Abstract: A chemical agent-spraying device is provided with a chemical agent tank and chemical agent-jetting parts that jet out a chemical agent supplied from the chemical agent tank. The chemical agent-jetting parts include a first chemical agent-jetting part that jets out the chemical agent onto the reverse surfaces of crop leaves as an area to which the chemical agent is to be jetted, and a second chemical agent-jetting part that jets out the chemical agent onto the obverse surfaces of crop leaves as an area to which the chemical agent is to be jetted.

Abstract: A spraying machine includes a machine body, a support frame, and a spraying portion. The support frame includes a horizontal frame having a length along a left-right direction, and a pair of vertical frames and each having a length along an up-down direction, and protruding downward from both ends of the horizontal frame. The spraying portion is supported by the support frame, and sprays a spraying material. The support frame is supported by the machine body to be rotatable around a rotation axis passing through a fulcrum portion provided on the horizontal frame and extending along a front-back direction, while maintaining a relative positional relationship between the pair of vertical frames and the horizontal frame.

Abstract: The present invention provides a method for high yield and stable production of carotenoids through microbial culture. The present invention provides a method for culture of carotenoid-producing bacteria, which comprises culturing carotenoid-producing bacteria in a medium containing cobalt or a cobalt salt at a concentration of 0.005 ?mol/L to 20 ?mol/L.

Abstract: The present invention provides a method for high yield and stable production of carotenoids through microbial culture. The present invention provides a method for culture of carotenoid-producing bacteria, which comprises culturing carotenoid-producing bacteria in a medium containing cobalt or a cobalt salt at a concentration of 0.005 ?mol/L to 20 ?mol/L.

Abstract: An object of the present invention is to provide a method for microbioiogically producing astaxanthin of high concentration at low cost while suppressing production of canthaxanthin. Specifically, the present invention relates to a method for producing carotenoids including astaxanthin comprising culturing a bacterium that concurrently produces astaxanthin and canthaxanthin in a medium containing biotin, wherein a ratio of concentration of produced canthaxanthin to concentration of produced astaxanthin in a culture product after the end of culture in the medium is lower than that in a culture product alter the end of culture in a biotin-free medium.

Abstract: Methods of obtaining a carotenoid-containing composition were developed, in which degradation of carotenoids is suppressed even though extraction and purification are performed on a culture of microorganism that produces carotenoids including astaxanthin by simple steps using a lower alcohol which is a conventionally known solvent, and the compositions have high astaxanthin content and contain highly pure carotenoids and also have satisfactory crystal properties. Further addition of a simple step that promotes isomerization of astaxanthin successfully improved the carotenoid yield.

Abstract: An object of the present invention is to provide a method for microbiologically producing astaxanthin of high concentration at low cost while suppressing production of canthaxanthin. Specifically, the present invention relates to a method for producing carotenoids including astaxanthin comprising culturing a bacterium that concurrently produces astaxanthin and canthaxanthin in a medium containing biotin, wherein a ratio of concentration of produced canthaxanthin to concentration of produced astaxanthin in a culture product after the end of culture in the medium is lower than that in a culture product after the end of culture in a biotin-free medium.

Abstract: An object of the present invention is to provide a method for microbioiogically producing astaxanthin of high concentration at low cost while suppressing production of canthaxanthin. Specifically, the present invention relates to a method for producing carotenoids including astaxanthin comprising culturing a bacterium that concurrently produces astaxanthin and canthaxanthin in a medium containing biotin, wherein a ratio of concentration of produced canthaxanthin to concentration of produced astaxanthin in a culture product after the end of culture in the medium is lower than that in a culture product alter the end of culture in a biotin-free medium.

Abstract: Methods of obtaining a carotenoid-containing composition were developed, in which degradation of carotenoids is suppressed even though extraction and purification are performed on a culture of microorganism that produces carotenoids including astaxanthin by simple steps using a lower alcohol which is a conventionally known solvent, and the compositions have high astaxanthin content and contain highly pure carotenoids and also have satisfactory crystal properties. Further addition of a simple step that promotes isomerization of astaxanthin successfully improved the carotenoid yield.

Abstract: The present invention provides a salmon flesh color improving method and a salmon flesh color improving feed which are usable for producing a salmon having a mild reddish orange flesh color close to the flesh color of wild fish, and a salmon and fish flesh thereof produced by such a flesh color improving method. The present invention also provides a method for producing a salmon having a mild reddish orange flesh color, comprising cultivating the salmon with a feed comprising a carotenoid colorant mixed therein, the carotenoid colorant comprising at least astaxanthin, phoenicoxanthin, canthaxanthin and adonixanthin.

