Patents by Inventor Jurate Bitinaite
Jurate Bitinaite has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220380738Abstract: The present disclosure relates, according to some embodiments, to compositions, methods, systems, and kits for programmable endonucleolytic cleavage of DNA (e.g., ds DNA). For example, the in vitro activity of an Argonaute (e.g., a mesophilic Argonaute CbAgo from Clostridium butyricum) may be synchronized with DNA strand unwinding activity of a helicase (e.g., a nuclease deficient RecBexo-C DNA helicase from E. coli) for a rapid and efficient cleavage of double-stranded DNA targets. Enzymatic properties of CbAgo and different aspects of ds DNA cleavage were thoroughly explored by adapting high-throughput capillary electrophoreses technique for monitoring CbAgo cleavage activity in concurrence with RecBexo-C. The present disclosure shows that in the presence of RecBexo-C, CbAgo can be programmed with guides to cleave any site of interest localized at up to 10 kb distance from the end of linear ds DNA at 37° C. temperature.Type: ApplicationFiled: June 1, 2021Publication date: December 1, 2022Applicant: New England Biolabs, Inc.Inventors: Jurate Bitinaite, Rita Vaiskunaite, Vladimir Potapov, Nathan Tanner
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Compositions and Methods for the Transfer of a Hexosamine to a Modified Nucleotide in a Nucleic Acid
Publication number: 20160046981Abstract: Nucleic acids comprising ?-glucosaminyloxy-5-methylcytosine; compositions, kits and methods of producing the nucleic acids using a glycosyltransferase; and methods of using the nucleic acids are described.Type: ApplicationFiled: October 29, 2015Publication date: February 18, 2016Applicant: NEW ENGLAND BIOLABS, INC.Inventors: Ivan R. Correa, JR., Nan Dai, Jurate Bitinaite, Sriharsa Pradhan, Hang-Gyeong Chin -
Compositions and methods for the transfer of a hexosamine to a modified nucleotide in a nucleic acid
Patent number: 9200260Abstract: Nucleic acids comprising ?-glucosaminyloxy-5-methylcytosine; compositions, kits and methods of producing the nucleic acids using a glycosyltransferase; and methods of using the nucleic acids are described.Type: GrantFiled: March 14, 2013Date of Patent: December 1, 2015Assignee: New England Biolabs, Inc.Inventors: Ivan R. Correa, Jr., Nan Dai, Jurate Bitinaite, Sriharsa Pradhan, Hang-Gyeong Chin -
Publication number: 20150240310Abstract: Methods and compositions are described for detecting hydroxymethylated nucleotides (hmNs) in a polynucleotide preparation with a view to mapping the location of hmNs in a genome, quantifying the occurrence of hmNs at selected loci and correlating the occurrence of hmNs with gene expression and phenotypic traits. Embodiments describe the use of modifying enzymes together with site-specific endonucleases to detect the hmNs.Type: ApplicationFiled: April 14, 2015Publication date: August 27, 2015Applicant: NEW ENGLAND BIOLABS, INC.Inventors: Jurate Bitinaite, Romualdas Vaisvila, Sriharsa Pradhan, Yu Zheng, Richard J. Roberts, Hang-Gyeong Chin, Devora Cohen-Karni, Christopher Noren, Elisabeth A. Raleigh, Geoffrey Wilson
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Patent number: 9034597Abstract: Methods and compositions are described for detecting hydroxymethylated nucleotides (hmNs) in a polynucleotide preparation with a view to mapping the location of hmNs in a genome, quantifying the occurrence of hmNs at selected loci and correlating the occurrence of hmNs with gene expression and phenotypic traits. Embodiments describe the use of modifying enzymes together with site-specific endonucleases to detect the hmNs.Type: GrantFiled: August 25, 2010Date of Patent: May 19, 2015Assignee: New England Biolabs, Inc.Inventors: Jurate Bitinaite, Romualdas Vaisvila, Sriharsa Pradhan, Yu Zheng, Richard J. Roberts, Devora Cohen-Karni, Christopher Noren, Elisabeth A. Raleigh, Geoffrey Wilson, Hang-Gyeong Chin
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Compositions and Methods for the Transfer of a Hexosamine to a Modified Nucleotide in a Nucleic Acid
Publication number: 20140127677Abstract: Nucleic acids comprising ?-glucosaminyloxy-5-methylcytosine; compositions, kits and methods of producing the nucleic acids using a glycosyltransferase; and methods of using the nucleic acids are described.Type: ApplicationFiled: March 14, 2013Publication date: May 8, 2014Applicant: NEW ENGLAND BIOLABS, INC.Inventors: Ivan R. Correa, JR., Nan Dai, Jurate Bitinaite, Sriharsa Pradhan, Hang-Gyeong Chin -
Patent number: 8440401Abstract: single-stranded extension having a desired length and sequence composition. Methods for forming single-stranded extensions include: the use of a cassette containing at least one nicking site and at least one restriction site at a predetermined distance from each other and in a predetermined orientation; or primer-dependent amplification which introduces into a polynucleotide molecule, a modified nucleotide which is excised to create a nick using a nicking agent. The methods and compositions provided can be used to manipulate a DNA sequence including introducing site specific mutations into a polynucleotide molecule and for cloning any polynucleotide molecule or set of joined polynucleotide molecules in a recipient molecule such as a vector of choice.Type: GrantFiled: March 21, 2011Date of Patent: May 14, 2013Assignee: New England Biolabs, Inc.Inventor: Jurate Bitinaite
