Patents by Inventor Keiko Kurosawa
Keiko Kurosawa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11629341Abstract: The purpose of the present invention is to provide a method for producing selenoneine that allows production of selenoneine at higher yields as compared with a conventional technology, and, therefore, enables selenoneine production on an industrial scale. This purpose can be achieved by a method for producing selenoneine, comprising the step of applying histidine and a selenium compound to a transformant that has a gene encoding an enzyme of (1) below introduced therein and that can overexpress the introduced gene, to obtain selenoneine. (1) An enzyme that catalyzes a reaction in which hercynylselenocysteine is produced from histidine and selenocysteine in the presence of S-adenosylmethionine and iron (II).Type: GrantFiled: May 13, 2021Date of Patent: April 18, 2023Assignee: KIKKOMAN CORPORATIONInventors: Keiichi Ichikawa, Yasutomo Shinohara, Seiichi Hara, Keiko Kurosawa, Yumiko Yamashita, Michiaki Yamashita
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Transformed fungus having enhanced ergothioneine productivity and method for producing ergothioneine
Patent number: 11186855Abstract: The purpose of the present invention is to provide an organism having an ergothioneine productivity that is capable of easily producing ergothioneine within a short period of time at a high yield, as compared with a conventional technology, and, therefore, enables ergothioneine production on an industrial scale. This purpose can be achieved by a transformed fungus into which a gene encoding enzyme (1) or genes encoding enzymes (1) and (2) have been inserted and in which the inserted gene(s) are overexpressed. (1) an enzyme catalyzing a reaction of synthesizing hercynyl cysteine sulfoxide from histidine and cysteine in the presence of S-adenosyl methionine, iron (II) and oxygen. (2) An enzyme catalyzing a reaction of synthesizing ergothioneine from hercynyl cysteine sulfoxide using pyridoxal 5?-phosphate as a coenzyme.Type: GrantFiled: April 27, 2020Date of Patent: November 30, 2021Assignee: KIKKOMAN CORPORATIONInventors: Seiichi Hara, Keiko Kurosawa, Keiichi Ichikawa -
Publication number: 20210317463Abstract: The purpose of the present invention is to provide a method for producing selenoneine that allows production of selenoneine at higher yields as compared with a conventional technology, and, therefore, enables selenoneine production on an industrial scale. This purpose can be achieved by a method for producing selenoneine, comprising the step of applying histidine and a selenium compound to a transformant that has a gene encoding an enzyme of (1) below introduced therein and that can overexpress the introduced gene, to obtain selenoneine. (1) An enzyme that catalyzes a reaction in which hercynylselenocysteine is produced from histidine and selenocysteine in the presence of S-adenosylmethionine and iron (II).Type: ApplicationFiled: May 13, 2021Publication date: October 14, 2021Applicant: KIKKOMAN CORPORATIONInventors: Keiichi ICHIKAWA, Yasutomo SHINOHARA, Seiichi HARA, Keiko KUROSAWA, Yumiko YAMASHITA, Michiaki YAMASHITA
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Patent number: 11028400Abstract: The purpose of the present invention is to provide a method for producing selenoneine that allows production of selenoneine at higher yields as compared with a conventional technology, and, therefore, enables selenoneine production on an industrial scale. This purpose can be achieved by a method for producing selenoneine, comprising the step of applying histidine and a selenium compound to a transformant that has a gene encoding an enzyme of (1) below introduced therein and that can overexpress the introduced gene, to obtain selenoneine. (1) An enzyme that catalyzes a reaction in which hercynylselenocysteine is produced from histidine and selenocysteine in the presence of S-adenosylmethionine and iron (II).Type: GrantFiled: June 17, 2016Date of Patent: June 8, 2021Assignee: KIKKOMAN CORPORATIONInventors: Keiichi Ichikawa, Yasutomo Shinohara, Seiichi Hara, Keiko Kurosawa, Yumiko Yamashita, Michiaki Yamashita
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TRANSFORMED FUNGUS HAVING ENHANCED ERGOTHIONEINE PRODUCTIVITY AND METHOD FOR PRODUCING ERGOTHIONEINE
