Patents by Inventor Kazuhito Miyauchi
Kazuhito Miyauchi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9360432Abstract: The present invention provides a method for a simple and accurate measurement of triglycerides in low-density lipoprotein in a sample comprising performing the following steps sequentially: (i) a step of generating free glycerol by allowing lipoprotein lipase to act on a sample, in an aqueous medium comprising the sample and a specific surfactant such as polyoxyethylene polyoxyalkylene polycyclic phenyl ether; (ii) a step of removing free glycerol present in the reaction solution of the above step (i); (iii) a step of generating free glycerol by allowing lipoprotein lipase to act on the reaction solution from which free glycerol has been removed in step (ii), in the presence of a specific surfactant such as polyoxyethylene polyoxyalkylene alkyl ether; and (iv) a step of measuring free glycerol generated in step (iii), and a kit used for the method.Type: GrantFiled: October 31, 2006Date of Patent: June 7, 2016Assignee: KYOWA MEDEX CO., LTD.Inventors: Yuki Katayama, Kazuhito Miyauchi, Shizuyo Takada, Tomomi Murakami
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Patent number: 9051600Abstract: Provided is a method for simply and accurately measuring sphingomyelin in a sample, and a kit therefore. The method is a method for measuring sphingomyelin in a sample, comprising reacting the sample with a phospholipase D which does not react with sphingomyelin and lysophosphatidylcholine but reacts with phosphatidylcholine, a lysophospholipase or a monoglyceride lipase, and a choline oxidase, eliminating the formed hydrogen peroxide, reacting the resultant with a phospholipase D which does not react with glycerol-3-phosphorylcholine and free fatty acid but reacts with sphingomyelin, and a choline oxidase, and measuring the formed hydrogen peroxide.Type: GrantFiled: July 25, 2012Date of Patent: June 9, 2015Assignee: KYOWA MEDEX CO., LTD.Inventors: Takehide Kimura, Kazuhito Miyauchi, Hideyuki Kuwata
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Publication number: 20140162300Abstract: Provided is a method for simply and accurately measuring sphingomyelin in a sample, and a kit therefor. The method is a method for measuring sphingomyelin in a sample, comprising reacting the sample with a phospholipase D which does not react with sphingomyelin and lysophosphatidylcholine but reacts with phosphatidylcholine, a lysophospholipase or a monoglyceride lipase, and a choline oxidase, eliminating the formed hydrogen peroxide, reacting the resultant with a phospholipase D which does not react with glycerol-3-phosphorylcholine and free fatty acid but reacts with sphingomyelin, and a choline oxidase, and measuring the formed hydrogen peroxide.Type: ApplicationFiled: July 25, 2012Publication date: June 12, 2014Applicant: KYOWA MEDEX CO., LTD.Inventors: Takehide Kimura, Kazuhito Miyauchi, Hideyuki Kuwata
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Patent number: 8119360Abstract: The present invention provides a method, a reagent and a kit for simple and sensitive determination of cholesterol in remnant-like particles without separation of components of a sample. A method for quantitatively determining remnant-like particle cholesterol in a sample, which comprises: in an aqueous medium containing the sample and in the presence of a combination of specific surfactants and a phospholipid-hydrolyzing enzyme, allowing remnant-like particle cholesterol in the sample to react with cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase (in the presence of oxidized coenzyme); and determining the formed hydrogen peroxide or reduced coenzyme.Type: GrantFiled: February 14, 2006Date of Patent: February 21, 2012Assignee: Kyowa Medex Co., Ltd.Inventors: Kazuhito Miyauchi, Mayumi Fujinaka
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Publication number: 20090226944Abstract: The present invention provides a method for a simple and accurate measurement of triglycerides in low-density lipoprotein in a sample comprising performing the following steps sequentially: (i) a step of generating free glycerol by allowing lipoprotein lipase to act on a sample, in an aqueous medium comprising the sample and a specific surfactant such as polyoxyethylene polyoxyalkylene polycyclic phenyl ether; (ii) a step of removing free glycerol present in the reaction solution of the above step (i); (iii) a step of generating free glycerol by allowing lipoprotein lipase to act on the reaction solution from which free glycerol has been removed in step (ii), in the presence of a specific surfactant such as polyoxyethylene polyoxyalkylene alkyl ether; and (iv) a step of measuring free glycerol generated in step (iii), and a kit used for the method.Type: ApplicationFiled: October 31, 2006Publication date: September 10, 2009Applicant: KYOWA MEDEX CO., LTD.Inventors: Yuki Katayama, Kazuhito Miyauchi, Shizuyo Takada, Tomomi Murakami
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Publication number: 20090170139Abstract: The present invention provides a method for simple and accurate measurement of cholesterol in remnant-like lipoprotein (RLP) (RLP-C) in a sample, which comprises the steps of: (i) eliminating cholesterol in lipoproteins other than RLP by reacting the sample with cholesterol oxidase, or cholesterol dehydrogenase and oxidized coenzyme, in an aqueous medium comprising surfactant A; (ii) reacting the reaction solution from which cholesterol in lipoproteins other than RLP has been eliminated in the above step (i) with cholesterol esterase and cholesterol oxidase, or cholesterol esterase, cholesterol dehydrogenase and oxidized coenzyme, in the presence of surfactant B to form hydrogen peroxide or reduced coenzyme; and (iii) measuring the hydrogen peroxide or reduced coenzyme formed in the above step (ii).Type: ApplicationFiled: December 8, 2006Publication date: July 2, 2009Applicant: Kyowa Medex Co., Ltd.Inventors: Shingo Mishima, Kazuhito Miyauchi
