Patents by Inventor Kay KLAUSING
Kay KLAUSING has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240141427Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low error rate, low phasing rate, or increased incorporation rate for a second generation ffN under reduced incorporation times, as well as methods and kits using the same.Type: ApplicationFiled: September 27, 2023Publication date: May 2, 2024Applicant: ILLUMINA, INC.Inventors: Misha Golynskiy, Rahman Rahman Pour, Jiawen Li, Ryan Craig, Hamed Tabatabaei Ghomi, Saurabh Nirantar, Hsu Myat Noe, Lin Hui Chang, Yvonne Devadas, Jing Wen Lim, Kay Klausing, Humberto Rojo, Eric Murtfeldt, Chris Garcia
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Publication number: 20240141428Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.Type: ApplicationFiled: October 18, 2023Publication date: May 2, 2024Inventors: Robert C. Kain, Xiaohai Liu, Wenyi Feng, Bernard Hirschbein, Helmy A. Eltoukhy, Xiaolin Wu, Geoffrey Paul Smith, Jonathan Mark Boutell, Thomas Joseph, Randall Smith, Min-Jui Richard Shen, Carolyn Tregidgo, Kay Klausing
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Publication number: 20240102067Abstract: This disclosure relates to novel mjresynthesis kits and methods, in particular for use in pairwise sequencing.Type: ApplicationFiled: September 25, 2023Publication date: March 28, 2024Applicant: Illumina, Inc.Inventors: Kay Klausing, Jonathan Boutell, Trina Osothprarop, Oliver Miller, Justin Robbins
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Patent number: 11827932Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.Type: GrantFiled: January 20, 2021Date of Patent: November 28, 2023Assignee: Illumina, Inc.Inventors: Robert C. Kain, Xiaohai Liu, Wenyi Feng, Bernard Hirschbein, Helmy A. Eltoukhy, Xiaolin Wu, Geoffrey Paul Smith, Jonathan Mark Boutell, Thomas Joseph, Randall Smith, Min-Jui Richard Shen, Carolyn Tregidgo, Kay Klausing
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Publication number: 20230357731Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: ApplicationFiled: March 13, 2023Publication date: November 9, 2023Applicants: ILLUMINA, INC., ILLUMINA CAMBRIDGE LIMITED, ILLUMINA SINGAPORE PTE. LTD.Inventors: Kay Klausing, Hamed Tabatabaei Ghomi, Misha Golynskiy, Saurabh Nirantar, Seth McDonald, Sergio Peisajovich
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Publication number: 20230272457Abstract: Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.Type: ApplicationFiled: March 6, 2023Publication date: August 31, 2023Inventors: Boyan Boyanov, Liangliang Qiang, Kevin L. Gunderson, Kay Klausing, Lea Pickering, Cyril Delattre, Tarun Khurana
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Publication number: 20230212667Abstract: Polynucleotide sequencing methods for sequencing one or more polynucleotide templates use primers bound to a surface as sequencing primers. The surface primers may include at least a portion of a surface oligonucleotide used during cluster formation. The sequencing methods may be used for single stranded sequencing or double stranded sequencing. Double stranded sequencing methods may employ an enzyme that has nick-translation activity. A kit includes all the reagents needed for sequencing does not include sequencing primers. The kit may be used to accomplish the sequencing methods of the present disclosure.Type: ApplicationFiled: December 28, 2022Publication date: July 6, 2023Applicants: ILLUMINA CAMBRIDGE LIMITED, ILLUMINA, INC.Inventors: Jonathan Mark Boutell, Eli M. Carrami, Pietro Gatti Lafranconi, Philip Balding, Oliver Jon Miller, Kay Klausing, Justin Robbins, Xiaolin Wu
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Patent number: 11634697Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: GrantFiled: April 9, 2021Date of Patent: April 25, 2023Assignees: Illumina, Inc., Illumina Cambridge Limited, Illumina Singapore Pte. Ltd.Inventors: Kay Klausing, Hamed Tabatabaei Ghomi, Misha Golynskiy, Saurabh Nirantar, Seth McDonald, Sergio Peisajovich
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Patent number: 11629373Abstract: Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.Type: GrantFiled: March 27, 2020Date of Patent: April 18, 2023Assignee: Illumina, Inc.Inventors: Boyan Boyanov, Liangliang Qiang, Kevin L. Gunderson, Kay Klausing, Lea Pickering, Cyril Delattre, Tarun Khurana
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Publication number: 20230047225Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low pre-phasing rates when using ambiently stored polymerases, as well as methods and kits using the same.Type: ApplicationFiled: June 1, 2022Publication date: February 16, 2023Applicants: ILLUMINA, INC., ILLUMINA CAMBRIDGE LIMITED, ILLUMINA SINGAPORE PTE. LTD.Inventors: Misha Golynskiy, Mariko Matsuura, Saurabh Nirantar, Hamed Tabatabaei Ghomi, Seth McDonald, Ryan Craig, Sergio Peisajovich, Kyrie Johnson, Kay Klausing, Eric Murtfeldt, Alexandra Exner
