Patents by Inventor Kazuya Ishige
Kazuya Ishige has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240115592Abstract: The present invention relates to a PGC-1? expression promoting agent, a muscle building agent, or a mitochondria activating agent, which comprises at least one pyrimidine nucleotide or a precursor thereof as an active ingredient.Type: ApplicationFiled: December 27, 2021Publication date: April 11, 2024Inventors: Kosuke NAKAGAWARA, Chieri TAKEUCHI, Kazuya ISHIGE
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Publication number: 20240041910Abstract: The present invention is a muscular atrophy inhibitor comprising at least one pyrimidine nucleotide or a precursor thereof as an active ingredient. Also, the present invention is a method for inhibiting muscular atrophy by administrating at least one pyrimidine nucleotide or a precursor thereof.Type: ApplicationFiled: December 27, 2021Publication date: February 8, 2024Inventors: Kosuke NAKAGAWARA, Chieri TAKEUCHI, Kazuya ISHIGE
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Patent number: 11485754Abstract: A lyophilized product of cyclic-di-AMP requires special production equipment and is thus not suitable for large-scale production. Crystals of cyclic-di-AMP free acid are unstable under severe conditions at 105° C. Then, the present invention addresses the problem of providing a cyclic-di-AMP crystal that can be easily acquired in a large amount and is very stable under the severe conditions at 105° C. Crystals of c-di-AMP sodium salt according to the present invention are extremely stable even under the severe conditions at 105° C. Further, the crystals of c-di-AMP sodium salt according to the present invention can be prepared in a large amount by a simple process including adjusting a c-di-AMP aqueous solution at pH 5.2-12.0 and then adding an organic solvent thereto.Type: GrantFiled: October 31, 2019Date of Patent: November 1, 2022Assignee: Yamasa CorporationInventors: Misaki Takamatsu, Ko Yoshida, Marie Matsunaga, Kazuya Ishige
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Publication number: 20220136023Abstract: In an enzymatic synthesis of 3?,3?-cGAMP, other types of cyclic dinucleotides, c-di-GMP and c-di-AMP, are produced as by-products. One problem to be solved in order to establish a practical method for enzymatic synthesis of 3?,3?-cGAMP is suppression of production of these other types of cyclic dinucleotides during the synthesis. As a result of intensive studies, the inventors of the present invention found a variation of 3?,3?-cGAMP synthase by which the production of c-di-GMP and c-di-AMP is suppressed, and established a 3?,3?-cGAMP enzymatic synthesis system using this variation of the enzyme to complete the present invention. This enzyme brings about significantly reduced production of c-di-GMP and c-di-AMP, compared to the wild-type 3?,3?-cGAMP synthase. Accordingly, a production method using this enzyme makes it possible to reduce the production of other types of cyclic dinucleotides in comparison to conventional enzymatic synthesis methods, and efficiently synthesize 3?,3?-cGAMP.Type: ApplicationFiled: February 28, 2020Publication date: May 5, 2022Inventors: Ko YOSHIDA, Kazuya ISHIGE
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Publication number: 20210371451Abstract: A lyophilized product of cyclic-di-AMP requires special production equipment and is thus not suitable for large-scale production. Crystals of cyclic-di-AMP free acid are unstable under severe conditions at 105° C. Then, the present invention addresses the problem of providing a cyclic-di-AMP crystal that can be easily acquired in a large amount and is very stable under the severe conditions at 105° C. Crystals of c-di-AMP sodium salt according to the present invention are extremely stable even under the severe conditions at 105° C. Further, the crystals of c-di-AMP sodium salt according to the present invention can be prepared in a large amount by a simple process including adjusting a c-di-AMP aqueous solution at pH 5.2-12.0 and then adding an organic solvent thereto.Type: ApplicationFiled: October 31, 2019Publication date: December 2, 2021Inventors: Misaki TAKAMATSU, Ko YOSHIDA, Marie MATSUNAGA, Kazuya ISHIGE
