Abstract: Site-specific modifications of proteins are desirable in biotechnological applications such as biopharmaceuticals, immunotherapy, vaccines, and are useful in chemical biology. Gluconoylation is a non-enzymatic, covalent, post-translational modification commonly observed on N-terminal His-Tags bearing proteins. We synthesized glucono-1,5-lactone derivatives, including azido variants for selective acylation. High yield acylation is achieved by simply mixing derivatives with target protein amidst diverse conditions of temperatures, aqueous buffers, excipients, or complex cell lysate.

Abstract: A device for the supply or discharge of medium into or out of a culture vessel comprises a housing on which a first aperture and a plurality of second apertures are arranged. The first aperture and the second apertures have fluid-conducting connection to one another in a manner to supply a medium from the first aperture via the second apertures into the interior of the culture vessel or to discharge same in reversed direction therefrom, when the device is attached on the culture vessel. The plurality of second apertures are configured to generate a plurality of medium sub-streams arranged in parallel to one another of the medium that is to be supplied or that is to be discharged. Further disclosed are a culture vessel and a method of cultivating microbiological systems.

Abstract: A stimulation electrode for a stimulation device for stimulating organs in the case of spinal paraplegia, more particularly in the case of detrusor sphincter dyssynergia, including an electrode shaft, at one end of which an electrode tip is arranged for emitting a stimulation signal and at the other end of which a connection region is provided for a signal line, and also at least one fixing mechanism for fixing the position of the electrode shaft in the surrounding tissue along the longitudinal axis of the electrode shaft, the fixing mechanism having at least one holding member preventing a withdrawal and at least one holding member preventing an advance.

Abstract: The invention relates to a method of purifying PEGylated proteins by removing impurities from samples containing PEGylated proteins, in particular, but not exclusively vitamin K-dependent blood coagulation factors such as Factor IX (FIX), to proteins purified by said method and to the use of said purified proteins in therapy, in particular but not exclusively, for the treatment of diseases alleviated by blood coagulation factors such as the prophylactic treatment of hemophilia.

Abstract: The invention relates to a method of purifying PEGylated proteins by removing impurities from samples containing PEGylated proteins, in particular, but not exclusively vitamin K-dependent blood coagulation factors such as Factor IX (FIX), to proteins purified by said method and to the use of said purified proteins in therapy, in particular but not exclusively, for the treatment of diseases alleviated by blood coagulation factors such as the prophylactic treatment of hemophilia.

Abstract: The invention relates to a stimulation electrode for a stimulation device for stimulating organs in the case of spinal paraplegia, more particularly in the case of detrusor sphincter dyssynergia, including an electrode shaft, at one end of which an electrode tip is arranged for emitting a stimulation signal and at the other end of which a connection region is provided for a signal line, and also at least one fixing mechanism for fixing the position of the electrode shaft in the surrounding tissue along the longitudinal axis of said electrode shaft, fixing mechanism having at least one holding member preventing a withdrawal and at least one holding member preventing an advance.

Abstract: The present invention relates to an apparatus for the manufacture of corrugated pipes from thermoplastic in which mould segment halves, which complement one another in pairs, are guided together in a circuit along a mould path adjacent to an injection head, are moved apart at the end of the mould path and are returned back to its start separately from one another. The apparatus has a mould path device to move the mould segment halves along the mould path and a return conveyor to return the mould segment halves from the end of the mould path back to its start, with the mould path device comprising a machine table having a lower guide and lower drive means for the mould segment halves and a cover frame secured to the machine table having an upper guide and upper drive means for the mould segment halves.

Abstract: The present invention relates to a method and an apparatus for producing a reinforced socket on an extruded plastic tube made of thermoplastic material, preferably a corrugated tube, in which the tube is provided with end sockets at predetermined segment lengths. One end of the plastic tube or of a segment thereof is placed on a support mandrel and together with this support mandrel is rotated about its longitudinal axis. During the rotation, a layer of plastic is extruded onto a pre-formed socket region of the tube and/or onto the support mandrel, forming a reinforced socket formed on the tube.

Abstract: Specific lengths of tube sections have to be severed from a run of spiral seam tubes being produced, in order to facilitate handling and storage. A cutter, with which sections of tubes can be severed cleanly without the formation of burrs from the run of tubes, comprises a carriage that can be slid back and forth on guides at right angle to the axis of the tube and on which carriage are mounted in the cut plane a milling cutter and shears, both of which can be moved on the tube independently of each other and back again with controlled drives.