Publication number: 20200318147Abstract: The purpose of the present invention is to provide an organism having an ergothioneine productivity that is capable of easily producing ergothioneine within a short period of time at a high yield, as compared with a conventional technology, and, therefore, enables ergothioneine production on an industrial scale. This purpose can be achieved by a transformed fungus into which a gene encoding enzyme (1) or genes encoding enzymes (1) and (2) have been inserted and in which the inserted gene(s) are overexpressed. (1) an enzyme catalyzing a reaction of synthesizing hercynyl cysteine sulfoxide from histidine and cysteine in the presence of S-adenosyl methionine, iron (II) and oxygen. (2) An enzyme catalyzing a reaction of synthesizing ergothioneine from hercynyl cysteine sulfoxide using pyridoxal 5?-phosphate as a coenzyme.Type: ApplicationFiled: April 27, 2020Publication date: October 8, 2020Applicant: KIKKOMAN CORPORATIONInventors: Seiichi HARA, Keiko KUROSAWA, Keiichi ICHIKAWA -
Transformed fungus having enhanced ergothioneine productivity and method for producing ergothioneine
Patent number: 10760107Abstract: The purpose of the present invention is to provide an organism having an ergothioneine productivity that is capable of easily producing ergothioneine within a short period of time at a high yield, as compared with a conventional technology, and, therefore, enables ergothioneine production on an industrial scale. This purpose can be achieved by a transformed fungus into which a gene encoding enzyme (1) or genes encoding enzymes (1) and (2) have been inserted and in which the inserted gene(s) are overexpressed. (1) an enzyme catalyzing a reaction of synthesizing hercynyl cysteine sulfoxide from histidine and cysteine in the presence of S-adenosyl methionine, iron (II) and oxygen. (2) An enzyme catalyzing a reaction of synthesizing ergothioneine from hercynyl cysteine sulfoxide using pyridoxal 5?-phosphate as a coenzyme.Type: GrantFiled: December 25, 2015Date of Patent: September 1, 2020Assignee: KIKKOMAN CORPORATIONInventors: Seiichi Hara, Keiko Kurosawa, Keiichi Ichikawa -
Transformed fungus having enhanced ergothioneine productivity and method for producing ergothioneine
Patent number: 10711290Abstract: The purpose of the present invention is to provide an organism having an ergothioneine productivity that is capable of easily producing ergothioneine within a short period of time at a high yield, as compared with a conventional technology, and, therefore, enables ergothioneine production on an industrial scale. This purpose can be achieved by a transformed fungus into which a gene encoding enzyme (1) or genes encoding enzymes (1) and (2) have been inserted and in which the inserted gene(s) are overexpressed. (1) an enzyme catalyzing a reaction of synthesizing hercynyl cysteine sulfoxide from histidine and cysteine in the presence of S-adenosyl methionine, iron (II) and oxygen. (2) An enzyme catalyzing a reaction of synthesizing ergothioneine from hercynyl cysteine sulfoxide using pyridoxal 5?-phosphate as a coenzyme.Type: GrantFiled: December 25, 2015Date of Patent: July 14, 2020Assignee: KIKKOMAN CORPORATIONInventors: Seiichi Hara, Keiko Kurosawa, Keiichi Ichikawa -
Publication number: 20180237815Abstract: The purpose of the present invention is to provide a method for producing selenoneine that allows production of selenoneine at higher yields as compared with a conventional technology, and, therefore, enables selenoneine production on an industrial scale. This purpose can be achieved by a method for producing selenoneine, comprising the step of applying histidine and a selenium compound to a transformant that has a gene encoding an enzyme of (1) below introduced therein and that can overexpress the introduced gene, to obtain selenoneine. (1) An enzyme that catalyzes a reaction in which hercynylselenocysteine is produced from histidine and selenocysteine in the presence of S-adenosylmethionine and iron (II).Type: ApplicationFiled: June 17, 2016Publication date: August 23, 2018Applicant: KIKKOMAN CORPORATIONInventors: Keiichi ICHIKAWA, Yasutomo SHINOHARA, Seiichi HARA, Keiko KUROSAWA, Yumiko YAMASHITA, Michiaki YAMASHITA
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TRANSFORMED FUNGUS HAVING ENHANCED ERGOTHIONEINE PRODUCTIVITY AND METHOD FOR PRODUCING ERGOTHIONEINE