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Patent number: 10836783Abstract: Though 3?,5?-cyclic diadenylic acid was conventionally provided only as a freeze-dried product, a solid material other than a freeze-dried product and a manufacturing method thereof is provided. By a step of adding acid to an aqueous solution of 3?,5?-cyclic diadenylic acid so as to lower pH to 1 to 3, an inclusion compound of 3?,5?-cyclic diadenylic acid can be obtained. Said manufacturing method is an extremely simple and easy method and does not need a special machine or the like.Type: GrantFiled: June 25, 2019Date of Patent: November 17, 2020Assignee: Yamasa CorporationInventors: Hisaki Tanaka, Kazuya Ishige
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Patent number: 10787479Abstract: A crystal of free acid of 3?,5?-cyclic diguanylic acid containing no metal salt with cobalt, magnesium or the like is provided. A method is sought for obtaining said crystal in a large amount and with ease. By a manufacturing method comprising a step of adding acid to an aqueous solution of 3?,5?-cyclic diguanylic acid so as to lower pH to 1 to 3, crystals of 3?,5?-cyclic diguanylic acid can be obtained in a large amount with ease. Said crystals are free acid crystals which do not contain a metal salt with cobalt, magnesium or the like.Type: GrantFiled: June 14, 2019Date of Patent: September 29, 2020Assignee: Yamasa CorporationInventors: Hisaki Tanaka, Kazuya Ishige
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Publication number: 20190382426Abstract: Though 3?,5?-cyclic diadenylic acid was conventionally provided only as a freeze-dried product, a solid material other than a freeze-dried product and a manufacturing method thereof is provided. By a step of adding acid to an aqueous solution of 3?,5?-cyclic diadenylic acid so as to lower pH to 1 to 3, an inclusion compound of 3?,5?-cyclic diadenylic acid can be obtained. Said manufacturing method is an extremely simple and easy method and does not need a special machine or the like.Type: ApplicationFiled: June 25, 2019Publication date: December 19, 2019Inventors: Hisaki TANAKA, Kazuya ISHIGE
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Publication number: 20190315795Abstract: A crystal of free acid of 3?,5?-cyclic diguanylic acid containing no metal salt with cobalt, magnesium or the like is provided. A method is sought for obtaining said crystal in a large amount and with ease. By a manufacturing method comprising a step of adding acid to an aqueous solution of 3?,5?-cyclic diguanylic acid so as to lower pH to 1 to 3, crystals of 3?,5?-cyclic diguanylic acid can be obtained in a large amount with ease. Said crystals are free acid crystals which do not contain a metal salt with cobalt, magnesium or the like.Type: ApplicationFiled: June 14, 2019Publication date: October 17, 2019Inventors: Hisaki TANAKA, Kazuya ISHIGE
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Patent number: 9926542Abstract: A practical method for enzymatically synthesizing c-di-GMP with excellent productivity is provided. A diguanylate cyclase having physical and chemical characteristics (A) to (F): (A) catalytic action on reaction “2 GTP?c-di-GMP”; (B) a molecular weight of 19800±2000; (C) an optimum pH of 7.3 to 9.4; (D) an optimum temperature of 35 to 60° C.; (E) thermal stability as the remaining activity of 90% or higher after heated for 60 minutes under conditions of 50° C. and pH7.8; and (F) the presence of GGDEF (SEQ ID NO:26) domain and the lack of amino acid sequence KXXD (SEQ ID NO:23) in the i-site.Type: GrantFiled: April 19, 2017Date of Patent: March 27, 2018Assignee: Yamasa CorporationInventors: Kaori Tanabe, Kazuya Ishige
