Patents by Inventor Kazunori Okano
Kazunori Okano has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20150231635Abstract: In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.Type: ApplicationFiled: March 6, 2015Publication date: August 20, 2015Applicant: On-Chip Cellomics ConsortiumInventors: Kazunori Okano, Kenji Yasuda
-
Patent number: 8828679Abstract: Disclosed is a method for detecting a biological signal of a three-dimensional cell culture construct. The method for detecting a biological signal of a three-dimensional cell culture construct includes: providing a three-dimensional cell culture construct that contains at least two cell layers laminated to each other and a sensor particle capable of detecting a biological signal; and observing the sensor particle optically. Preferably, the three-dimensional cell culture construct contains an extracellular matrix including a combination of a protein or polymer having an RGD sequence and a protein or polymer that interacts with the protein or polymer having the RGD sequence, or a combination of a protein or polymer that is positively charged and a protein or polymer that is negatively charged.Type: GrantFiled: November 10, 2009Date of Patent: September 9, 2014Assignee: Japan Science and Technology AgencyInventors: Michiya Matsusaki, Mitsuru Akashi, Kazunori Okano
-
Publication number: 20130252848Abstract: In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.Type: ApplicationFiled: January 31, 2013Publication date: September 26, 2013Applicants: KANAGAWA ACADEMY OF SCIENCE AND TECHNOLOGY, Onchip Cellomics ConsortiumInventors: Kazunori Okano, Kenji Yasuda
-
Patent number: 8361412Abstract: A cell sorting device using an inexpensive chip capable of being exchanged for each sample. The chip includes: a first flow path allowing buffer fluid containing cells to flow down; second and third flow paths which put the first flow path therebetween and allow buffer fluid not containing cells to flow down; a fourth flow path which allows the buffer fluid as a single flow path formed by joining the buffer fluids in the other three flow paths; a cell detecting region for detecting cells flowing with the buffer fluid down the fourth flow path; and a cell sorting region for sorting the cells according to a type of the cells detected. The first to fourth flow paths are cascaded, are supplied with the buffer fluid from reservoirs with the same fluid level, and have substantially the same width or cross-section area.Type: GrantFiled: December 27, 2005Date of Patent: January 29, 2013Assignees: Japan Science and Technology Agency, Onchip Biotechnologies, Inc.Inventors: Kenji Yasuda, Akihiro Hattori, Kazunori Okano
-
Patent number: 8317847Abstract: A biostimulation apparatus of the present invention includes a laser oscillator for oscillating an ultra short pulsed laser beam and an optical system for focusing the ultra short pulsed laser beam, wherein the ultra short pulsed laser beam is focused by the optical system at a target portion of a living subject to cause the target portion to be irradiated with the laser beam to stimulate an acupuncture point, and wherein the target portion is either the acupuncture point or its periphery.Type: GrantFiled: September 18, 2008Date of Patent: November 27, 2012Assignees: Japan Science and Technology Agency, National University Corporation Kobe UniversityInventors: Yoichiro Hosokawa, Hiroshi Masuhara, Kazunori Okano, Yutaka Takaoka, Mika Ohta, Akihiko Ito
-
Publication number: 20110217726Abstract: Disclosed is a method for detecting a biological signal of a three-dimensional cell culture construct. The method for detecting a biological signal of a three-dimensional cell culture construct includes: providing a three-dimensional cell culture construct that contains at least two cell layers laminated to each other and a sensor particle capable of detecting a biological signal; and observing the sensor particle optically. Preferably, the three-dimensional cell culture construct contains an extracellular matrix including a combination of a protein or polymer having an RGD sequence and a protein or polymer that interacts with the protein or polymer having the RGD sequence, or a combination of a protein or polymer that is positively charged and a protein or polymer that is negatively charged.Type: ApplicationFiled: November 10, 2009Publication date: September 8, 2011Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Michiya Matsusaki, Mitsuru Akashi, Kazunori Okano