Publication number: 20170321235Abstract: The purpose of the present invention is to provide an organism having an ergothioneine productivity that is capable of easily producing ergothioneine within a short period of time at a high yield, as compared with a conventional technology, and, therefore, enables ergothioneine production on an industrial scale. This purpose can be achieved by a transformed fungus into which a gene encoding enzyme (1) or genes encoding enzymes (1) and (2) have been inserted and in which the inserted gene(s) are overexpressed. (1) an enzyme catalyzing a reaction of synthesizing hercynyl cysteine sulfoxide from histidine and cysteine in the presence of S-adenosyl methionine, iron (II) and oxygen. (2) An enzyme catalyzing a reaction of synthesizing ergothioneine from hercynyl cysteine sulfoxide using pyridoxal 5?-phosphate as a coenzyme.Type: ApplicationFiled: December 25, 2015Publication date: November 9, 2017Applicant: KIKKOMAN CORPORATIONInventors: Seiichi HARA, Keiko KUROSAWA, Keiichi ICHIKAWA -
Publication number: 20120295252Abstract: A composition for analyzing nucleic acid that contains luciferase for which reactivity to dATP is equal to or less than 1/400 reactivity to ATP. A method for analyzing nucleic acid that comprises the use of the composition. A kit for analyzing nucleic acid comprising the composition. The method provides for an inexpensive, highly accurate and highly sensitive nucleic acid analysis that uses dATP instead of an expensive reagent having a low reactivity to DNA polymerase.Type: ApplicationFiled: June 20, 2011Publication date: November 22, 2012Applicant: Kikkoman CorporationInventors: Shigeya Suzuki, Yukako Kodama, Keiko Kurosawa, Eiry Kobatake, Masayasu Mie
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Patent number: 7989190Abstract: The present invention provides a firefly luciferase for inexpensive, highly accurate and highly sensitive nucleic acid analysis that uses dATP instead of an expensive reagent having low reactivity to DNA polymerase in the manner of dATP?S, a method of analyzing nucleic acid that uses that luciferase, and a kit for analyzing nucleic acid thereof. The present invention relates to a composition for analyzing nucleic acid that contains luciferase for which reactivity to dATP is equal to or less than 1/400 reactivity to ATP, a method of analyzing nucleic acid that comprises the use of that composition, and a kit for analyzing nucleic acid comprising that composition.Type: GrantFiled: November 19, 2008Date of Patent: August 2, 2011Assignee: Kikkoman CorporationInventors: Shigeya Suzuki, Yukako Kodama, Keiko Kurosawa
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Publication number: 20110003361Abstract: The present invention has an object of providing a novel fructosyl peptide oxidase having superior physicochemical properties such as stability that is useful as an enzyme for clinical diagnosis, and an object of providing a method for producing the fructosyl peptide oxidase. A novel fructosyl peptide oxidase having physicochemical properties useful as an enzyme for clinical diagnosis, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing a microorganism capable of producing the oxidase in a medium; and collecting the oxidase from the culture. Furthermore, a fructosyl peptide oxidase gene coding for a novel fructosyl peptide oxidase, recombinant DNA wherein the gene is inserted into vector DNA, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing, in a medium, a transformant or a transductant including the gene; and collecting the novel fructosyl peptide oxidase from the culture.Type: ApplicationFiled: July 29, 2010Publication date: January 6, 2011Applicant: Kikkoman CorporationInventors: Keiko KUROSAWA, Kozo Hirokawa, Naoki Kajiyama
