiguro, Juichi Saitoh
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Patent number: 6541205Abstract: A method for assaying a target nucleic acid, comprising: providing an RNA amplification system comprising producing a double-stranded DNA using, as a template, a target RNA containing a specific nucleotide sequence in a sample, said double-stranded DNA having a promoter sequence and being capable of transcribing an RNA comprising the specific nucleotide sequence or a sequence complementary to the specific nucleotide sequence, producing an RNA transcription product comprising the specific nucleotide sequence or a sequence complementary to the specific nucleotide sequence in the presence of an RNA polymerase, and producing the double-stranded DNA using the RNA transcription product as a template, in the presence of a probe labeled with an intercalating fluorochrome having a sequence complementary to the RNA transcription product; measuring the fluorescence intensity in the RNA amplification system with time; calculating a time when the fluorescence intensity satisfies a prescribed criterion based on the measureType: GrantFiled: May 23, 2000Date of Patent: April 1, 2003Assignee: Tosoh CorporationInventors: Akihiro Yokoyama, Takahiko Ishiguro, Juichi Saitoh
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Publication number: 20030026705Abstract: The present invention provides a simple liquid transportation method without requiring high electric voltage or special external equipment, and a method for transporting liquid based on this transportation method. The liquid transportation method comprises a step of introducing a magnetic fluid and a liquid to be transported into a conduit or a chamber connected thereto so that the magnetic fluid and the liquid to be transported are in contact with each other directly or indirectly via a medium, a step of moving the magnetic fluid by applying a magnetic field, and a step of moving above-mentioned liquid to be transported by letting it follow the movement of the magnetic fluid. The present invention also provides a simple transportation method of a specific amount of liquid without requiring high voltage or incorporation of specific actuator components such as valves.Type: ApplicationFiled: June 20, 2002Publication date: February 6, 2003Applicant: TOSOH CORPORATIONInventors: Takahiko Ishiguro, Juichi Saitoh, Yoshifumi Kurihara
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Publication number: 20020155619Abstract: A method for detecting fluorescence from a liquid sample housed in a transparent or translucent sample container includes providing a sample container in a sample holder, which is opaque except for a sample container introduction opening and incoming and outgoing openings for excitation light; layering a liquid sample and a shielding liquid unmixable therewith to prevent external light from entering through the sample container introduction opening; introducing excitation light from such a direction that the excitation light can irradiate the liquid sample before irradiating the shielding liquid; and detecting fluorescence, which emits in a direction of avoiding absorption by the shielding liquid.Type: ApplicationFiled: April 22, 2002Publication date: October 24, 2002Applicant: TOSOH CORPORATIONInventors: Yoshifumi Kurihara, Toshinori Hayashi, Juichi Saitoh, Takahiko Ishiguro
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Publication number: 20010053518Abstract: A simple and accurate method for assay of a single-stranded RNA containing a specific nucleic acids sequence in a sample at almost constant temperature by using at least the following reagents (A) to (I), which comprises a step of adding the reagents (A) to (I) one by one (in any order), in combinations of at least two or all at once andType: ApplicationFiled: July 1, 1999Publication date: December 20, 2001Inventors: TAKAHIKO ISHIGURO, JUICHI SAITOH, TETSUYA ISHIZUKA
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Publication number: 20010031471Abstract: An oligonucleotide for detection or amplification of a gene selected from the group consisting of Vibrio parahaemolyticus thermostable direct hemolysin-related hemolysin genes (trh1 and trh2) and Vibrio parahaemolyticus thermostable direct hemolysin gene (tdh2) or RNA derived therefrom is provided. Further, method for detecting trh1, trh2 or tdh2 using said oligonucleotide is provided.Type: ApplicationFiled: March 15, 2001Publication date: October 18, 2001Inventors: Tetsuya Ishizuka, Takahiko Ishiguro, Juichi Saitoh
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Patent number: 6228656Abstract: A method of specifically cleaving a double-stranded DNA (a target nucleic acid) at a specific nucleic acid sequence, which comprises irradiating a solution containing at least the target nucleic acid, a nucleic acid probe (a single-stranded oligonucleotide) linked to an intercalater and spermine with light with an absorption wavelength of the intercalater.Type: GrantFiled: August 30, 1999Date of Patent: May 8, 2001Assignee: Tosoh CorporationInventors: Takahiko Ishiguro, Masami Otsuka, Juichi Saitoh, Teruhiko Inoue, Yukio Sugiura
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Patent number: 6063572Abstract: A method of assay of a specific nucleic acid anticipated in a sample, which comprises:a DNA producing step which involves production of a double-stranded DNA having a promoter sequence for an RNA polymerase and the nucleic sequence of the specific nucleic acid (the specific nucleic acid sequence) downstream from the promoter sequence by using the specific nucleic acid in the sample as a template, andan RNA producing and measuring step which involves production of a single-stranded RNA having the specific nucleic acid sequence by the RNA polymerase and measurement of the single-stranded RNA,wherein the RNA producing and measuring step is initiated by adding at least the RNA polymerase, ribonucleoside triphosphates and a probe which is labeled with a fluorescent intercalative dye and is complementary to the single-stranded RNA to the reaction solution after the DNA producing step, involves measurement of the fluorescence intensity of the reaction solution, and is carried out at a constant temperature, and doesType: GrantFiled: January 23, 1998Date of Patent: May 16, 2000Assignee: Tosoh CorporationInventors: Takahiko Ishiguro, Juichi Saitoh
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Patent number: 6030115Abstract: A method of measuring a melting temperature of a nucleic acid, which comprises a step of monitoring the fluorescent intensity of a mixture of a sample and a probe which is labeled with a fluorescent intercalative dye and contains a base sequence complementary to a specific nucleic acid in the sample, while varying the temperature of the mixture.Type: GrantFiled: June 2, 1998Date of Patent: February 29, 2000Assignee: Tosoh CorporationInventors: Takahiko Ishiguro, Juichi Saitoh