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Publication number: 20090023167Abstract: The present invention provides a method, a reagent and a kit for simple and sensitive determination of cholesterol in remnant-like particles without separation of components of a sample. A method for quantitatively determining remnant-like particle cholesterol in a sample, which comprises: in an aqueous medium containing the sample and in the presence of a combination of specific surfactants and a phospholipid-hydrolyzing enzyme, allowing remnant-like particle cholesterol in the sample to react with cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase (in the presence of oxidized coenzyme); and determining the formed hydrogen peroxide or reduced coenzyme.Type: ApplicationFiled: February 14, 2006Publication date: January 22, 2009Applicant: KYOWA MEDEX CO., LTD.Inventors: Kazuhito Miyauchi, Mayumi Fujinaka
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Publication number: 20070161068Abstract: According to the present invention, esterified and free cholesterol in very low-density lipoprotein remnant (hereinafter collectively referred to as very low-density lipoprotein remnant cholesterol) in a sample is determined in an aqueous medium containing the sample in the presence of a combination of (a) a polyoxyethylene-polyoxyalkylene alkylaryl ether and (b) one kind of surfactant selected from the group consisting of a polyoxyethylene-polyoxybutylene copolymer, a polyoxyethylene styrenated-phenyl ether and a polyoxyalkylene long-chain alkyl ether which combination is capable of causing cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase to act specifically on very low-density lipoprotein remnant cholesterol.Type: ApplicationFiled: January 28, 2005Publication date: July 12, 2007Applicant: Kyowa Medex Co., LTD.Inventor: Kazuhito Miyauchi
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Patent number: 7202047Abstract: The present invention provides a simple and sensitive aqueous method for determining cholesterol in remnant-like particles in a biological sample without the separation of components of the sample. The method comprises contacting the sample with (i) cholesterol esterase, (ii) cholesterol oxidase or cholesterol dehydrogenase, and (iii) phospholipase with a surfactant that inhibits cholesterol oxidase or cholesterol dehydrogenase in lipoproteins other than remnant-like particles in the presence of oxygen or an oxidized coenzyme. Formed hydrogen peroxide or reduced coenzyme is measured and correlated to a quantitative determination of cholesterol in remnant-like particles.Type: GrantFiled: May 6, 2003Date of Patent: April 10, 2007Assignee: Kyowa Medex Co., Ltd.Inventor: Kazuhito Miyauchi
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Publication number: 20050130323Abstract: The present invention provides an inhibitor for adhesion of a metal hydroxide-containing substance to a waste liquid line of an autoanalyzer comprising a polysaccharide; a method of inhibiting adhesion of a metal hydroxide-containing substance to a waste liquid line of an autoanalyzer wherein the inhibitor is used; a reagent for measuring a biological component in a sample, which is effective for inhibiting adhesion of a metal hydroxide-containing substance to a waste liquid line, said reagent containing the said inhibitor; and a method for measuring a biological component in a sample wherein the reagent for measuring a biological component is used.Type: ApplicationFiled: February 17, 2003Publication date: June 16, 2005Inventor: Kazuhito Miyauchi
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Publication number: 20050042703Abstract: The present invention relates to a method for conveniently quantitating TGs contained in various lipoproteins. A method for quantitating trigyceride (TG) in a particular lipoprotein which comprises eliminating free glycerol from a sample containing free glycerol and TG in the particular lipoprotein, then allowing the resulting sample to react with lipoprotein lipase and an enzyme system which generates hydrogen peroxide from free glycerol, and quantitating the generated hydrogen peroxide, is provided.Type: ApplicationFiled: September 29, 2004Publication date: February 24, 2005Applicant: KYOWA MEDEX CO., LTD.Inventors: Kazuhito Miyauchi, Shizuyo Takada, Tomomi Murakami, Akira Miike
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Patent number: 6811994Abstract: The present invention relates to a method for conveniently quantitating TGs contained in various lipoproteins. A method for quantitating trigyceride (TG) in a particular lipoprotein which comprises eliminating free glycerol from a sample containing free glycerol and TG in the particular lipoprotein, then allowing the resulting sample to react with lipoprotein lipase and an enzyme system which generates hydrogen peroxide from free glycerol, and quantitating the generated hydrogen peroxide, is provided.Type: GrantFiled: July 20, 2001Date of Patent: November 2, 2004Assignee: Kyowa Medex Co., Ltd.Inventors: Kazuhito Miyauchi, Shizuyo Takada, Tomomi Murakami, Akira Miike
