Patents by Inventor Keiichi Yokoyama
Keiichi Yokoyama has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8093346Abstract: Provided is a method for efficiently producing a 3-amino-4-hydroxybenzoic acid-type compound by culturing a coryneform bacterium that has a gene encoding a mutated aspartokinase not subject to feedback inhibition, and that is transformed with a recombinant vector containing a DNA encoding a protein having an activity to form 3-amino-4-hydroxybenzoic acid from dihydroxyacetone phosphate and aspartate semialdehyde.Type: GrantFiled: January 3, 2011Date of Patent: January 10, 2012Assignee: Ajinomoto Co., Inc.Inventors: Mototaka Suzuki, Keiichi Yokoyama, Yoshimi Kikuchi
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Patent number: 8005904Abstract: To provide a user with better experience of cooperation between an electronic business card processing program and a communication program when exchanging electronic business cards. Electronic business cards are stored in a DB server, which is one of storages for a communication program, as well as an electronic business card local file. Thus, even if a PC is a company's PC, for example, the electronic business cards can be viewed by accessing the DB server from another PC or the like outside an office. Further, the electronic business card processing program operates with the communication program as backend, however, the electronic business card processing program may not be installed on a PC in some cases. Even in such a case, the communication program alone can display received electronic business cards that are stored in the communication program local file on the screen of a display.Type: GrantFiled: June 29, 2006Date of Patent: August 23, 2011Assignee: Microsoft CorporationInventors: Keiichi Yokoyama, Yasuhiko Mori, Makoto Fukuda, Yoshiaki Hara, Rajesh Ramanathan, Christopher C. Yu, Fumiaki Yagi
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Patent number: 7972829Abstract: The present invention relates to a process for secretory production of a foreign protein, in particular, transglutaminase by a coryneform bacterium. According to the present invention, a process is provided for the secretory production of a foreign protein, in particular, transglutaminase, by making a coryneform bacterium to produce an industrially useful foreign protein, in particular, transglutaminase and efficiently release the product extracellularly (i.e., secretory production).Type: GrantFiled: February 26, 2010Date of Patent: July 5, 2011Assignee: Ajinomoto Co., Inc.Inventors: Yoshimi Kikuchi, Masayo Date, Yukiko Umezawa, Keiichi Yokoyama, Hiroshi Matsui
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Publication number: 20110137007Abstract: Provided is a method for efficiently producing a 3-amino-4-hydroxybenzoic acid-type compound by culturing a coryneform bacterium that has a gene encoding a mutated aspartokinase not subject to feedback inhibition, and that is transformed with a recombinant vector containing a DNA encoding a protein having an activity to form 3-amino-4-hydroxybenzoic acid from dihydroxyacetone phosphate and aspartate semialdehyde.Type: ApplicationFiled: January 3, 2011Publication date: June 9, 2011Inventors: Mototaka Suzuki, Keiichi Yokoyama, Yoshimi Kikuchi
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Publication number: 20110073755Abstract: Molecules of a specific species can be selectively excited among molecules of a plurality of species that show only a slight difference of mass. Energy levels can be displayed on a graph where the horizontal axis indicates excitation energy. Assume an instance where an electromagnetic wave showing a comb-shaped spectrum having a plurality of narrow bands as indicated by P1 through P14 and tuning with excitation energies corresponding to the rotational levels of molecule X is irradiated onto the molecule X. The molecule X can sequentially make transitions to higher energy levels by using an electromagnetic wave showing such a comb-shaped spectrum. The energy levels of molecule Y are not synchronized with the comb-shaped spectrum. The two ranges of Y4 through Y7 and Y12 through Y15 operate as gates and the molecule Y cannot make transition from a rotational level to another when a gate is found between them.Type: ApplicationFiled: September 22, 2010Publication date: March 31, 2011Applicant: JAPAN ATOMIC ENERGY AGENCYInventors: Keiichi Yokoyama, Leo Matsuoka, Hiroshi Akagi, Tatsuya Kasajima, Masaaki Tsubouchi, Atsushi Yokoyama
