Patents by Inventor Keiji Nishida
Keiji Nishida has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240170517Abstract: A solid-state imaging element (1) according to the present disclosure includes a plurality of light-receiving pixels (11) arranged in a matrix inside a semiconductor layer (20). The light-receiving pixel (11) includes a pair of photoelectric conversion units, a first separation region (24), a second separation region (25), and a third separation region (26). The pair of photoelectric conversion units are disposed adjacent to each other. The first separation region (24) is disposed so as to surround the pair of photoelectric conversion units and is disposed so as to penetrate the semiconductor layer (20). The second separation region (25) is disposed between the pair of photoelectric conversion units and is disposed so as to penetrate the semiconductor layer (20). The third separation region (26) is disposed in a region surrounded by the first separation region (24) and is disposed from a light incident surface (20a) of the semiconductor layer (20) to a middle of the semiconductor layer (20).Type: ApplicationFiled: February 15, 2022Publication date: May 23, 2024Inventors: KEIJI NISHIDA, KOJI SEKIGUCHI
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Publication number: 20240132898Abstract: The invention provides a method for producing a plant cell modified at a targeted site of a double-stranded DNA, including (i) a step of providing a plant cell comprising the double-stranded DNA of interest, (ii) a step of providing a complex in which a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in the double-stranded DNA and a DNA glycosylase with sufficiently low reactivity with the double-stranded DNA are bound, (iii) a step of placing the complex in a condition under which the plant cell is transfected, (iv) a step of placing the transfected plant cell in a condition that induces modification of the targeted site, without cleaving at least one strand of the double-stranded DNA in the targeted site, and (v) a step of selecting a cell into which the complex has been introduced and/or a cell into which the modification has been introduced.Type: ApplicationFiled: February 25, 2022Publication date: April 25, 2024Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITYInventors: Keiji NISHIDA, Zenpei SHIMATANI, Ushio FUJIKURA
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Publication number: 20240117384Abstract: Provided is a method for altering a targeted site of a DNA in a cell, including a step of stimulating the cell with a factor inducing a DNA modifying enzyme endogenous to the cell, and bringing a complex of a nucleic acid sequence-recognizing module specifically binding to a target nucleotide sequence in a given DNA and a DNA modifying enzyme-binding module bonded to each other into contact with the DNA to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site.Type: ApplicationFiled: December 14, 2023Publication date: April 11, 2024Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITYInventors: Keiji NISHIDA, Akihiko KONDO, Takayuki ARAZOE, Shin YOSHIOKA
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Publication number: 20240093240Abstract: The invention provides a site-specific modification method of a DNA molecule that enables gene function manipulation. In particular, the invention provides a method for manipulating gene function by a site-specific action on a target DNA molecule, said method comprising a step for preparing a complex containing an Argonaute protein, a guide RNA and an additional sequence and a step for contacting the target DNA molecule with the complex, wherein the guide RNA is designed to guide the complex to a region containing the desired site in the target DNA molecule.Type: ApplicationFiled: December 28, 2021Publication date: March 21, 2024Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITYInventors: Keiji NISHIDA, Hitoshi MITSUNOBU
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Publication number: 20240006443Abstract: The present disclosure relates to a solid-state imaging device, an imaging device, and an electronic apparatus that are capable of suppressing generation of flare and also suppressing coloring caused by the flare with a simple configuration. A high refractive index layer is formed between a solid-state imaging element and a transparent protective substrate (glass substrate). When reflected light caused by diffracted light generated from an on-chip lens is reflected at an interface with the high refractive index layer, the reflected light is entirely reflected at a surface layer that is a transparent protective substrate and then the reflected light is sufficiently attenuated before being incident again. Consequently, flare and coloring caused by the flare are suppressed. The present disclosure is adaptable to an imaging device.Type: ApplicationFiled: July 7, 2023Publication date: January 4, 2024Inventor: KEIJI NISHIDA
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Patent number: 11845953Abstract: Provided is a method for altering a targeted site of a DNA in a cell, including a step of stimulating the cell with a factor inducing a DNA modifying enzyme endogenous to the cell, and bringing a complex of a nucleic acid sequence-recognizing module specifically binding to a target nucleotide sequence in a given DNA and a DNA modifying enzyme-binding module bonded to each other into contact with the DNA to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site.Type: GrantFiled: March 20, 2018Date of Patent: December 19, 2023Assignee: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITYInventors: Keiji Nishida, Akihiko Kondo, Takayuki Arazoe, Shin Yoshioka
