Patents by Inventor Keegan Owsley
Keegan Owsley has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11940369Abstract: Aspects of the disclosure include a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) with a top-hat-shaped excitation beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion to generate an image of the sample from the detected fluorescence. In another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency (e.g., a horizontal extent), which is generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency and detected fluorescence is used to analyze the sample.Type: GrantFiled: June 26, 2023Date of Patent: March 26, 2024Assignee: BECTON, DICKINSON AND COMPANYInventors: Eric D. Diebold, Keegan Owsley, Jonathan Lin
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Patent number: 11940372Abstract: Aspects of the present disclosure include a method for sorting cells of a sample based on an image of a cell in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image mask of a cell from the sample and sorting the cell based on the generated image mask. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image mask of a cell in a sample in a flow stream and to sort the cell based on the generated image mask are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for generating an image mask and for determining one or more features of the cell are also provided.Type: GrantFiled: September 28, 2022Date of Patent: March 26, 2024Assignee: BECTON, DICKINSON AND COMPANYInventors: Jonathan Lin, Matthew Bahr, Keegan Owsley
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Publication number: 20240019354Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.Type: ApplicationFiled: September 27, 2023Publication date: January 18, 2024Inventors: Jonathan Lin, Keegan Owsley, Matthew Bahr
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Patent number: 11808690Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.Type: GrantFiled: January 11, 2023Date of Patent: November 7, 2023Assignee: BECTON, DICKINSON AND COMPANYInventors: Jonathan Lin, Keegan Owsley, Matthew Bahr
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Publication number: 20230349812Abstract: In one aspect, a system for performing flow cytometry is disclosed, which comprises a laser for generating laser radiation for illuminating a sample, at least one detector for detecting at least a portion of a radiation emanating from the sample in response to said illumination so as to generate a temporal signal corresponding to said detected radiation, and an analysis module for receiving said temporal signal and performing a statistical analysis of said signal based on a forward model to reconstruct an image of said sample.Type: ApplicationFiled: June 26, 2023Publication date: November 2, 2023Inventors: Eric Diebold, Keegan Owsley, Jonathan Lin, Matthew Bahr
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Publication number: 20230333002Abstract: In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency.Type: ApplicationFiled: June 26, 2023Publication date: October 19, 2023Inventors: Eric D. Diebold, Keegan Owsley, Jonathan Lin
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Patent number: 11774343Abstract: In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution. In some embodiments, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.Type: GrantFiled: July 27, 2021Date of Patent: October 3, 2023Assignee: BECTON, DICKINSON AND COMPANYInventors: Eric D. Diebold, Keegan Owsley, Jonathan Lin
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Publication number: 20230296494Abstract: Aspects of the present disclosure include methods for producing an output laser beam having two or more angularly deflected laser beams (e.g., for irradiating a sample in a flow stream) with a predetermined intensity profile. Systems for practicing the subject methods having a laser, an acousto-optic device, a radiofrequency generator and a controller for adjusting the amplitude of the radiofrequency drive signals to produce an output laser beam of angularly deflected laser beams with a predetermined intensity profile are also described.Type: ApplicationFiled: May 19, 2023Publication date: September 21, 2023Inventors: Eric D. Diebold, Keegan Owsley, Matthew Bahr
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Publication number: 20230288311Abstract: In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency.Type: ApplicationFiled: May 17, 2023Publication date: September 14, 2023Inventors: Eric D. Diebold, Keegan Owsley, Jonathan Lin
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Publication number: 20230258551Abstract: Disclosed herein include systems, devices, computer readable media, and methods for subsampling flow cytometric event data. First and second flow cytometric event data can be transformed into a lower-dimensional space, associated with a plurality of bins, and assigned to a first bin and a second bin. Subsampled flow cytometric event data comprising the first flow cytometric event data can be generated. The subsampled flow cytometric event data can comprise the second flow cytometric event data if the first bin and the second bin are different. The subsampled flow cytometric event data may not comprise the second flow cytometric event data if the first bin and the second bin are identical.Type: ApplicationFiled: April 26, 2023Publication date: August 17, 2023Inventors: Jonathan Lin, Keegan Owsley, David A. Roberts
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Publication number: 20230243736Abstract: A cell sorting system that automatically generates a sorting strategy based on examples of target events provided by an operator. The target events can be selected using measurements ranging from traditional flow cytometry measurements to derived measurements that are computationally expensive to complex measurements such as images.Type: ApplicationFiled: April 4, 2023Publication date: August 3, 2023Inventors: Jonathan Lin, Joseph T. Trotter, Keegan Owsley