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Publication number: 20100291623Abstract: The present invention relates to a method for producing ?-glycated dipeptide, which comprises causing protease to act on N-terminal-glycated peptide or N-terminal-glycated protein. The present invention further relates to a method for determining the amount of ?-glycated dipeptide, which comprises causing a fructosyl peptide oxidase to act on the ?-glycated dipeptide obtained by the above method and then determining the amount of the thus generated hydrogen peroxide. According to the present invention, a method for producing ?-glycated dipeptide is provided, which enables the simple, rapid, and efficient production of ?-glycated dipeptide from glycated protein or glycated peptide. Furthermore, according to the present invention, a method for determining the amount of ?-glycated dipeptide is provided, which enables to determine the amount of ?-glycated dipeptide in a highly precise manner within a short time period.Type: ApplicationFiled: May 24, 2010Publication date: November 18, 2010Applicant: Kikkoman CorporationInventors: Kozo HIROKAWA, Keiko Kurosawa, Naoki Kajiyama
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Publication number: 20100255480Abstract: The present invention provides a firefly luciferase for inexpensive, highly accurate and highly sensitive nucleic acid analysis that uses dATP instead of an expensive reagent having low reactivity to DNA polymerase in the manner of dATP?S, a method of analyzing nucleic acid that uses that luciferase, and a kit for analyzing nucleic acid thereof. The present invention relates to a composition for analyzing nucleic acid that contains luciferase for which reactivity to dATP is equal to or less than 1/400 reactivity to ATP, a method of analyzing nucleic acid that comprises the use of that composition, and a kit for analyzing nucleic acid comprising that composition.Type: ApplicationFiled: November 19, 2008Publication date: October 7, 2010Applicant: Kikkoman CorporationInventors: Shigeya Suzuki, Yukako Kodama, Keiko Kurosawa, Eiry Kobatake, Masayasu Mie
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Publication number: 20090011467Abstract: The present invention has an object of providing a novel fructosyl peptide oxidase having superior physicochemical properties such as stability that is useful as an enzyme for clinical diagnosis, and an object of providing a method for producing the fructosyl peptide oxidase. A novel fructosyl peptide oxidase having physicochemical properties useful as an enzyme for clinical diagnosis, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing a microorganism capable of producing the oxidase in a medium; and collecting the oxidase from the culture. Furthermore, a fructosyl peptide oxidase gene coding for a novel fructosyl peptide oxidase, recombinant DNA wherein the gene is inserted into vector DNA, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing, in a medium, a transformant or a transductant including the gene; and collecting the novel fructosyl peptide oxidase from the culture.Type: ApplicationFiled: July 28, 2008Publication date: January 8, 2009Applicant: Kikkoman CorporationInventors: Keiko Kurosawa, Kozo Hirokawa, Naoki Kajiyama
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Patent number: 7419813Abstract: The present invention has an object of providing a novel fructosyl peptide oxidase having superior physicochemical properties such as stability that is useful as an enzyme for clinical diagnosis, and an object of providing a method for producing the fructosyl peptide oxidase. A novel fructosyl peptide oxidase having physicochemical properties useful as an enzyme for clinical diagnosis, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing a microorganism capable of producing the oxidase in a medium; and collecting the oxidase from the culture. Furthermore, a fructosyl peptide oxidase gene coding for a novel fructosyl peptide oxidase, recombinant DNA wherein the gene is inserted into vector DNA, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing, in a medium, a transformant or a transductant including the gene; and collecting the novel fructosyl peptide oxidase from the culture.Type: GrantFiled: March 8, 2005Date of Patent: September 2, 2008Assignee: Kikkoman CorporationInventors: Keiko Kurosawa, Kozo Hirokawa, Naoki Kajiyama
