Abstract: A composition useful for separating lipoproteins, such as high density lipoprotein (HDL), low density lipoprotein (LDL), very low density lipoprotein (VLDL) and modified lipoproteins with a high degree of accuracy by ion-exchange based procedures. The composition comprises an organic particle having a hydrophilic surface onto which ion-exchange groups are introduced and which is formulated so as to provide a high enough degree of hydrophilicity to prevent irreversible absorption of lipoproteins, but which is not so high as to block the pores of the ion exchange particles and prevent adequate retention and separation of lipoproteins. A method for making a composition for separating lipoproteins comprising seed polymerization of organic particles, hydrophilic treatment of the organic particles by introducing 0.

Abstract: An ion exchanger for lipoproteins separation, which is an ion exchanger obtained by introducing an ion exchange group into surface hydrophilic-treated porous organic polymer particles (D) prepared by subjecting the surface of porous organic polymer particles (A) to hydrophilic treatment, wherein said hydrophilic treatment is carried out by allowing the porous organic polymer particles (A) to undergo addition reaction with a compound (B) having a cyclic other group or/and a hydrophilic group and a polymerizable unsaturated double bond and then to undergo copolymerization with a polymerizable vinyl monomer (C) having a cyclic ether group or/and a hydrophilic group, and wherein the ion exchange capacity is 0.15 milliequivalent or less per 1 g of dry ion exchanger, introduced amount of the compound (B) is from 0.

Abstract: An optical isomer separating agent comprises a stationary phase in which the molecular structure of ovomucoid fixed to a carrier is partly modified, or a stationary phase in which ovomucoid having the molecule partly modified is fixed to a carrier is disclosed. A process for producing an optical isomer separating agent comprises the steps of immobilizing ovomucoid on a carrier and modifying the ovomucoid is disclosed. Furthermore a process for producing an optical isomer separating agent comprises the steps of modifying ovomucoid and immobilizing the modified ovomucoid on a carrier is disclosed.

Abstract: An ovoglycoprotein has a molecular weight of 30,000 daltons which is determined by a matrix-assisted laser desorption ionization time-of-flight type mass spectrometer, an amino acid sequence of 15 residues from the N-terminal represented by Thr-Glu-Ser-Pro-Xaa-Ser-Ala-Pro-Leu-Val-Pro-Ala-Asp-Met-Asp (wherein Xaa represents a cysteine residue or an amino acid residue having a sugar chain linked thereto) (SEQ ID NO:1) and a sugar content of about 25% by weight and free of trypsin-inhibitory activity and a resolution agent for chromatography comprises a fixed phase which comprises a carrier and the foregoing ovoglycoprotein linked to the carrier. The agent for resolving optical isomers is not expensive, and is stable and highly resistant to deterioration by organic solvents, excellent in liquid-flow through properties, has a high sample-loading rate and permits efficient resolution of optical isomers of a chiral compound.