Abstract: Provided is a detection method employing a detection instrument having a plurality of wells at a surface thereof, the method including depositing a detection object, preferably in coexistence with a specific holding substance, onto the inner walls of the wells of the detection instrument; bringing a reaction solution into contact with the inner walls of the wells of the detection instrument on which the detection object has been deposited, the reaction solution containing a substance reactive with the detection object; and detecting a signal generated through this contact, to thereby evaluate the detection object. In this method, a liquid phase reaction can be efficiently performed in the detection instrument for carrying out a biological reaction (e.g., a microarray) so that samples contained in the wells are not mixed with one another, and that air bubbles are prevented from being generated in the wells.

Abstract: There are provided a) a detection board having numerous wells on a surface thereof, b) a detection set including the detection board and an element serving as well sealing means, and c) a method of using the detection set b), which includes injecting, into each of the wells of the detection board, an element for detecting a liquid phase reaction, sealing the element into the well with the well sealing means, and detecting the liquid phase reaction in the well. Thus, there is provided means for efficiently performing a liquid phase reaction on the board in a manner similar to that of an existing microarray technique.