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Publication number: 20110064847Abstract: A method of denaturing a protein by treating the protein with a protein glutaminase and a transglutaminase, a food containing a protein having been denatured with these enzymes, and an enzyme preparation for denaturing a protein which contains these enzymes. A protein is denatured by adding protein glutaminase and transglutaminase to the protein substantially at the same timing, or adding protein glutaminase to the protein before the transglutaminase acts on the protein, or controlling the quantitative ratio of protein glutaminase to transglutaminase, by which a protein is treated, to a definite level.Type: ApplicationFiled: September 14, 2010Publication date: March 17, 2011Applicants: AJINOMOTO CO., INC., Amano Enzyme Inc.Inventors: Noriko Miwa, Nobuhisa Shimba, Mina Nakamura, Eiichiro Suzuki, Keiichi Yokoyama, Hiroyuki Nakagoshi, Fumiyuki Hirose, Hiroaki Sato
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Publication number: 20100297729Abstract: The present invention relates to a process for secretory production of a foreign protein, in particular, transglutaminase by a coryneform bacterium. According to the present invention, a process is provided for the secretory production of a foreign protein, in particular, transglutaminase, by making a coryneform bacterium to produce an industrially useful foreign protein, in particular, transglutaminase and efficiently release the product extracellularly (i.e., secretory production).Type: ApplicationFiled: February 26, 2010Publication date: November 25, 2010Applicant: AJINOMOTO CO., INCInventors: Yoshimi Kikuchi, Masayo Date, Yukiko Umezawa, Keiichi Yokoyama, Hiroshi Matsui
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Publication number: 20100203314Abstract: A processed fiber product having excellent strength, water-absorbability and washing durability can be produced by attaching a partially hydrolyzed product of a wheat protein to a fiber and then allowing a transglutaminase to act on the fiber.Type: ApplicationFiled: April 23, 2010Publication date: August 12, 2010Applicant: Ajinomoto Co., Inc.Inventors: Mitsuo Ueda, Keiichi Yokoyama, Noriki Nio
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Publication number: 20100159560Abstract: The present invention provides a neutral metalloprotease from actinomycetes which selectively cleaves a pro-structure part of a microbial protransglutaminase and a gene encoding said neutral metalloprotease. An active microbial transglutaminase having the pro-structure part cleaved can be obtained by culturing a microorganism into which a gene encoding the neutral metalloprotease from actinomycetes according to the present invention has been introduced, where by producing the neutral metalloprotease from actinomycetes, and reacting it on a microbial protransglutaminase.Type: ApplicationFiled: March 1, 2010Publication date: June 24, 2010Inventors: Yukiko Umezawa, Keiichi Yokoyama, Yoshimi Kikuchi, Masayo Date, Norimasa Onishi
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Publication number: 20100143970Abstract: A transglutaminase protein which is mutated to have improved heat resistance and/or pH stability. A mutation is introduced into WT transglutaminase at a cysteine residue capable of forming a disulfide bond (SS bond).Type: ApplicationFiled: August 14, 2009Publication date: June 10, 2010Inventors: Keiichi Yokoyama, Mototaka Suzuki, Tatsuki Kashiwagi, Eiichiro Suzuki, Masayo Date, Seiichi Taguchi
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Patent number: 7723067Abstract: The present invention relates to a process for secretory production of a foreign protein, in particular, transglutaminase by a coryneform bacterium. According to the present invention, a process is provided for the secretory production of a foreign protein, in particular, transglutaminase, by making a coryneform bacterium to produce an industrially useful foreign protein, in particular, transglutaminase and efficiently release the product extracellularly (i.e., secretory production).Type: GrantFiled: April 1, 2002Date of Patent: May 25, 2010Assignee: Ajinomoto Co., Inc.Inventors: Yoshimi Kikuchi, Masayo Date, Yukiko Umezawa, Keiichi Yokoyama, Hiroshi Matsui