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Publication number: 20070037243Abstract: The present invention relates to a method for producing ?-glycated dipeptide, which comprises causing protease to act on N-terminal-glycated peptide or N-terminal-glycated protein. The present invention further relates to a method for determining the amount of ?-glycated dipeptide, which comprises causing a fructosyl peptide oxidase to act on the ?-glycated dipeptide obtained by the above method and then determining the amount of the thus generated hydrogen peroxide. According to the present invention, a method for producing ?-glycated dipeptide is provided, which enables the simple, rapid, and efficient production of ?-glycated dipeptide from glycated protein or glycated peptide. Furthermore, according to the present invention, a method for determining the amount of ?-glycated dipeptide is provided, which enables to determine the amount of ?-glycated dipeptide in a highly precise manner within a short time period.Type: ApplicationFiled: September 13, 2004Publication date: February 15, 2007Applicant: Kikkoman CorporationInventors: Kozo Hirokawa, Keiko Kurosawa, Naoki Kajiyama
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Patent number: 7101694Abstract: The present invention relates to: an isolated or synthesized gene, which encodes a protein comprising the amino acid sequence represented by SEQ ID NO: 2, an isolated or synthesized gene, which encodes a protein comprising an amino acid sequence comprising a deletion, substitution or addition of one or more amino acids with respect to the amino acid sequence represented by SEQ ID NO: 2 and being capable of regenerating luciferin, an isolated or synthesized gene, which hybridizes with the complementary strand sequence of a DNA comprising the nucleotide sequence represented by SEQ ID NO: 1 under stringent conditions and encodes a protein capable of regenerating luciferin, a recombinant DNA, which is characterized in that the above-described isolated or synthesized gene is inserted into a vector DNA, a transformant or transductant comprising the above-described recombinant DNA, and a process for producing a protein capable of regenerating luciferin, which is characterized in that the method comprises culturing tType: GrantFiled: July 26, 2001Date of Patent: September 5, 2006Assignee: Kikkoman CorporationInventors: Kozo Hirokawa, Keiko Kurosawa, Naoki Kajiyama
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Patent number: 7049113Abstract: The present invention relates to: an isolated or synthesized gene, which encodes a protein comprising the amino acid sequence represented by SEQ ID NO: 2, an isolated or synthesized gene, which encodes a protein comprising an amino acid sequence comprising a deletion, substitution or addition of one or more amino acids with respect to the amino acid sequence represented by SEQ ID NO: 2 and being capable of regenerating luciferin, an isolated or synthesized gene, which hybridizes with the complementary strand sequence of a DNA comprising the nucleotide sequence represented by SEQ ID NO: 1 under stringent conditions and encodes a protein capable of regenerating luciferin, a recombinant DNA, which is characterized in that the above-described isolated or synthesized gene is inserted into a vector DNA, a transformant or transductant comprising the above-described recombinant DNA, and a process for producing a protein capable of regenerating luciferin, which is characterized in that the method comprises culturinType: GrantFiled: July 26, 2001Date of Patent: May 23, 2006Assignee: Kikkoman CorporationInventors: Kozo Hirokawa, Keiko Kurosawa, Naoki Kajiyama
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Patent number: 7018823Abstract: The present invention has an object of providing a novel fructosyl peptide oxidase having superior physicochemical properties such as stability that is useful as an enzyme for clinical diagnosis, and an object of providing a method for producing the fructosyl peptide oxidase. A novel fructosyl peptide oxidase having physicochemical properties useful as an enzyme for clinical diagnosis, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing a microorganism capable of producing the oxidase in a medium; and collecting the oxidase from the culture. Furthermore, a fructosyl peptide oxidase gene coding for a novel fructosyl peptide oxidase, recombinant DNA wherein the gene is inserted into vector DNA, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing, in a medium, a transformant or a transductant including the gene; and collecting the novel fructosyl peptide oxidase from the culture.Type: GrantFiled: September 3, 2002Date of Patent: March 28, 2006Assignee: Kikkoman CorporationInventors: Keiko Kurosawa, Kozo Hirokawa, Naoki Kajiyama