Abstract: There is provided a recombinant bacterium comprising at least one overexpressed acyl-ACP thioesterase gene, and wherein at least one gene from the tricarboxylic acid cycle or glycolysis or both is inactivated. There is also provided a method for producing fatty acids, said method comprising culturing bacteria comprising at least one overexpressed acyl-ACP thioesterase gene in a growth medium in a container having walls; allowing said bacteria to secrete fatty acids; and collecting said fatty acids. Acid supplementation is also shown to increase productivity.

Abstract: The disclosure relates to biological methods of making a hydrocarbon feedstock wherein one-carbon substrates are converted into useful chemicals and fuels. Particularly, genetically engineered bacteria are used to make C4-C10 fatty acids or derivatives from one-carbon substrates such as methanol and carbon dioxide.

Abstract: Methods of improving fatty acid production in bacteria, yeast, algae, and various other microbes are presented, by either supplementing the medium with millimolar amounts of magnesium, by overexpressing a magnesium transporter, or both.

Abstract: Systems, methods and microbes that allow the biological production of hydroxy fatty acids and dicarboxylic fatty acids are provided. Specifically, hydroxy fatty acids and dicarboxylic fatty acids are produced by microbes that have been engineered to overexpress acyl ACP thioesterase plus an alkane degration pathway, such as AlkBGT or AlkJH These can be in separate microbes or the same microbe, and separate microbes can be co-cultured or sequentially cultured. Continuously fed systems transferring secreted fats from one microbial culture to another can also be used.

Abstract: Bacteria optimized to produce succinate and other feedstocks by growing on low cost carbon sources, such as sucrose.

Abstract: A method of increasing cellular NADPH levels by expressing one or more genes that encode an enzyme that causes the production of NADPH. The system is combined with other enzymes that require NADPH, thus improving the overall yield of the desired product.

Abstract: Methods of using crude glycerol to make fatty acids are provided, as well as integrated methods of converting glycerol waste from biodiesel production into more biodiesel. Bacteria and other microbes engineered to produce free fatty acids from glycerol are also provided.

Abstract: The present invention discloses a process for increasing the production of free fatty acids at high yield (close to maximum theoretical yield), with various fatty acid compositions and various percentage of fatty acids accumulated intracellularly. This invention will enable the efficient production of other products derived from free fatty acids and/or products that can be branched out from the fatty acid synthesis pathways.

Abstract: The disclosure relates to a metabolic transistor in bacteria where a competitive pathway is introduced to compete with a product pathway for available carbon so as to control the carbon flux in the bacteria.

Abstract: Improved bacteria for making succinate and other 4 carbon dicarboxylates from the Krebs cycle have modifications to reduce acetate, lactate, EtOH and formate, as well as turn on the glyoxylate shunt, produce more NADH and overexpress In one embodiment, the bacteria are ?adhE?ldhA?iclR?ack-pta plus PYC+ and NAD+-dependant FDH+.

Abstract: The present invention relates to an engineered bacteria for producing short chain fatty acid with the overexpression of a long chain (>C12) acyl-ACP thioesterases (long-TE) and a short chain (?C12) acyl-ACP thioesterases (short-TE).

Abstract: Engineered bacteria that produce a predominance of odd-chain free fatty acids.

Abstract: This invention is metabolically engineer bacterial strains that provide increased intracellular NADPH availability for the purpose of increasing the yield and productivity of NADPH-dependent compounds. In the invention, native NAD-dependent GAPDH is replaced with NADP-dependent GAPDH plus overexpressed NADK. Uses for the bacteria are also provided.

Abstract: There is provided a recombinant bacterium comprising at least one overexpressed acyl-ACP thioesterase gene, and wherein at least one gene from the tricarboxylic acid cycle or glycolysis or both is inactivated. There is also provided a method for producing fatty acids, said method comprising culturing bacteria comprising at least one overexpressed acyl-ACP thioesterase gene in a growth medium in a container having walls; allowing said bacteria to secrete fatty acids; and collecting said fatty acids. Acid supplementation is also shown to increase productivity.

Abstract: Improved bacteria for making succinate and other 4 carbon dicarboxylates from the Krebs cycle have modifications to reduce acetate, lactate, EtOH and formate, as well as turn on the glyoxylate shunt, produce more NADH and overexpress In one embodiment, the bacteria are ?adhE?ldhA?iclR?ack-pta plus PYC+ and NAD+-dependant FDH+.

Abstract: A new culture method for producing high levels of a metabolite, such as succinic acid uses oxygen rich culture without pH adjustment to increase the biomass, acclimation in under oxygen lean conditions having <5% partial pressure of oxygen, and the production of high levels of succinate under oxygen deprived conditions. The method can be performed in a single reactor, and is amenable to efficient scale up.

Abstract: Bacteria optimized to produce succinate and other feedstocks by growing on low cost carbon sources, such as sucrose.

Abstract: A method of producing biological products using bacteria with an inactivated ptsHI and wild type err and no added glucose transport activity and which consumes nearly all glucose in the media is described. The ?ptsHI bacteria produce large quantities of recombinant protein without producing significant amounts of acetate. The bacteria grow well on standard LB broth without additional supplementation.

Abstract: New and useful compounds C. roseus cultured hairy roots are provided, by Isolating flavone glycosides together with a pharmaceutically acceptable carrier. This is accomplished by immersing powdered the hairy roots in methanol to produce an extract, concentrating the extract, resuspending the extract in water, extracting the compound with organic solvent, and isolating the compound from the organic solvent.

Abstract: The invention relates to the synthesis of polyketides in Actinomycetes by overexpression of pantothenate kinase and supplementation with pantothenic acid. This results in increasing in vivo CoA production and thereby drives increased production of secondary metabolites, such as polyketides.