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Publication number: 20230228668Abstract: Disclosed herein include embodiments of a system, a device, and a method for sorting a plurality cells of a sample. A plurality of raw images comprising pixels of complex values in a frequency space can be generated from a plurality of channels of fluorescence intensity data of fluorescence emissions of fluorophores, the fluorescence emissions being elicited by fluorescence imaging using radiofrequency-multiplexed excitation in a temporal space. Spectral unmixing can be performed on the raw images prior to a sorting decision being made.Type: ApplicationFiled: February 24, 2023Publication date: July 20, 2023Inventors: Matthew Bahr, Keegan Owsley
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Patent number: 11698334Abstract: Aspects of the present disclosure include methods for producing an output laser beam having two or more angularly deflected laser beams (e.g., for irradiating a sample in a flow stream) with a predetermined intensity profile. Systems for practicing the subject methods having a laser, an acousto-optic device, a radiofrequency generator and a controller for adjusting the amplitude of the radiofrequency drive signals to produce an output laser beam of angularly deflected laser beams with a predetermined intensity profile are also described.Type: GrantFiled: August 26, 2020Date of Patent: July 11, 2023Assignee: BECTON, DICKINSON AND COMPANYInventors: Eric D. Diebold, Keegan Owsley, Matthew Bahr
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Patent number: 11692926Abstract: In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency.Type: GrantFiled: May 16, 2022Date of Patent: July 4, 2023Assignee: BECTON, DICKINSON AND COMPANYInventors: Eric D. Diebold, Keegan Owsley, Jonathan Lin
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Patent number: 11674881Abstract: Disclosed herein include systems, devices, computer readable media, and methods for subsampling flow cytometric event data. First and second flow cytometric event data can be transformed into a lower-dimensional space, associated with a plurality of bins, and assigned to a first bin and a second bin. Subsampled flow cytometric event data comprising the first flow cytometric event data can be generated. The subsampled flow cytometric event data can comprise the second flow cytometric event data if the first bin and the second bin are different. The subsampled flow cytometric event data may not comprise the second flow cytometric event data if the first bin and the second bin are identical.Type: GrantFiled: June 21, 2022Date of Patent: June 13, 2023Assignee: BECTON, DICKINSON AND COMPANYInventors: Jonathan Lin, Keegan Owsley, David A. Roberts
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Patent number: 11662297Abstract: Aspects of the present disclosure include methods for flow cytometrically sorting a sample with particles, such as cells, based on order of identification. Methods according to certain embodiments include introducing the sample into a flow cytometer; flowing the introduced sample in a flow stream; irradiating the sample in the flow stream with a light source; detecting light from cells in the sample flowing in the flow stream; identifying phenotypes of cells in the sample flowing in the flow stream based on one or more data signals generated from the detected light; and dynamically sorting into partitions cells of the sample that have a phenotype of a collection of predetermined phenotypes based on order of identification. Systems for practicing the subject methods are also provided. Non-transitory computer readable storage mediums are also described.Type: GrantFiled: April 9, 2021Date of Patent: May 30, 2023Assignee: BECTON, DICKINSON AND COMPANYInventors: David Alexander Roberts, Lingjie Wei, Peter Mage, Keegan Owsley
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Publication number: 20230152208Abstract: Aspects of the present disclosure include methods for dynamic real-time adjustment of data acquisition parameters of a particle analyzer. Methods according to certain embodiments include detecting light from a particle of a sample in a flow stream irradiated with a light source, generating an image of the particle based on the detected light and automatically adjusting a data acquisition parameter of the particle analyzer in response to a modulated visualization parameter for the image of the particle. Systems (e.g., particle analyzers) having a light source and a light detection system that includes an imaging photodetector and processor with memory having instructions for practicing the subject methods are also described. Non-transitory computer readable storage medium is also provided.Type: ApplicationFiled: November 10, 2022Publication date: May 18, 2023Inventors: Keegan Owsley, Christopher J. Wolf
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Publication number: 20230144214Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.Type: ApplicationFiled: January 11, 2023Publication date: May 11, 2023Inventors: Jonathan Lin, Keegan Owsley, Matthew Bahr
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Patent number: 11644407Abstract: A cell sorting system that automatically generates a sorting strategy based on examples of target events provided by an operator. The target events can be selected using measurements ranging from traditional flow cytometry measurements to derived measurements that are computationally expensive to complex measurements such as images.Type: GrantFiled: December 8, 2021Date of Patent: May 9, 2023Assignee: BECTON, DICKINSON AND COMPANYInventors: Jonathan Lin, Joseph Trotter, Keegan Owsley
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Patent number: 11625829Abstract: Disclosed herein include embodiments of a system, a device, and a method for sorting a plurality cells of a sample. A plurality of raw images comprising pixels of complex values in a frequency space can be generated from a plurality of channels of fluorescence intensity data of fluorescence emissions of fluorophores, the fluorescence emissions being elicited by fluorescence imaging using radiofrequency-multiplexed excitation in a temporal space. Spectral unmixing can be performed on the raw images prior to a sorting decision being made.Type: GrantFiled: April 6, 2022Date of Patent: April 11, 2023Assignee: Becton, Dickinson and CompanyInventors: Matthew Bahr, Keegan Owsley