Abstract: A biological observation apparatus is configured as follows. Namely, the biological observation apparatus includes a marker attached to a living body in order to detect the vibration of the living body, a high-sensitivity camera which forms an observation image of the living body, a high-speed camera which forms an image of light from the marker, and an optical system including a first BA which prevents the light from the marker from entering the high-sensitivity camera.

Abstract: Dynamic movement of a specimen in a pressing direction and in directions intersecting the pressing direction is effectively suppressed, while maintaining the viability of the specimen. A stabilizer for in vivo examination that is placed in contact with a biological specimen during examination of the specimen to suppress movement thereof includes a contact-area increasing portion that is provided on a contact surface with the biological specimen and that is configured to increase the contact area with the biological specimen by virtue of a pressure applied during the contact.

Abstract: A biological observation apparatus is configured as follows. Namely, the biological observation apparatus includes a marker attached to a living body in order to detect the vibration of the living body, a high-sensitivity camera which forms an observation image of the living body, a high-speed camera which forms an image of light from the marker, and an optical system including a first BA which prevents the light from the marker from entering the high-sensitivity camera.

Abstract: A cell culture detection apparatus includes a culture vessel that houses cells together with a culture liquid, a culturing device that cultures the cells under predetermined culturing conditions, a detection device that detects a feature of the cells among the cells being cultured, and a light blocking device that blocks the culture vessel from environmental light when the feature is not detected.

Abstract: Dynamic movement of a specimen in a pressing direction and in directions intersecting the pressing direction is effectively suppressed, while maintaining the viability of the specimen. A stabilizer for in vivo examination that is placed in contact with a biological specimen during examination of the specimen to suppress movement thereof includes a contact-area increasing portion that is provided on a contact surface with the biological specimen and that is configured to increase the contact area with the biological specimen by virtue of a pressure applied during the contact.

Abstract: A microscope imaging apparatus and a biological-specimen examination system that can accurately carry out measurement even for an object under examination having substantial brightness non-uniformity are provided. The microscope imaging apparatus includes a stage that holds the object under examination, an illumination unit that illuminates the object under examination, an image-acquisition unit that acquires images of the object under examination, and a motion unit that moves the stage and the image-acquisition unit relative to each other. The image-acquisition unit includes an imaging device capable of image acquisition using a time delay integration method. When acquiring a plurality of images of the object under examination, the exposure time during which accumulated charge is produced in the imaging device is made different for each of the acquired images, and the plurality of images are combined into a single image.

Abstract: The present invention provides a living-specimen observation and measurement system for storing a predetermined observation condition and controlling the predetermined observation condition based on an activity specific to a living specimen. The predetermined observation condition is information (items) about, for example, observation and culturing. More specifically, the predetermined observation condition includes the type of a living specimen, the incubation temperature, the pH, the seeding concentration, the cell density, time elapsed from seeding, the fluorochrome type, the fluorochrome concentration, the presence of delivered drug, the amount of light radiated onto the living specimen (e.g., irradiation light intensity per cell, irradiation light illuminance, etc.), the wavelength of irradiation light, the continuity of irradiation light (CW, pulse frequency, pulse width), the duration of a single irradiation, the number of observations, the observation interval, and so forth.

Abstract: A microscope imaging apparatus and a biological-specimen examination system that can accurately carry out measurement even for an object under examination having substantial brightness non-uniformity are provided. The microscope imaging apparatus includes a stage that holds the object under examination, an illumination unit that illuminates the object under examination, an image-acquisition unit that acquires images of the object under examination, and a motion unit that moves the stage and the image-acquisition unit relative to each other. The image-acquisition unit includes an imaging device capable of image acquisition using a time delay integration method. When acquiring a plurality of images of the object under examination, the exposure time during which accumulated charge is produced in the imaging device is made different for each of the acquired images, and the plurality of images are combined into a single image.