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Publication number: 20120156677Abstract: Methods and compositions are described for detecting hydroxymethylated nucleotides (hmNs) in a polynucleotide preparation with a view to mapping the location of hmNs in a genome, quantifying the occurrence of hmNs at selected loci and correlating the occurrence of hmNs with gene expression and phenotypic traits. Embodiments describe the use of modifying enzymes together with site-specific endonucleases to detect the hmNs.Type: ApplicationFiled: August 25, 2010Publication date: June 21, 2012Applicant: NEW ENGLAND BIOLABS, INC.Inventors: Jurate Bitinaite, Romualdas Vaisvila, Sriharsa Pradhan, Yu Zheng, Richard J. Roberts, Devora Cohen-Karni, Christopher Noren, Elisabeth A. Raleigh, Geoffrey Wilson, Hang-Gyeong Chin
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Publication number: 20110262973Abstract: single-stranded extension having a desired length and sequence composition. Methods for forming single-stranded extensions include: the use of a cassette containing at least one nicking site and at least one restriction site at a predetermined distance from each other and in a predetermined orientation; or primer-dependent amplification which introduces into a polynucleotide molecule, a modified nucleotide which is excised to create a nick using a nicking agent. The methods and compositions provided can be used to manipulate a DNA sequence including introducing site specific mutations into a polynucleotide molecule and for cloning any polynucleotide molecule or set of joined polynucleotide molecules in a recipient molecule such as a vector of choice.Type: ApplicationFiled: March 21, 2011Publication date: October 27, 2011Applicant: NEW ENGLAND BIOLABS, INC.Inventor: Jurate Bitinaite
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Publication number: 20090042258Abstract: Methods and compositions are provided for generating a single-stranded extension on a polynucleotide molecule, the single-stranded extension having a desired length and sequence composition. Methods for forming single-stranded extensions include: the use of a cassette containing at least one nicking site and at least one restriction site at a predetermined distance from each other and in a predetermined orientation; or primer-dependent amplification which introduces into a polynucleotide molecule, a modified nucleotide which is excised to create a nick using a nicking agent. The methods and compositions provided can be used to manipulate a DNA sequence including introducing site specific mutations into a polynucleotide molecule and for cloning any polynucleotide molecule or set of joined polynucleotide molecules in a recipient molecule such as a vector of choice.Type: ApplicationFiled: August 15, 2008Publication date: February 12, 2009Applicant: NEW ENGLAND BIOLABS, INC.Inventor: Jurate Bitinaite
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Patent number: 7435572Abstract: Methods and compositions are provided for generating a single-stranded extension on a polynucleotide molecule, the single-stranded extension having a desired length and sequence composition. Methods for forming single-stranded extensions include: the use of a cassette containing at least one nicking site and at least one restriction site at a predetermined distance from each other and in a predetermined orientation; or primer-dependent amplification which introduces into a polynucleotide molecule, a modified nucleotide which is excised to create a nick using a nicking agent. The methods and compositions provided can be used to manipulate a DNA sequence including introducing site specific mutations into a polynucleotide molecule and for cloning any polynucleotide molecule or set of joined polynucleotide molecules in a recipient molecule such as a vector of choice.Type: GrantFiled: April 4, 2003Date of Patent: October 14, 2008Assignee: New England Biolabs, Inc.Inventor: Jurate Bitinaite
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Publication number: 20030194736Abstract: Methods and compositions are provided for generating a single-stranded extension on a polynucleotide molecule, the single-stranded extension having a desired length and sequence composition. Methods for forming single-stranded extensions include: the use of a cassette containing at least one nicking site and at least one restriction site at a predetermined distance from each other and in a predetermined orientation; or primer-dependent amplification which introduces into a polynucleotide molecule, a modified nucleotide which is excised to create a nick using a nicking agent. The methods and compositions provided can be used to manipulate a DNA sequence including introducing site specific mutations into a polynucleotide molecule and for cloning any polynucleotide molecule or set of joined polynucleotide molecules in a recipient molecule such as a vector of choice.Type: ApplicationFiled: April 4, 2003Publication date: October 16, 2003Inventor: Jurate Bitinaite