Abstract: Systems, compositions, and methods for target site-specific insertion of a transgene of interest to a subject genome are provided. Systems and methods that facilitate primed reverse transcription (TPRT) mediated by retroelement derived reverse transcriptase (RTs) site-specific transgene insertion are also provided.

Abstract: Improved compositions and methods for using modified non-retroviral reverse transcriptase to perform 3? extension of a nucleic acid, employ non-templated deoxynucleotide addition to a single-stranded nucleic acid and/or synthesis of complementary DNA using non-complementary nucleic acids as primer and template (RNA- or DNA-templated DNA polymerase activity.

Abstract: The invention provides compositions and methods for nucleic acid synthesis, including ordered and continuous complementary DNA (cDNA) synthesis across non-continuous templates using a modified eukaryotic non-long terminal repeat reverse transcriptase (non-LTR RT) protein.

Abstract: A computer system presents information related to drilling operations using a computer display, the method comprising: for each of a plurality of drilling sites, obtaining chemical composition data of hydraulic fluids used in the drilling site, wherein the chemical composition data is obtained from a first data source, for each of the plurality of drilling sites, obtaining well yield data for the drilling site, wherein the well yield data is obtained from a second data source distinct from the first data source, matching the chemical composition data and the well yield data by drilling site, and displaying, on the computer display, a combination of chemical composition and well yield for the plurality of drilling sites. The matching might be based on drill site parameters, such as geographic location and/or geologic characteristics.

Abstract: A computer system presents information related to drilling operations using a computer display, the method comprising: for each of a plurality of drilling sites, obtaining chemical composition data of hydraulic fluids used in the drilling site, wherein the chemical composition data is obtained from a first data source, for each of the plurality of drilling sites, obtaining well yield data for the drilling site, wherein the well yield data is obtained from a second data source distinct from the first data source, matching the chemical composition data and the well yield data by drilling site, and displaying, on the computer display, a combination of chemical composition and well yield for the plurality of drilling sites. The matching might be based on drill site parameters, such as geographic location and/or geologic characteristics.

Abstract: A computer system and method for processing a mortgage loan receives borrower, property, loan and underwriting data entered by a lender via a certifying company Internet web site. The system, which is operated by the certifying company, communicates with outside service provider systems to order and obtain products there from. The system is loaded with lender parameters for assessing loan risk. The system uses the entered data, outside service provider products and lender parameters to provide a loan assessment and a mortgage loan fulfillment product if the assessment is favorable. The mortgage loan fulfillment product includes a loan package, deed of trust, a promissory note, a title insurance policy and a loan fulfillment certification of the process compliance and data integrity.

Abstract: A computer system and method for processing a mortgage loan receives borrower, property, loan and underwriting data entered by a lender via a certifying company Internet web site. The system, which is operated by the certifying company, communicates with outside service provider systems to order and obtain products there from. The system is loaded with lender parameters for assessing loan risk. The system uses the entered data, outside service provider products and lender parameters to provide a loan assessment and a mortgage loan fulfillment product if the assessment is favorable. The mortgage loan fulfillment product includes a loan package, deed of trust, a promissory note, a title insurance policy and a loan fulfillment certification of the process compliance and data integrity.

Abstract: The invention provides methods and compositions relating to discrete elements of human telomerase and human telomerase RNA. In one embodiment, the invention provides a polynucleotide comprising only one element of human telomerase RNA, wherein the element consists of SEQ ID NO:1, residues 241-330. Such human telomerase RNA elements may be employed in mixtures with a human telomerase polypeptide such as TERT or dyskerin, wherein the polypeptide and polynucleotide specifically interact, and such mixtures may be employed in methods for identifying modulators of a human telomerase polypeptide—human telomerase RNA interaction.

Abstract: The invention provides methods and compositions relating to a human telomerase and related nucleic acids, including four distinct human telomerase subunit proteins called p140, p105, p48 and p43 having human telomerase-specific activity. The proteins may be produced recombinantly from transformed host cells from the disclosed telomerase encoding nucleic acids or purified from human cells. Also included are human telomerase RNA components, as well as specific, functional derivatives thereof. The invention provides isolated telomerase hybridization probes and primers capable of specifically hybridizing with the disclosed telomerase gene, telomerase-specific binding agents such as specific antibodies, and methods of making and using the subject compositions in diagnosis, therapy and in the biopharmaceutical industry.

Abstract: The invention provides methods and compositions rel a human telomerase and related nucleic acids, including four distinct human telomerase subunit proteins called p140, p105, p48 and p43 having human telomerase-specific activity. The proteins may be produced recombinantly from transformed host cells from the disclosed telomerase encoding nucleic acids or purified from human cells. Also included are human telomerase RNA components, as well as specific, functional derivatives thereof. The invention provides isolated telomerase hybridization probes and primers capable of specifically hybridizing with the disclosed telomerase gene, telomerase-specific binding agents such as specific antibodies, and methods of making and using the subject compositions in diagnosis, therapy and in the biopharmaceutical industry.