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Publication number: 20230323335Abstract: The invention provides a miniaturized cytidine deaminase-containing complex for modifying DNA formed by combining a nucleic acid sequence recognition module and cytidine deaminase, wherein the nucleic acid sequence recognition module specifically binds to a target nucleotide sequence of double-stranded DNA, the cytidine deaminase is composed of an amino acid sequence composed of a region of amino acid residues at positions 30-150 of SEQ ID NO: 1, an ortholog thereof, an amino acid sequence having mutations of one or several amino acids therein, or an amino acid sequence having at least 90% similarity thereto, and the targeted site of the double-stranded DNA is modified.Type: ApplicationFiled: September 6, 2021Publication date: October 12, 2023Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITYInventors: Keiji NISHIDA, Ang LI, Hitoshi MITSUNOBU
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Patent number: 11728363Abstract: The present disclosure relates to a solid-state imaging device, an imaging device, and an electronic apparatus that are capable of suppressing generation of flare and also suppressing coloring caused by the flare with a simple configuration. A high refractive index layer is formed between a solid-state imaging element and a transparent protective substrate (glass substrate). When reflected light caused by diffracted light generated from an on-chip lens is reflected at an interface with the high refractive index layer, the reflected light is entirely reflected at a surface layer that is a transparent protective substrate and then the reflected light is sufficiently attenuated before being incident again. Consequently, flare and coloring caused by the flare are suppressed. The present disclosure is adaptable to an imaging device.Type: GrantFiled: August 8, 2019Date of Patent: August 15, 2023Assignee: SONY SEMICONDUCTOR SOLUTIONS CORPORATIONInventor: Keiji Nishida
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Patent number: 11718846Abstract: The invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and a nucleic acid base converting enzyme are linked, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.Type: GrantFiled: February 14, 2020Date of Patent: August 8, 2023Assignee: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITYInventors: Keiji Nishida, Akihiko Kondo, Satomi Kojima
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Publication number: 20230151376Abstract: The present invention provides an organelle transformant screening method. More specifically, the present invention provides an organelle transformant screening method utilizing inhibition of a lipid synthetic pathway.Type: ApplicationFiled: March 24, 2021Publication date: May 18, 2023Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITYInventors: Kenta KATAYAMA, Jun TERAMOTO, Keiji NISHIDA
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Publication number: 20230143387Abstract: An imaging device includes a plurality of imaging elements, wherein each of the plurality of imaging elements includes: a plurality of pixels containing impurities of a first conductivity type; an element separation wall surrounding the plurality of pixels and provided so as to penetrate a semiconductor substrate; an on-chip lens provided above a light receiving surface of the semiconductor substrate so as to be shared by the plurality of pixels; and a first separation portion provided in a region surrounded by the element separation wall and separating the plurality of pixels, the first separation portion is provided so as to extend in a thickness direction of the semiconductor substrate, and a first diffusion region containing impurities of a second conductivity type opposite to the first conductivity type is provided in a region positioned around the first separation portion and extending in the thickness direction of the semiconductor substrate.Type: ApplicationFiled: March 26, 2021Publication date: May 11, 2023Applicant: SONY SEMICONDUCTOR SOLUTIONS CORPORATIONInventors: Akira MATSUMOTO, Koichiro ZAITSU, Keiji NISHIDA, Mizuki NISHIDA, Kazutaka IZUKASHI, Daisuke ITO, Yasufumi MIYOSHI, Junpei YAMAMOTO, Yusuke TANAKA, Yasushi HAMAMOTO
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Publication number: 20230008784Abstract: Provided is a solid-state imaging device capable of suppressing color mixing between different colors while reducing the sensitivity difference between same colors. The solid-state imaging device includes: a plurality of photoelectric conversion units formed on a substrate to generate signal charges according to an amount of incident light; a microlens array including a microlens formed for a photoelectric conversion unit group including at least two or more adjacent photoelectric conversion units 21 to guide incident light to the photoelectric conversion unit group; a scatterer disposed on an optical path of the incident light collected by the microlens; and an inter-pixel light shielding portion including a groove formed between the photoelectric conversion unit of the photoelectric conversion unit group and the photoelectric conversion unit adjacent to the photoelectric conversion unit group and an insulating material filled in the groove.Type: ApplicationFiled: October 30, 2020Publication date: January 12, 2023Inventor: KEIJI NISHIDA
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Publication number: 20220259610Abstract: The present invention provides a method for selecting a promoter that functions in an organelle, the method comprising: (1) the step of preparing sequence information obtained by RNA sequencing analysis; (2) the step of mapping the sequence information prepared in the step (1) onto a sequence of organellar DNA; (3) the step of calculating the amount of change in RNA expression in each region based on the mapping information obtained in the step (2); (4) the step of selecting regions in which the amount of change obtained in the step (3) is within a range of preset reference values; and (5) the step of identifying a region, among the regions selected, as a promoter functioning in an organelle.Type: ApplicationFiled: March 6, 2020Publication date: August 18, 2022Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITYInventors: Kenta KATAYAMA, Keiji NISHIDA