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Patent number: 7704707Abstract: The present invention provides a neutral metalloprotease from actinomycetes which selectively cleaves a pro-structure part of a microbial protransglutaminase and a gene encoding said neutral metalloprotease. An active microbial transglutaminase having the pro-structure part cleaved can be obtained by culturing a microorganism into which a gene encoding the neutral metalloprotease from actinomycetes according to the present invention has been introduced, where by producing the neutral metalloprotease from actinomycetes, and reacting it on a microbial protransglutaminase.Type: GrantFiled: September 6, 2005Date of Patent: April 27, 2010Assignee: Ajinomoto Co., Inc.Inventors: Yukiko Umezawa, Keiichi Yokoyama, Yoshimi Kikuchi, Masayo Date, Norimasa Onishi
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Publication number: 20090216839Abstract: To provide a user with better experience of cooperation between an electronic business card processing program and a communication program when exchanging electronic business cards. Electronic business cards are stored in a DB server, which is one of storages for a communication program, as well as an electronic business card local file. Thus, even if a PC is a company's PC, for example, the electronic business cards can be viewed by accessing the DB server from another PC or the like outside an office. Further, the electronic business card processing program operates with the communication program as back-end, however, the electronic business card processing program may not be installed on a PC in some cases. Even in such a case, the communication program alone can display received electronic business cards that are stored in the communication program local file on the screen of a display.Type: ApplicationFiled: June 29, 2006Publication date: August 27, 2009Inventors: Keiichi Yokoyama, Yasuhiko Mori, Makoto Fukuda, Yoshiaki Hara, Rajesh Ramanathan, Christopher C. Yu, Fumiaki Yagi
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Patent number: 7553650Abstract: The present invention relates to a method for designing and preparing mutant transglutaminases on the basis of the three-dimensional structure of MTG derived from Streptoverticillium mobaraense (MTG), and the mutant MTG thus prepared. The present invention provides a method for modifying MTG on the basis of the three-dimensional structure, and transglutaminase having reactivity on the substrate improved by the method. In the present invention, the binding site of MTG for the substrate is extrapolated based on the three-dimensional structure obtained by X-ray crystal structure analysis of MTG crystals, and the mutant transglutaminases are designed and produced by replacing, inserting or deleting amino acid residues positioned at the substrate-binding site of the transglutaminase.Type: GrantFiled: August 17, 2006Date of Patent: June 30, 2009Assignee: Ajinomoto Co., Inc.Inventors: Tatsuki Kashiwagi, Nobuhisa Shimba, Kohki Ishikawa, Ei-ichiro Suzuki, Keiichi Yokoyama, Kazuo Hirayama
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Publication number: 20080142354Abstract: To make it easier to carbonize the processing target material to a desired carbonized condition and also to make installment within a limited installment space possible. A carbonizing apparatus includes a carbonizing furnace having a feeding portion for a processing target material, a takeout portion for a carbonized material, a blowout portion for combustion air and an exhausting portion for combustion exhaust gas and a stirring device capable of stirring the processing target material inside the carbonizing furnace. The blowout amount of the combustion air is adjustable. The apparatus is operable to carbonize the processing target material fed from the feeding portion, with stirring, spontaneously combusting and moving the material while moving this material toward the takeout portion and subsequently to take out the resultant carbonized material from the takeout portion.Type: ApplicationFiled: July 30, 2004Publication date: June 19, 2008Inventors: Keiichi Yokoyama, Masaaki Fujiwara, Sadatoshi Ueda, Yoshiaki Arai, Tatsushi Kudo, Shigeru Miyahara