Abstract: An article of footwear is disclosed, in particular a sports shoe with a sole ensemble and a stability element constructed of a material and configured for the selective support of single parts of the sole ensemble. The stability element includes a base element extending from a rearfoot portion to a forefoot portion of a shoe along the medial or the lateral side of the forefoot portion of the shoe and includes at least one support element. The at least one support element encompasses the sole ensemble upwardly or downwardly or both. Further, the stability element may include a heel support connected to the base element and a second base element for additional lateral support of the shoe.

Abstract: The present invention relates to an apparatus for the manufacture of corrugated pipes from thermoplastic in which mould segment halves, which complement one another in pairs, are guided together in a circuit along a mould path adjacent to an injection head, are moved apart at the end of the mould path and are returned back to its start separately from one another. The apparatus has a mould path device to move the mould segment halves along the mould path and a return conveyor to return the mould segment halves from the end of the mould path back to its start, with the mould path device comprising a machine table having a lower guide and lower drive means for the mould segment halves and a cover frame secured to the machine table having an upper guide and upper drive means for the mould segment halves.

Abstract: An article of footwear is disclosed, in particular a sports shoe with a sole ensemble and a stability element constructed of a material and configured for the selective support of single parts of the sole ensemble. The stability element includes a base element extending from a rearfoot portion to a forefoot portion of a shoe along the medial or the lateral side of the forefoot portion of the shoe and includes at least one support element. The at least one support element encompasses the sole ensemble upwardly or downwardly or both. Further, the stability element may include a heel support connected to the base element and a second base element for additional lateral support of the shoe.

Abstract: An apparatus for the manufacture of corrugated tubing of thermoplastic including a shaping path adjoining the head of an extruder, composed of a guide arranged in a frame structure, along which guide mold halves closed together in pairs as annular molds are advanced in close succession, said mold halves being provided on a lower side thereof with racks, at least one drive pinion arranged in the initial part of the shaping path for meshing with the racks of the mold halves as the same are pushed along the path, and an outlet, following the shaping path, in which the mold halves are separated from one another and are transferred to mutually separate return paths, on which they are shunted back by a conveyor to the inlet.

Abstract: An apparatus for the production of corrugated tubing from thermoplastic synthetic resin including a shaping path following an extrusion head of an extruder and composed of a guide which is arranged in a frame and on which mold halves, fitted together in a pair at an inlet to define annular molds in a compact train as a travelling mold moving with the tube are advanced by at least one drive pinion arranged at an initial part of the shaping path, the pinion being in mesh with racks on the fitted together mold halves. At an exit point following the shaping path, the mold halves are separated from one another and are gripped by gripping arms provided with coupling devices and area shunted back to the inlet of the shaping path. The gripping arms are bearinged by drives in a movable fashion on carriages. The carriages are able to be reciprocated by drives on guides on return paths parallel to the shaping path.

Abstract: A process for the continuous production of an irrigating tube having trickling elements embedded between an inner tube and an outer tube; provides that, after extrusion of an inner tube and subsequent cooling of the tube, the latter is perforated at regular intervals to produce inlet orifices, that the tube is subsequently heated up again, at least in the region of the perforations, and that thereafter a trickling element is pressed into the surface of the inner tube in each case at the heated-up perforation points and is thereby connected to the inner tube. The inner tube and trickling elements are subsequently surrounded by an outer tube, in which outer outlet orifices are made at the points of the trickling elements.

Abstract: In a peeling machine for peeling fruit or vegetables, for example a continuously operating roller peeling machine which comprises motor-driven peeling rollers and a device, driven independently of the peeling rollers, for metered conveyance of the fruit or vegetables to be peeled, through the machine, the quality of the peeled fruit or vegetables and the peeling performance are decisively influenced by controlling the speeds of the conveying device and of the peeling rollers and also controlling the input rate of fruit or vegetables to be peeled. In order to make the control not solely dependent upon the judgement of the operator of the machine, the magnitude of the input quantity of the fruit or vegetables and of water supplied to the machine and also of the water containing peelings discharged from the machine are measured.

Abstract: The present invention provides a method for the determination of peroxidase in the presence of a compound, the enzyme-catalysed oxidation of which with a peroxy compound results in the emission of light, which is measured, wherein 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide of the formula ##STR1## is used as light-emitting compound. The present invention also provides a reagent for the determination of peroxidase, comprising 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide, a system providing hydrogen peroxide and a buffer substance of pH 6 to 9.