Abstract: The present concerns systems, methods and computer program products for promoting the mindful consumption of a resource. A method for promoting the mindful consumption of a resource in accordance with one embodiment comprises interacting with a device to enter an initial state, receiving data indicative of a magnitude and direction of acceleration of the device and determining if the received data exceeds a threshold. If the received data exceeds the threshold, the device transitions to a secondary state, causing initiation of a timer. Upon expiration of the timer, the device transitions back to the initial state. According to one embodiment, transitioning from the initial state to the secondary state comprises darkening a first LED, e.g., a green LED, and illuminating a second LED, e.g., a red LED. Likewise transitioning from the secondary state to the initial state may comprise darkening the second LED and illuminating the first LED.

Abstract: The present concerns systems, methods and computer program products for promoting the mindful consumption of a resource. A method for promoting the mindful consumption of a resource in accordance with one embodiment comprises interacting with a device to enter an initial state, receiving data indicative of a magnitude and direction of acceleration of the device and determining if the received data exceeds a threshold. If the received data exceeds the threshold, the device transitions to a secondary state, causing initiation of a timer. Upon expiration of the timer, the device transitions back to the initial state. According to one embodiment, transitioning from the initial state to the secondary state comprises darkening a first LED, e.g., a green LED, and illuminating a second LED, e.g., a red LED. Likewise transitioning from the secondary state to the initial state may comprise darkening the second LED and illuminating the first LED.

Abstract: The invention provides eukaryotic unicellular algae engineered to express a nucleosome alteration protein fused to a protein with affinity to the DNA binding site acting in coordination. An example is a LexA-p300 fusion protein, where the p300 is derived from Chlamydomonas. The LexA binding domain guides the p300 to the binding site and the p300 loosens the nucleosome structure by acetylating histones within proximity of the transgene, thus remodeling the local chromatin structure to allow for high-level expression.

Abstract: The invention provides eukaryotic unicellular algae engineered to express a nucleosome alteration protein fused to a protein with affinity to the DNA binding site acting in coordination. An example is a LexA-p300 fusion protein, where the p300 is derived from Chlamydomonas. The LexA binding domain guides the p300 to the binding site and the p300 loosens the nucleosome structure by acetylating histones within proximity of the transgene, thus remodeling the local chromatin structure to allow for high-level expression.

Abstract: The invention provides a system of enhancing the expression of transgenes in algae. Transgenes are engineered to have a binding site for certain proteins in proximity to their promoter, for example a LexA binding site. The algae is also engineered to express a nucleosome alteration protein fused to a protein with affinity to the DNA binding site acting in coordination. An example is a LexA-p300 fusion protein, where the p300 is derived from Chlamydomonas. The LexA binding domain guides the p300 to the binding site and the p300 loosens the nucleosome structure by acetylating histones within proximity of the transgene, thus remodeling the local chromatin structure to allow for high-level expression.

Abstract: The invention provides a system of enhancing the expression of transgenes in algae. Transgenes are engineered to have a binding site for certain proteins in proximity to their promoter, for example a LexA binding site. The algae is also engineered to express a nucleosome alteration protein fused to a protein with affinity to the DNA binding site acting in coordination. An example is a LexA-p300 fusion protein, where the p300 is derived from Chlamydomonas. The LexA binding domain guides the p300 to the binding site and the p300 loosens the nucleosome structure by acetylating histones within proximity of the transgene, thus remodeling the local chromatin structure to allow for high-level expression.