Patents by Inventor Kan HANDA

Kan HANDA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Human induced pluripotent stem cells for high efficiency genetic engineering
    Patent number: 11866733
    Abstract: Methods are disclosed herein for efficiently generating human induced pluripotent stem cells (iPSC) containing a nucleic acid including a doxycycline promoter operably linked to a nucleic acid encoding Cas9. These methods include transfecting a human somatic cell with a nucleic acid molecule comprising a doxycycline promoter operably linked to a nucleic acid encoding a Cas9, and constitutive promoter operably linked to a tetracycline responsive element and inducing the somatic cell to form an iPSC, thereby producing an iPSC that can undergo CRISPR/Cas9-mediated recombination at a high efficiency. The human iPSC, or a cell differentiated therefrom, is cultured in the presence of doxycycline to induce expression of the Cas9. These cells can then be used to target in any gene of interest by introducing nucleic acids encoding sgRNAs. Induced pluripotent stem cells produced by these methods are also disclosed.
    Type: Grant
    Filed: July 31, 2017
    Date of Patent: January 9, 2024
    Assignee: University of Pittsburgh—Of the Commonwealth System of Higher Education
    Inventors: Alejandro Soto-Gutierrez, Alexandra Sylvie Collin de l'Hortet, Kan Handa, Jorge Guzman Lepe, Yang Wang, Kazuki Takeishi, Ira Jacob Fox
  • Methods of engineering human induced pluripotent stem cells to produce liver tissue
    Patent number: 11530388
    Abstract: Methods are disclosed herein for producing human hepatocytes from human induced pluripotent stem cells. Also provided are transgenic rats for the expansion of human hepatocytes, such as those produced using the methods disclosed herein.
    Type: Grant
    Filed: February 13, 2018
    Date of Patent: December 20, 2022
    Assignee: University of Pittsburgh—Of the Commonwealth System of Higher Education
    Inventors: Alejandro Soto-Gutierrez, Tomoji Mashimo, Alexandra Sylvie Collin de l'Hortet, Eduardo Cervantes Alvarez, Jorge Guzman Lepe, Kan Handa, Kazuki Takeishi, Yang Wang, Branimir Popovic
  • METHODS OF ENGINEERING HUMAN INDUCED PLURIPOTENT STEM CELLS TO PRODUCE LIVER TISSUE
    Publication number: 20190376029
    Abstract: Methods are disclosed herein for producing human hepatocytes from human induced pluripotent stem cells. Also provided are transgenic rats for the expansion of human hepatocytes, such as those produced using the methods disclosed herein.
    Type: Application
    Filed: February 13, 2018
    Publication date: December 12, 2019
    Applicant: University of Pittsburgh - Of the Commonwealth System of Higher Education
    Inventors: Alejandro Soto-Gutierrez, Tomoji Mashimo, Alexandra Sylvie Collin de l'Hortet, Eduardo Cervantes Alvarez, Jorge Guzman Lepe, Kan Handa, Kazuki Takeishi, Yang Wang, Branimir Popovic
  • HUMAN INDUCED PLURIPOTENT STEM CELLS FOR HIGH EFFICIENCY GENETIC ENGINEERING
    Publication number: 20190185819
    Abstract: Methods are disclosed herein for efficiently generating human induced pluripotent stem cells (iPSC) containing a nucleic acid including a doxycycline promoter operably linked to a nucleic acid encoding Cas9. These methods include transfecting a human somatic cell with a nucleic acid molecule comprising a doxycycline promoter operably linked to a nucleic acid encoding a Cas9, and constitutive promoter operably linked to a tetracycline responsive element and inducing the somatic cell to form an iPSC, thereby producing an iPSC that can undergo CRISPR/Cas9-mediated recombination at a high efficiency. The human iPSC, or a cell differentiated therefrom, is cultured in the presence of doxycycline to induce expression of the Cas9. These cells can then be used to target in any gene of interest by introducing nucleic acids encoding sgRNAs. Induced pluripotent stem cells produced by these methods are also disclosed.
    Type: Application
    Filed: July 31, 2017
    Publication date: June 20, 2019
    Applicant: University of Pittsburgh - Of the Commonwealth System of Higher Education
    Inventors: Alejandro Soto-Gutierrez, Alexandra Sylvie Collin de l'Hortet, Kan Handa, Jorge Guzman Lepe, Yang Wang, Kazuki Takeishi, Ira Jacob Fox
  • Method of Preparing Artificial Organs, and Related Compositions
    Publication number: 20170049941
    Abstract: Provided herein are methods or making and using whole or partial organ ECM structures comprising an anticoagulant. Also provided are organ structures prepared according to those methods.
    Type: Application
    Filed: April 29, 2015
    Publication date: February 23, 2017
    Inventors: Alejandro SOTO-GUTIERREZ, Kentaro MATSUBARA, Ken FUKUMITSU, Kan HANDA, Jorge Guzman LEPE, William R. WAGNER, Sang Ho YE, Hiroshi YAGI, Yuko KITAGAWA