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Publication number: 20220371005Abstract: A system includes a fluidic device, a flow control valve, a first reagent fluid reservoir fluidly connectable to the fluidic device by the flow control valve, a first fluid buffer reservoir fluidly connectable to the fluidic device by the flow control valve, and a common fluid buffer source fluidly connectable to the fluidic device by the flow control valve. The flow control valve permits flow comprising: (i) flow from the first reagent fluid reservoir to the fluidic device, (ii) flow from the common fluid buffer source to the fluidic device, (iii) flow from the fluidic device to the first fluid buffer reservoir, (iv) flow from the first reagent fluid reservoir to the fluidic device, and (v) flow from the first fluid buffer reservoir to the fluidic device.Type: ApplicationFiled: August 3, 2022Publication date: November 24, 2022Applicant: Illumina, Inc.Inventors: Wesley A. COX-MURANAMI, Kay KLAUSING, Bradley Kent DREWS, Nicholas WATSON, Jennifer Olivia FOLEY, Murphy HITCHCOCK, Paul SANGIORGIO, Sz-Chin Steven LIN
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Patent number: 11426723Abstract: A system includes a fluidic device, a flow control valve, a first reagent fluid reservoir fluidly connectable to the fluidic device by the flow control valve, a first fluid buffer reservoir fluidly connectable to the fluidic device by the flow control valve, and a common fluid buffer source fluidly connectable to the fluidic device by the flow control valve. The flow control valve permits flow comprising: (i) flow from the first reagent fluid reservoir to the fluidic device, (ii) flow from the common fluid buffer source to the fluidic device, (iii) flow from the fluidic device to the first fluid buffer reservoir, (iv) flow from the first reagent fluid reservoir to the fluidic device, and (v) flow from the first fluid buffer reservoir to the fluidic device.Type: GrantFiled: September 26, 2019Date of Patent: August 30, 2022Assignee: Illumina, Inc.Inventors: Wesley A. Cox-Muranami, Kay Klausing, Bradley Kent Drews, Nicholas Watson, Jennifer Olivia Foley, Murphy Hitchcock, Paul Sangiorgio, Sz-Chin Steven Lin
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Publication number: 20210348141Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: ApplicationFiled: April 9, 2021Publication date: November 11, 2021Applicants: Illumina, Inc., Illumina Cambridge Limited, Illumina Singapore Pte. Ltd.Inventors: Kay Klausing, Hamed Tabatabaei Ghomi, Misha Golynskiy, Saurabh Nirantar, Seth McDonald, Sergio Peisajovich
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Publication number: 20210139978Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.Type: ApplicationFiled: January 20, 2021Publication date: May 13, 2021Inventors: Robert C. Kain, Xiaohai Liu, Wenyi Feng, Bernard Hirschbein, Helmy A. Eltoukhy, Xiaolin Wu, Geoffrey Paul Smith, Jonathan Mark Boutell, Thomas Joseph, Randall Smith, Min-Jui Richard Shen, Carolyn Tregidgo, Kay Klausing
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Patent number: 11001816Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: GrantFiled: December 4, 2019Date of Patent: May 11, 2021Assignees: Illumina, Inc., Illumina Singapore PTE. LTD., Illumina Cambridge LimitedInventors: Kay Klausing, Hamed Tabatabaei Ghomi, Misha Golynskiy, Saurabh Nirantar, Seth McDonald, Sergio Peisajovich
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Patent number: 10900077Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.Type: GrantFiled: May 7, 2019Date of Patent: January 26, 2021Assignee: ILLUMINA, INC.Inventors: Robert C. Kain, Xiaohai Liu, Wenyi Feng, Bernard Hirschbein, Helmy A. Eltoukhy, Xiaolin Wu, Geoffrey Paul Smith, Jonathan Mark Boutell, Thomas Joseph, Randall Smith, Min-Jui Richard Shen, Carolyn Tregidgo, Kay Klausing
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Publication number: 20200318167Abstract: Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.Type: ApplicationFiled: March 27, 2020Publication date: October 8, 2020Inventors: Boyan Boyanov, Liangliang Qiang, Kevin L. Gunderson, Kay Klausing, Lea Pickering, Cyril Delattre, Tarun Khurana
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Publication number: 20200181587Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: ApplicationFiled: December 4, 2019Publication date: June 11, 2020Applicants: Illumina, Inc., Illumina Cambridge Limited, Illumina Singapore Pte. Ltd.Inventors: Kay Klausing, Hamed Tabatabael Ghomi, Misha Golynskiy, Saurabh Nirantar, Seth McDonald, Sergio Peisajovich
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Patent number: 10633694Abstract: Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.Type: GrantFiled: September 10, 2015Date of Patent: April 28, 2020Assignee: Illumina, Inc.Inventors: Boyan Boyanov, Liangliang Qiang, Kevin L. Gunderson, Kay Klausing, Lea Pickering, Cyril Delattre, Tarun Khurana
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Publication number: 20200108382Abstract: A system includes a fluidic device, a flow control valve, a first reagent fluid reservoir fluidly connectable to the fluidic device by the flow control valve, a first fluid buffer reservoir fluidly connectable to the fluidic device by the flow control valve, and a common fluid buffer source fluidly connectable to the fluidic device by the flow control valve. The flow control valve permits flow comprising: (i) flow from the first reagent fluid reservoir to the fluidic device, (ii) flow from the common fluid buffer source to the fluidic device, (iii) flow from the fluidic device to the first fluid buffer reservoir, (iv) flow from the first reagent fluid reservoir to the fluidic device, and (v) flow from the first fluid buffer reservoir to the fluidic device.Type: ApplicationFiled: September 26, 2019Publication date: April 9, 2020Applicant: Illumina, Inc.Inventors: Wesley A. Cox-Muranami, Kay Klausing, Bradley Kent Drews, Nicholas Watson, Jennifer Olivia Foley, Murphy Hitchcock, Paul Sangiorgio, Sz-Chin Steven Lin