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Publication number: 20170240871Abstract: A practical method for enzymatically synthesizing c-di-GMP with excellent productivity is provided. A diguanylate cyclase having physical and chemical characteristics (A) to (F): (A) catalytic action on reaction “2 GTP?c-di-GMP”; (B) a molecular weight of 19800±2000; (C) an optimum pH of 7.3 to 9.4; (D) an optimum temperature of 35 to 60° C.; (E) thermal stability as the remaining activity of 90% or higher after heated for 60 minutes under conditions of 50° C. and pH7.8; and (F) the presence of GGDEF (SEQ ID NO:26) domain and the lack of amino acid sequence KXXD (SEQ ID NO:23) in the i-site.Type: ApplicationFiled: April 19, 2017Publication date: August 24, 2017Inventors: Kaori TANABE, Kazuya ISHIGE
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Patent number: 9650615Abstract: A practical method for enzymatically synthesizing c-di-GMP with excellent productivity is provided. A diguanylate cyclase having physical and chemical characteristics (A) to (F): (A) catalytic action on reaction “2 GTP?c-di-GMP”; (B) a molecular weight of 19800±2000; (C) an optimum pH of 7.3 to 9.4; (D) an optimum temperature of 35 to 60° C.; (E) thermal stability as the remaining activity of 90% or higher after heated for 60 minutes under conditions of 50° C. and pH7.8; and (F) the presence of GGDEF (SEQ ID NO:26) domain and the lack of amino acid sequence KXXD (SEQ ID NO:23) in the i-site.Type: GrantFiled: February 26, 2013Date of Patent: May 16, 2017Assignee: Yamasa CorporationInventors: Kaori Tanabe, Kazuya Ishige
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Publication number: 20170101432Abstract: A crystal of free acid of 3?,5?-cyclic diguanylic acid containing no metal salt with cobalt, magnesium or the like is provided. A method is sought for obtaining said crystal in a large amount and with ease. By a manufacturing method comprising a step of adding acid to an aqueous solution of 3?,5?-cyclic diguanylic acid so as to lower pH to 1 to 3, crystals of 3?,5?-cyclic diguanylic acid can be obtained in a large amount with ease. Said crystals are free acid crystals which do not contain a metal salt with cobalt, magnesium or the like.Type: ApplicationFiled: February 27, 2015Publication date: April 13, 2017Inventors: Hisaki TANAKA, Kazuya ISHIGE
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Publication number: 20170096439Abstract: Though 3?,5?-cyclic diadenylic acid was conventionally provided only as a freeze-dried product, a solid material other than a freeze-dried product and a manufacturing method thereof is provided. By a step of adding acid to an aqueous solution of 3?,5?-cyclic diadenylic acid so as to lower pH to 1 to 3, an inclusion compound of 3?,5?-cyclic diadenylic acid can be obtained. Said manufacturing method is an extremely simple and easy method and does not need a special machine or the like.Type: ApplicationFiled: March 12, 2015Publication date: April 6, 2017Inventors: Hisaki TANAKA, Kazuya ISHIGE
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Patent number: 9193958Abstract: The invention provides a method for producing 3?-phosphoadenosine 5?-phosphosulfate (PAPS), the method including subjecting ATP to sulfation and phosphorylation by use of adenosine 5?-triphosphate sulfurylase (ATPS) and adenosine 5?-phosphosulfate kinase (APSK), wherein an adenosine 5?-triphosphate (ATP) supply/regeneration system including adenosine 5?-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5?-diphosphate kinase (PNDK), and polyphosphate:AMP phosphotransferase (PAP), or an adenosine 5?-triphosphate (ATP) supply/regeneration system including adenosine 5?-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5?-diphosphate kinase (PNDK), and adenylate kinase (ADK) is employed instead of ATP.Type: GrantFiled: March 6, 2014Date of Patent: November 24, 2015Assignee: YAMASA CORPORATIONInventors: Kazuya Ishige, Toshitada Noguchi
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Publication number: 20150044724Abstract: A practical method for enzymatically synthesizing c-di-GMP with excellent productivity is provided. A diguanylate cyclase having physical and chemical characteristics (A) to (F): (A) catalytic action on reaction “2 GTP?c-di-GMP”; (B) a molecular weight of 19800±2000; (C) an optimum pH of 7.3 to 9.4; (D) an optimum temperature of 35 to 60° C.; (E) thermal stability as the remaining activity of 90% or higher after heated for 60 minutes under conditions of 50° C. and pH 7.8; and (F) the presence of GGDEF domain and the lack of amino acid sequence KXXD in the i-site.Type: ApplicationFiled: February 26, 2013Publication date: February 12, 2015Inventors: Kaori Tanabe, Kazuya Ishige