-
Patent number: 7964388Abstract: A device is provided for a chemical reaction between molecules immobilized on a solid phase and molecules in a solution. A chemical analysis device is also provided to capture molecules in the solution and subsequently measure the captured molecules. Reaction efficiency and sample throughput are thereby improved. The chemical reaction and chemical analysis devices use a microfluidic device channel as a reaction vessel. The channel is provided with a particular molecule immobilized on an interior surface of the channel with an obstacle positioned against the flow. In a typical reaction vessel having an enzyme immobilized in the capillary's interior surface and glass beads as obstacles, a reaction solution can move either in one direction or back and forth to react with the immobilized enzyme. The flow of the reaction solution is not laminar such that a reaction between the particular molecule and the reaction solution proceeds at high efficiency.Type: GrantFiled: December 30, 2009Date of Patent: June 21, 2011Assignee: Hitachi, Ltd.Inventors: Yoshinobu Kohara, Kazunori Okano, Hideyuki Noda
-
Patent number: 7846717Abstract: A microchamber including a glass substrate which is transparent to a specific wavelength, an absorbent region which absorbs the specific wavelength, and a melting substance region which does not absorb the specific wavelength, is solid at room temperature and melts when heated, which regions are layered on the glass substrate. The absorbent region, is irradiated with a focused light beam of the specific wavelength and locally heated in the vicinity of the converging rays, so that the melting substance region is locally melted at a portion adjacent to the absorbent region, thereby forming a cavity as the focused light beam moves. Accordingly, the shape of the microchamber can be arbitrarily changed in accordance with the process of cell culture.Type: GrantFiled: August 26, 2003Date of Patent: December 7, 2010Assignee: Japan Science and Technology AgencyInventors: Kenji Yasuda, Takanori Ichiki, Kazunori Okano
-
Publication number: 20100280580Abstract: A biostimulation apparatus of the present invention includes a laser oscillator for oscillating an ultra short pulsed laser beam and an optical system for focusing the ultra short pulsed laser beam, wherein the ultra short pulsed laser beam is focused by the optical system at a target portion of a living subject to cause the target portion to be irradiated with the laser beam to stimulate an acupuncture point, and wherein the target portion is either the acupuncture point or its periphery.Type: ApplicationFiled: September 18, 2008Publication date: November 4, 2010Inventors: Yoichiro Hosokawa, Hiroshi Masuhara, Kazunori Okano, Yutaka Tkaoka, Mika Ohta, Akihiko Ito
-
Publication number: 20100184237Abstract: A chemical reaction device is provided for a chemical reaction between molecules immobilized on a solid phase and molecules in a solution, and a chemical analysis device is also provided to capture molecules in the solution by molecules immobilized on the solid phase through a chemical reaction and subsequent measurement of the captured molecules. Reaction efficiency as well as sample throughput are thereby improved. The chemical reaction device and the chemical analysis device use a channel of a microfluidic device for a reaction vessel, and at least a particular molecule is immobilized on an interior surface and a fixed structure or a non-fixed obstacle against a flow is provided in the channel.Type: ApplicationFiled: December 30, 2009Publication date: July 22, 2010Inventors: Yoshinobu Kohara, Kazunori Okano, Hideyuki Noda
-
Publication number: 20100105104Abstract: The present invention provides a method, a chip device, and a system for quantitatively measuring intercellularly and intracellularly-localized mRNA and protein without loss of local space information. In the present invention, cell content is trapped atop a substrate or an electrode in the form of a two-dimensional projection of a cell. Accordingly, electrophoretic force is used, or the cellular moisture content is instantaneously evaporated and immobilized. mRNA immobilized to a two-dimensional surface is identified with a labeled probe which hybridizes to the mRNA.Type: ApplicationFiled: March 5, 2007Publication date: April 29, 2010Applicant: On-Chip Cellomics ConsortiumInventors: Kazunori Okano, Kenji Yasuda
-
