Patents by Inventor Jumpei KIYOKAWA

Jumpei KIYOKAWA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for inducing differentiation of pluripotent stem cells into hepatocytes
    Patent number: 11692173
    Abstract: The present invention provides, in order to prepare matured hepatocytes analogous in various points to primary hepatocytes, a method for preparing hepatocytes or cells that can be differentiated into hepatocytes from pluripotent stem cells, comprising the steps of: (1) culturing the pluripotent stem cells in a medium containing an activator of an activin receptor-like kinase-4,7; (2) culturing the cells obtained in the step (1) in a medium containing a bone morphogenetic factor and a fibroblast growth factor; (3) culturing the cells obtained in the step (2) in a medium containing an activator of a hepatocyte growth factor receptor and an activator of an oncostatin M receptor; and (4) culturing the cells obtained in the step (3) to obtain hepatocytes or cells that can be differentiated into hepatocytes, wherein in at least one of the steps (2), (3) and (4), cells are cultured on a high-density collagen gel membrane.
    Type: Grant
    Filed: October 9, 2018
    Date of Patent: July 4, 2023
    Assignees: TOKYO INSTITUTE OF TECHNOLOGY, KANTO KAGAKU KABUSHIKI KAISHA, CHUGAI SEIYAKU KABUSHIKI KAISHA
    Inventors: Shoen Kume, Nobuaki Shiraki, Hiroyuki Yamaguchi, Tomoaki Inoue, Toshito Nakagawa, Jumpei Kiyokawa
  • METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO HEPATOCYTES
    Publication number: 20210207085
    Abstract: The present invention provides, in order to prepare matured hepatocytes analogous in various points to primary hepatocytes, a method for preparing hepatocytes or cells that can be differentiated into hepatocytes from pluripotent stem cells, comprising the steps of: (1) culturing the pluripotent stem cells in a medium containing an activator of an activin receptor-like kinase-4,7; (2) culturing the cells obtained in the step (1) in a medium containing a bone morphogenetic factor and a fibroblast growth factor; (3) culturing the cells obtained in the step (2) in a medium containing an activator of a hepatocyte growth factor receptor and an activator of an oncostatin M receptor; and (4) culturing the cells obtained in the step (3) to obtain hepatocytes or cells that can be differentiated into hepatocytes, wherein in at least one of the steps (2), (3) and (4), cells are cultured on a high-density collagen gel membrane.
    Type: Application
    Filed: October 9, 2018
    Publication date: July 8, 2021
    Applicants: TOKYO INSTITUTE OF TECHNOLOGY, KANTO KAGAKU KABUSHIKI KAISHA, CHUGAI SEIYAKU KABUSHIKI KAISHA
    Inventors: Shoen KUME, Nobuaki SHIRAKI, Hiroyuki YAMAGUCHI, Tomoaki INOUE, Toshito NAKAGAWA, Jumpei KIYOKAWA