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Publication number: 20140206042Abstract: The invention provides a method for producing 3?-phosphoadenosine 5?-phosphosulfate (PAPS), the method including subjecting ATP to sulfation and phosphorylation by use of adenosine 5?-triphosphate sulfurylase (ATPS) and adenosine 5?-phosphosulfate kinase (APSK), wherein an adenosine 5?-triphosphate (ATP) supply/regeneration system including adenosine 5?-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5?-diphosphate kinase (PNDK), and polyphosphate:AMP phosphotransferase (PAP), or an adenosine 5?-triphosphate (ATP) supply/regeneration system including adenosine 5?-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5?-diphosphate kinase (PNDK), and adenylate kinase (ADK) is employed instead of ATP.Type: ApplicationFiled: March 6, 2014Publication date: July 24, 2014Applicant: Yamasa CorporationInventors: KAZUYA ISHIGE, TOSHITADA NOGUCHI
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Patent number: 8728789Abstract: The invention provides a method for producing 3?-phosphoadenosine 5?-phosphosulfate (PAPS), the method including subjecting ATP to sulfation and phosphorylation by use of adenosine 5?-triphosphate sulfurylase (ATPS) and adenosine 5?-phosphosulfate kinase (APSK), wherein an adenosine 5?-triphosphate (ATP) supply/regeneration system including adenosine 5?-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5?-diphosphate kinase (PNDK), and polyphosphate:AMP phosphotransferase (PAP), or an adenosine 5?-triphosphate (ATP) supply/regeneration system including adenosine 5?-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5?-diphosphate kinase (PNDK), and adenylate kinase (ADK) is employed instead of ATP.Type: GrantFiled: January 24, 2006Date of Patent: May 20, 2014Assignee: Yamasa CorporationInventors: Kazuya Ishige, Toshitada Noguchi
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Patent number: 7863438Abstract: The present invention provides a stable salt of 3?-phosphoadenosine 5?-phosphosulfate (PAPS) and a production method therefor. The present invention is directed to a stable salt of PAPS (amine salt), which is formed between PAPS and an amine compound, and to a method for producing a stable salt of PAPS, which includes adding an amine compound to an aqueous PAPS solution in an amount by mole equal to or greater than that of PAPS, and lyophilizing the resultant solution. The present invention has first realized production of a solid-form PAPS salt having considerably improved stability through a very simple technique. Since the thus-produced amine salt of PAPS is very stable, the salt can be stored or employed without taking much care about decomposition thereof at ambient temperature.Type: GrantFiled: November 16, 2006Date of Patent: January 4, 2011Assignee: Yamasa CorporationInventors: Kazuya Ishige, Takashi Kawakami, Toshitada Noguchi
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Publication number: 20090215114Abstract: The invention provides a method for producing 3?-phosphoadenosine 5?-phosphosulfate (PAPS), the method including subjecting ATP to sulfation and phosphorylation by use of adenosine 5?-triphosphate sulfurylase (ATPS) and adenosine 5?-phosphosulfate kinase (APSK), wherein an adenosine 5?-triphosphate (ATP) supply/regeneration system including adenosine 5?-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5?-diphosphate kinase (PNDK), and polyphosphate:AMP phosphotransferase (PAP), or an adenosine 5?-triphosphate (ATP) supply/regeneration system including adenosine 5?-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5?-diphosphate kinase (PNDK), and adenylate kinase (ADK) is employed instead of ATP.Type: ApplicationFiled: January 24, 2006Publication date: August 27, 2009Applicant: YAMASA CORPORATIONInventors: Kazuya Ishige, Toshitada Noguchi