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Patent number: 11220693Abstract: The present invention provides a method of modifying a targeted site of a double stranded DNA of a monocot cell, comprising a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in the given double stranded DNA and a nucleic acid base converting enzyme are bonded, with said double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into said targeted site, without cleaving at least one strand of said double stranded DNA in the targeted site, wherein the double stranded DNA is contacted with the complex by introducing a nucleic acid encoding the complex into the monocot cell.Type: GrantFiled: November 25, 2016Date of Patent: January 11, 2022Assignee: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITYInventors: Keiji Nishida, Zenpei Shimatani, Akihiko Kondo
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Publication number: 20210305302Abstract: The present disclosure relates to a solid-state imaging device, an imaging device, and an electronic apparatus that are capable of suppressing generation of flare and also suppressing coloring caused by the flare with a simple configuration. A high refractive index layer is formed between a solid-state imaging element and a transparent protective substrate (glass substrate). When reflected light caused by diffracted light generated from an on-chip lens is reflected at an interface with the high refractive index layer, the reflected light is entirely reflected at a surface layer that is a transparent protective substrate and then the reflected light is sufficiently attenuated before being incident again. Consequently, flare and coloring caused by the flare are suppressed. The present disclosure is adaptable to an imaging device.Type: ApplicationFiled: August 8, 2019Publication date: September 30, 2021Inventor: KEIJI NISHIDA
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Patent number: 11041169Abstract: The present invention provides a method for modifying a targeted site of a double-stranded DNA in a cell, the method including a step of bringing a complex in which a nucleic acid sequence-recognizing module that specifically binds to a selected target nucleotide sequence in a double-stranded DNA and a nucleic acid base converting enzyme or DNA glycosylase are linked, and a donor DNA containing an insertion sequence into contact with said double-stranded DNA, to substitute the targeted site with the insertion sequence, or insert the insertion sequence into said targeted site, without cleaving at least one strand of said double-stranded DNA in the targeted site.Type: GrantFiled: March 26, 2019Date of Patent: June 22, 2021Assignee: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITYInventor: Keiji Nishida
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Publication number: 20210171935Abstract: The present invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and DNA glycosylase with sufficiently low reactivity with a DNA having an unrelaxed double helix structure (unrelaxed DNA) are bonded, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.Type: ApplicationFiled: February 12, 2021Publication date: June 10, 2021Applicant: National University Corporation Kobe UniversityInventors: Keiji NISHIDA, Akihiko KONDO
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Patent number: 11001856Abstract: The present invention provides a method for modifying a targeted site of a double-stranded DNA in a cell, the method including a step of bringing a complex in which a nucleic acid sequence-recognizing module that specifically binds to a selected target nucleotide sequence in a double-stranded DNA and a nucleic acid base converting enzyme or DNA glycosylase are linked, and a donor DNA containing an insertion sequence into contact with said double-stranded DNA, to substitute the targeted site with the insertion sequence, or insert the insertion sequence into said targeted site, without cleaving at least one strand of said double-stranded DNA in the targeted site.Type: GrantFiled: March 26, 2019Date of Patent: May 11, 2021Assignee: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITYInventor: Keiji Nishida
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Patent number: 10920215Abstract: The present invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and DNA glycosylase with sufficiently low reactivity with a DNA having an unrelaxed double helix structure (unrelaxed DNA) are bonded, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.Type: GrantFiled: November 2, 2015Date of Patent: February 16, 2021Assignee: National University Corporation Kobe UniversityInventors: Keiji Nishida, Akihiko Kondo
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Publication number: 20210024906Abstract: The present invention provides a complex containing a nucleic acid sequence-recognizing module and a proteolysis tag, wherein the module is linked to the proteolysis tag, the module specifically binds to a target nucleotide sequence in a double stranded DNA, and the tag consists of (i) a peptide containing 3 hydrophobic amino acid residues at the C-terminal, or (ii) a peptide containing 3 amino acid residues at the C-terminal m wherein at least a part of the amino acid residues is substituted by serine.Type: ApplicationFiled: November 21, 2018Publication date: January 28, 2021Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITYInventor: Keiji NISHIDA