Abstract: The present invention provides a method for producing a carotenoid, which comprises culturing a carotenoid-producing bacterium in an amino acid-supplemented medium, and collecting the carotenoid from the resulting cultured product, wherein the amino acid is at least one selected, from the group consisting of glutamic acid, aspartic acid, glutamine, asparagine, alanine, glycine, serine, threonine, arginine, tyrosine, proline, phenylalanine and leucine, and salts thereof.

Abstract: A method for microbiologically producing zeaxanthin at high concentration and low cost while suppressing production of gluconic acid is presented. Specifically, a method for producing carotenoids containing zeaxanthin by culturing a bacterium producing carotenoids containing zeaxanthin in a medium containing biotin is provided.

Abstract: The present invention provides a method for collecting a carotenoid from a culture of a carotenoid-producing bacterium at high yield. Specifically, the present invention provides a method for separating a carotenoid comprising a step of precipitating a concentrate containing the carotenoid from a culture of a carotenoid-producing bacterium under acidic conditions; and a method for producing a carotenoid comprising the steps of precipitating a concentrate containing the carotenoid from a culture of a carotenoid-producing bacterium under acidic conditions and collecting the carotenoid from the obtained precipitate.

Abstract: A method for microbiologically producing zeaxanthin at high concentration and low cost while suppressing production of gluconic acid is presented. Specifically, a method for producing carotenoids containing zeaxanthin by culturing a bacterium producing carotenoids containing zeaxanthin in a medium containing biotin is provided.

Abstract: An object of the present invention is to provide a method for microbiologically producing astaxanthin of high concentration at low cost while suppressing production of canthaxanthin. Specifically, the present invention relates to a method for producing carotenoids including astaxanthin comprising culturing a bacterium that concurrently produces astaxanthin and canthaxanthin in a medium containing biotin, wherein a ratio of concentration of produced canthaxanthin to concentration of produced astaxanthin in a culture product after the end of culture in the medium is lower than that in a culture product after the end of culture in a biotin-free medium.

Abstract: The present invention provides a method for collecting a carotenoid from a culture of a carotenoid-producing bacterium at high yield. Specifically, the present invention provides a method for separating a carotenoid comprising a step of precipitating a concentrate containing the carotenoid from a culture of a carotenoid-producing bacterium under acidic conditions; and a method for producing a carotenoid comprising the steps of precipitating a concentrate containing the carotenoid from a culture of a carotenoid-producing bacterium under acidic conditions and collecting the carotenoid from the obtained precipitate.

Abstract: A method for producing a composition containing carotenoid at a content of 80% or greater, characterized in performing extraction on a culture of a microorganism, a concentrate of the culture, or a dried substance thereof with at least one selected from the group consisting of lower alcohols, water-containing lower alcohols, lower dialkylketones and ethers; and then washing a precipitate, obtained by concentrating the extract solution, with at least one selected from the group consisting of lower alcohols, water-containing lower alcohols, hydrocarbon-based solvents and lower dialkylketones; and food, a pharmaceutical composition or a cosmetic substance comprising the carotenoid-containing composition.

Abstract: The present invention provides a method for producing a carotenoid, which comprises culturing a carotenoid-producing bacterium in an amino acid-supplemented medium, and collecting the carotenoid from the resulting cultured product, wherein the amino acid is at least one selected, from the group consisting of glutamic acid, aspartic acid, glutamine, asparagine, alanine, glycine, serine, threonine, arginine, tyrosine, proline, phenylalanine and leucine, and salts thereof

Abstract: The present invention provides a salmon flesh color improving method and a salmon flesh color improving feed which are usable for producing a salmon having a mild reddish orange flesh color close to the flesh color of wild fish, and a salmon and fish flesh thereof produced by such a flesh color improving method. The present invention also provides a method for producing a salmon having a mild reddish orange flesh color, comprising cultivating the salmon with a feed comprising a carotenoid colorant mixed therein, the carotenoid colorant comprising at least astaxanthin, phoenicoxanthin, canthaxanthin and adonixanthin.

Abstract: The present invention relates to a process for producing zeaxanthin, ?-carotene, or lycopene, comprising inducing mutation in a carotenoid-producing microorganism in which the base sequence of DNA corresponding to 16S ribosomal RNA is substantially homologous to the base sequence described in SEQ ID NO: 1; screening for a mutant strain having a high product proportion of zeaxanthin, ?-carotene, or lycopene to the whole production amount of carotenoids to provide a microorganism producing zeaxanthin, ?-carotene, or lycopene; culturing the mutant microorganism; and harvesting zeaxanthin, ?-carotene, lycopene or a carotenoid mixture containing the same from the resultant culture.