Abstract: Multiplexed affinity purification and thermal dissociation prior to biochip hybridization simplifies uncharacterized sample admixtures, thereby minimizing or eliminating sample interferents, improving hybridization specificity on a microarray detector, and minimizing or eliminating the need for post-hybridization thermal dissociation analysis. An integrated thermo-affinity sample preparation sub-circuit for sample purification and enrichment is described that is consistent with a field-portable form factor and analytical processes. Thermo-affinity sample preparation on model admixtures of varying complexity was efficacious.

Abstract: New modifiers were synthesized for incorporation of a methacrylic function in 3?-, 5?- and internal positions of oligonucleotides during solid phase synthesis. A modifier was used for synthesis of 5?-methacrylated oligonucleotides for preparation of microarrays by a co-polymerization method.

Abstract: Methods and compositions provide biological microarrays with enhanced fluorescent and luminescent signals by providing refraction index variations within a porous composition used to prepare the microarrays.

Abstract: Multiplexed affinity purification and thermal dissociation prior to biochip hybridization simplifies uncharacterized sample admixtures, thereby minimizing or eliminating sample interferents, improving hybridization specificity on a microarray detector, and minimizing or eliminating the need for post-hybridization thermal dissociation analysis. An integrated thermo-affinity sample preparation sub-circuit for sample purification and enrichment is described that is consistent with a field-portable form factor and analytical processes. Thermo-affinity sample preparation on model admixtures of varying complexity was efficacious.