Patent number: 7666662Abstract: A chemical reaction device is provided for a chemical reaction between molecules immobilized on a solid phase and molecules in a solution, and a chemical analysis device is also provided to capture molecules in the solution by molecules immobilized on the solid phase through a chemical reaction and subsequent measurement of the captured molecules. Reaction efficiency as well as sample throughput are thereby improved. The chemical reaction device and the chemical analysis device use a channel of a microfluidic device for a reaction vessel, and at least a particular molecule is immobilized on an interior surface and a fixed structure or a non-fixed obstacle against a flow is provided in the channel.Type: GrantFiled: May 26, 2004Date of Patent: February 23, 2010Assignee: Hitachi, Ltd.Inventors: Yoshinobu Kohara, Kazunori Okano, Hideyuki Noda
-
Publication number: 20100035278Abstract: The present invention provides a biological substance analysis chip capable of quantitative measurement in a minute region through immobilization of a biological substance trapping probe to a substrate surface in a more uniform density, a biological substance analysis kit that uses said chip, and a biological substance analysis method that uses the above.Type: ApplicationFiled: February 28, 2007Publication date: February 11, 2010Applicant: On-Chip Cellomics ConsortiumInventors: Kazunori Okano, Kenji Yasuda
-
Publication number: 20100021933Abstract: In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.Type: ApplicationFiled: May 26, 2009Publication date: January 28, 2010Inventors: Kazunori OKANO, Kenji Yasuda
-
Publication number: 20100018862Abstract: In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.Type: ApplicationFiled: May 26, 2009Publication date: January 28, 2010Inventors: Kazunori OKANO, Kenji Yasuda
-
Publication number: 20100016568Abstract: In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.Type: ApplicationFiled: May 26, 2009Publication date: January 21, 2010Inventors: Kazunori Okano, Kenji Yasuda
-
Publication number: 20100016569Abstract: In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.Type: ApplicationFiled: May 26, 2009Publication date: January 21, 2010Inventors: Kazunori Okano, Kenji Yasuda
-
Publication number: 20090325215Abstract: In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.Type: ApplicationFiled: May 26, 2009Publication date: December 31, 2009Inventors: Kazunori Okano, Kenji Yasuda
-
Publication number: 20090298717Abstract: There is provided means for analyzing organism-related molecules, dealing with multi item analysis, that are captured according to probe species, and for collecting according to the probe species. A magnetic micro-particle array is fixed with magnets that are configured with magnetic micro-particles in a capillary and with an array of glass beads to which DNA probes of different types from each other are immobilized. A syringe pump and a cross valve are operated to circulate a sample solution in the magnetic micro-particle array, which is reacted with probe DNAs on a glass bead with a probe. Subsequently, a washing solution is introduced to wash inside of the capillary. Next, respective beads are measured for fluorescence intensities. Furthermore a particular bead is collected based on results of fluorescence measurement. Target molecules captured on a surface of the collected bead may be separated by heat-denaturation, which then may be subjected to next analysis.Type: ApplicationFiled: June 18, 2009Publication date: December 3, 2009Inventors: Yoshinobu KOHARA, Kazunori OKANO, Hideyuki NODA
-
Patent number: 7597850Abstract: A droplet operation device has a substrate with light transmissibility and having been subjected to a water repellent treatment, a way for supplying droplets onto the substrate, a way for transporting the droplets on the water repellent substrate, and a way for measuring the state of the droplets. The droplet operation device includes the light transmittable substrate, solvent feeding ports for leading reagent or specimen droplets onto the substrate, optical measuring units, and electric field application units. As a result, the inside of the droplets can be observed with a microscope, and results of the reaction of the droplets against other droplets can be measured and classified by stopping and moving the droplets in any direction.Type: GrantFiled: August 26, 2003Date of Patent: October 6, 2009Inventors: Kenji Yasuda, Takanori Ichiki, Kazunori Okano