Abstract: A microscope imaging apparatus and a biological-specimen examination system that can accurately carry out measurement even for an object under examination having substantial brightness non-uniformity are provided. The microscope imaging apparatus includes a stage that holds the object under examination, an illumination unit that illuminates the object under examination, an image-acquisition unit that acquires images of the object under examination, and a motion unit that moves the stage and the image-acquisition unit relative to each other. The image-acquisition unit includes an imaging device capable of image acquisition using a time delay integration method. When acquiring a plurality of images of the object under examination, the exposure time during which accumulated charge is produced in the imaging device is made different for each of the acquired images, and the plurality of images are combined into a single image.

Abstract: A microscope imaging apparatus and a biological-specimen examination system that can accurately carry out measurement even for an object under examination having substantial brightness non-uniformity are provided. The microscope imaging apparatus includes a stage that holds the object under examination, an illumination unit that illuminates the object under examination, an image-acquisition unit that acquires images of the object under examination, and a motion unit that moves the stage and the image-acquisition unit relative to each other. The image-acquisition unit includes an imaging device capable of image acquisition using a time delay integration method. When acquiring a plurality of images of the object under examination, the exposure time during which accumulated charge is produced in the imaging device is made different for each of the acquired images, and the plurality of images are combined into a single image.

Abstract: The present invention provides a biological sample culturing and observation system which includes: an incubator in which a biological samples are housed, and whose interior is maintained in a culturing environment satisfying predetermined conditions, and the interior is isolated from the outside; an observation optical system that from outside the incubator optically observes the biological sample via the incubator; a light blocking device that blocks external light that is irradiated on the biological sample and within the visual field of a observation optical system; and a supply device that selectively supplies a liquid or gas to the biological sample inside the incubator from a plurality of holding vessels that individually hold a plurality of different types of liquid or gas that are necessary for the culturing.

Abstract: A biological sample observation system wherein the fluorescence intensity of living cells and the like can be measured accurately in real time, and damage to the living cells can be reduced, and a biological sample observation method using the system are provided. A biological sample observation system for observing change with time in a biological sample being cultured, comprises: a culturing space where an inside environment is maintained at a predetermined level, and the biological sample is cultured under the environment; a buffering space which is formed outside of the culturing space to relieve the effect on the culturing space from the outside of the space, and which is substantially separated from the outside; and a detector which observes the biological sample inside the culturing space through at least a part of the buffering space.

Abstract: A microscope imaging apparatus and a biological-specimen examination system that can accurately carry out measurement even for an object under examination having substantial brightness non-uniformity are provided. The microscope imaging apparatus includes a stage that holds the object under examination, an illumination unit that illuminates the object under examination, an image-acquisition unit that acquires images of the object under examination, and a motion unit that moves the stage and the image-acquisition unit relative to each other. The image-acquisition unit includes an imaging device capable of image acquisition using a time delay integration method. When acquiring a plurality of images of the object under examination, the exposure time during which accumulated charge is produced in the imaging device is made different for each of the acquired images, and the plurality of images are combined into a single image.

Abstract: An object is to provide a biological sample observation system wherein light damage to the biological sample is decreased, and observation can be performed accurately and quickly over a long period of time.

Abstract: The present invention improves the observation efficiency and makes possible detecting predetermined data from cells in a culture by a small scale and simple scanning optical system. The invention provides a cell cultivating and detecting unit comprising a stationary light source that generates a collimated light, an irradiation unit that irradiates the cells with the collimated light incident from the light source and emits the light emitted from the cells; a detecting unit that detects the light emitted from the irradiation unit, and wherein the irradiation unit can move in a direction parallel to the collimated light.

Abstract: A cell culture detection apparatus includes a culture vessel that houses cells together with a culture liquid, a culturing device that cultures the cells under predetermined culturing conditions, a detection device that detects a feature of the cells among the cells being cultured, and a light blocking device that blocks the culture vessel from environmental light when the feature is not detected.