Abstract: Methods are provided for producing novel multicellular compositions comprising cancer cells together with pluripotent human stem cells, which are capable of proliferating and differentiating into various normal cell lines and tissue structures. These novel multicellular compositions are useful for investigating the properties of cancer cells in a normal human tissue microenvironment, and for studying interventions that will modulate these properties including devising, testing and screening therapeutic drugs.

Abstract: Methods are provided for producing novel multicellular compositions comprising cancer cells together with pluripotent human stem cells, which are capable of proliferating and differentiating into various normal cell lines and tissue structures. These novel multicellular compositions are useful for investigating the properties of cancer cells in a normal human tissue microenvironment, and for studying interventions that will modulate these properties including devising, testing and screening therapeutic drugs.

Abstract: The present invention discloses insulin producing cells derived from human embryonic stem cells. Methods of generating, enriching, selecting, cloning and isolating populations of insulin-producing embryonic cell lines are disclosed. Insulin production in these cells can be glucose responsive or otherwise regulatable. Advantageously, these insulin producing cells can be stable cell lines derived from stable transfectants of hESC. Methods of using these cells, cell populations or cell lines particularly for cell replacement therapy are also disclosed.