Abstract: A method for determining a fluorescent substance in a broader concentration range with higher measurement accuracy is provided which is applicable to assay of enzyme activity in enzyme-label immunoassay, enzyme-label DNA hybridization, etc. A method for assaying enzyme activity is also provided which employs the above fluorescence measurement.

Abstract: An automated analyzer for automatically assaying a specific component in a sample by utilizing a top-opened reaction vessel containing a reagent capable of reacting with the specific component, and which is sealed with a seal foil on which a symbol for the reagent is shown: said analyzer comprising a seal foil breaker for breaking the seal foil, a sample dispenser for dispensing the sample to the vessel, and a detector for detecting a reaction signal caused by a reaction with the specific component and the reagent; a transfer device for transferring the reaction vessel and a symbol discriminator for discriminating the symbol shown on the seal foil, which is placed at a position on a transfer route of the reaction vessels transferred by the transfer device and at a position before the seal-foil breaker.

Abstract: An apparatus for selectively injecting a reagent into a reaction chamber from a plurality of reagent reservoirs disposed in a circular configuration includes a separate dispenser mounted on each reagent reservoir. A vertically extending rod having a horizontally extending lever connected thereto is positioned concentrically within the circular array of reservoirs and drive motors are provided for rotating and reciprocating the rod and lever to selectively engage and operate the desired dispenser. Each dispenser is provided with its own distribution tube which directs the reagent in the reservoir directly into a selected reaction chamber, thereby preventing cross contamination of the reagents.