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Patent number: 7252972Abstract: The object of the present invention is to provide a method of producing a heterologous protein by making a coryneform bacterium to produce and efficiently extracellularly secrete (secreto-production) an industrially useful heterologous protein. According to the present invention, a genetic construct is used where a gene sequence encoding an intended protein which is ligated to the downstream of a sequence encoding the signal peptide derived from a coryneform bacterium, the gene construct is introduced into a mutant coryneform bacterium which has a capacity of secreting the heterologous protein at least 2-fold higher than the wild type Corynebacterium glutamicum ATCC 13869, the mutant coryneform bacterium is cultured and the extracellularly released heterologous protein is recovered.Type: GrantFiled: September 30, 2003Date of Patent: August 7, 2007Assignee: Ajinomoto Co., Inc.Inventors: Yoshimi Kikuchi, Masayo Date, Yukiko Umezawa, Keiichi Yokoyama, Haruo Heima, Hiroshi Matsui
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Publication number: 20060275872Abstract: The present invention relates to a method for designing and preparing mutant transglutaminases on the basis of the three-dimensional structure of MTG derived from Streptoverticillium mobaraense (MTG), and the mutant MTG thus prepared. The present invention provides a method for modifying MTG on the basis of the three-dimensional structure, and transglutaminase having reactivity on the substrate improved by the method. In the present invention, the binding site of MTG for the substrate is extrapolated based on the three-dimensional structure obtained by X-ray crystal structure analysis of MTG crystals, and the mutant transglutaminases are designed and produced by replacing, inserting or deleting amino acid residues positioned at the substrate-binding site of the transglutaminase.Type: ApplicationFiled: August 17, 2006Publication date: December 7, 2006Applicant: Ajinomoto Co., Inc.Inventors: Tatsuki Kashiwagi, Nobuhisa Shimba, Kohki Ishikawa, Ei-ichiro Suzuki, Keiichi Yokoyama, Kazuo Hirayama
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Publication number: 20060167305Abstract: The invention provides a (meth)acrylate represented by the general formula (I) wherein X is a single bond or an alkylidene group having the general formula (II) wherein R3 and R4 are independently hydrogen atoms or alkyl groups having 1 to 4 carbon atoms, R1 is a hydrogen atom or a methyl group, R2 is an alkyl group having 1 to 4 carbon atoms, m is 0 or 1, and when m is 0, n is 3, and when m is 1, n is 0. The (meth)acrylate is obtained by reacting a cycloaliphatic ketone, an organomagnesium halide and a (meth)acrylic acid ester in the presence of an amine under the industrially feasible reaction conditions and operation according to the invention.Type: ApplicationFiled: October 4, 2005Publication date: July 27, 2006Applicant: HONSHU CHEMICAL INDUSTRY CO., LTD.Inventor: Keiichi Yokoyama
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Publication number: 20060019367Abstract: The present invention provides a neutral metalloprotease from actinomycetes which selectively cleaves a pro-structure part of a microbial protransglutaminase and a gene encoding said neutral metalloprotease. An active microbial transglutaminase having the pro-structure part cleaved can be obtained by culturing a microorganism into which a gene encoding the neutral metalloprotease from actinomycetes according to the present invention has been introduced, where by producing the neutral metalloprotease from actinomycetes, and reacting it on a microbial protransglutaminase.Type: ApplicationFiled: September 6, 2005Publication date: January 26, 2006Inventors: Yukiko Umezawa, Keiichi Yokoyama, Yoshimi Kikuchi, Masayo Date, Norimasa Onishi
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Publication number: 20060002564Abstract: A CD-R where a key generating program or the like is stored, and a USB memory where a public key is stored are delivered to a supplier from a receiver. A common key is generated by the supplier. Delivery data which is to be encrypted using the generated common key and a common key which is encrypted using the public key are stored in the CD-R by the supplier. The CD-R and the USB memory are delivered to the receiver by the supplier. The encrypted common key is decrypted by the receiver using a private key. The encrypted delivery data is decrypted by the receiver using the decrypted common key.Type: ApplicationFiled: July 5, 2005Publication date: January 5, 2006Inventors: Kenichi Aihara, Kazuhito Iizuka, Takashi Nihei, Keiichi Yokoyama, Hiroharu Yoshikawa, Yuuichi Uwaya