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Publication number: 20030207342Abstract: The present invention provides a method and a reagent for the simple and sensitive determination of cholesterol in remnant-like particles in a biological sample without the separation of components of the sample. The method comprises contacting the sample with (i) cholesterol esterase, (ii) cholesterol oxidase or cholesterol dehydrogenase, and (iii) phospholipase in the presence of oxygen or an oxidized coenzyme, and measuring the formed hydrogen peroxide or reduced coenzyme. The reagent comprises (i) cholesterol esterase, (ii) cholesterol oxidase or cholesterol dehydrogenase, and (iii) phospholipase.Type: ApplicationFiled: May 6, 2003Publication date: November 6, 2003Applicant: KYOWA MEDEX CO., LTD.Inventor: Kazuhito Miyauchi
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Publication number: 20010031479Abstract: The present invention provides a method and a reagent for the simple and sensitive determination of cholesterol in remnant-like particles in a biological sample without the separation of components of the sample. The method comprises contacting the sample with (i) cholesterol esterase, (ii) cholesterol oxidase or cholesterol dehydrogenase, and (iii) phospholipase in the presence of oxygen or an oxidized coenzyme, and measuring the formed hydrogen peroxide or reduced coenzyme. The reagent comprises (i) cholesterol esterase, (ii) cholesterol oxidase or cholesterol dehydrogenase, and (iii) phospholipase.Type: ApplicationFiled: February 21, 2001Publication date: October 18, 2001Inventor: Kazuhito Miyauchi
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Patent number: 5888755Abstract: Provided is a method of determining the amount of cholesterol in high-density lipoprotein (HDL), which comprises reacting an HDL-containing sample with cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase in the presence of a reagent for aggregating lipoproteins- except HDL, and determining the amount of hydrogen peroxide or reductive co-enzyme formed therein.Type: GrantFiled: November 10, 1997Date of Patent: March 30, 1999Assignee: Kyowa Medex Co., Ltd.Inventors: Kazuhito Miyauchi, Akira Miike, Eiko Shutoh, Hiroyuki Sugiuchi, Tetsumi Irie, Kaneto Uekama, Susumu Ohsawa
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Patent number: 5858695Abstract: Disclosed are a method for quantitative determination of bilirubin and a reagent for the method, which are useful in clinical examinations. The method comprises subjecting the bilirubin in a sample to coexist with ascorbate oxidase and a reaction promoter in aqueous medium to thereby oxidize the bilirubin, measuring the change in absorbance of the aqueous medium and comparing the change in absorbance with a calibration curve. The reagent comprises ascorbate oxidase and a reaction promoter.Type: GrantFiled: October 22, 1996Date of Patent: January 12, 1999Assignee: Kyowa Medex Co., LTD.Inventors: Akira Kadota, Kayoko Shigenobu, Akira Miike, Kazuhito Miyauchi
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Patent number: 5807696Abstract: The present invention relates to a method for the determination of cholesterol in low-density lipoprotein (LDL) in a sample containing LDL, which comprises eliminating cholesterol in high-density lipoprotein in the sample, subjecting the sample to a reaction utilizing the action of a cholesterol ester-hydrolyzing enzyme and the action of a cholesterol-oxidizing enzyme or of cholesterol oxidoreductase, and determining the amount of hydrogen peroxide or a reduced type coenzyme generated by the reaction.Type: GrantFiled: November 13, 1996Date of Patent: September 15, 1998Assignee: Kyowa Medex Co., Ltd.Inventors: Kazuhito Miyauchi, Akira Miike
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Patent number: 5736406Abstract: Provided are a method of determining the amount of cholesterol in high-density lipoprotein (HDL), which comprises measuring the amount of cholesterol in low-density lipoprotein (LDL), very-low-density lipoprotein (VLDL) and chylomicron (CM) in a sample in the presence of a sugar compound and/or a protein solubilizing agent, and calculating the difference between the amount of cholesterol in LDL, VLDL and CM and the total amount of cholesterol in the sample, and a method of determining the amount of cholesterol in HDL, which comprises measuring the amount of cholesterol in HDL in a sample in the presence of a sugar compound and/or a protein solubilizing agent.Type: GrantFiled: November 2, 1995Date of Patent: April 7, 1998Assignee: Kyowa Medex Co., Ltd.Inventors: Kazuhito Miyauchi, Norihiko Kayahara, Toshio Tatano, Eiko Shutoh, Hiroyuki Sugiuchi, Tetsumi Irie, Kaneto Uekama, Susumu Ohsawa
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Patent number: 5691159Abstract: Provided is a method of determining the amount of cholesterol in high-density lipoprotein (HDL), which comprises reacting an HDL-containing sample with cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase in the presence of a reagent for aggregating lipoproteins except HDL, and determining the amount of hydrogen peroxide or reductive co-enzyme formed therein.Type: GrantFiled: November 2, 1995Date of Patent: November 25, 1997Assignee: Kyowa Medex Co., Ltd.Inventors: Kazuhito Miyauchi, Akira Miike, Eiko Shutoh, Hiroyuki Sugiuchi, Tetsumi Irie, Kaneto Uekama, Susumu Ohsawa