Patents by Inventor Keina YAMAGUCHI
Keina YAMAGUCHI has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230250401Abstract: A method for selecting a cell strain in which reduction of a recombinant protein is suppressed, and uses of the selected cell are disclosed. The method for selecting a cell, includes a first step: measuring the expression level of a gene in a cell, the gene being at least one gene selected from genes comprising any one of the base sequences represented by SEQ ID NOS: 1 to 16 or orthologous genes thereof; and a second step: comparing the expression level of the gene measured in the first step with a control value of the expression level of the gene in a control cell, and evaluating the expression level capable of suppressing reduction of a recombinant protein based on a difference therebetween.Type: ApplicationFiled: April 14, 2023Publication date: August 10, 2023Applicant: KYOWA KIRIN CO., LTD.Inventors: Koichi YAMAMOTO, Yuichi MATSUMOTO, Keina YAMAGUCHI, Hiroto FUJII, Toshiyuki SUZAWA
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Publication number: 20190316096Abstract: An object of the present invention is to provide a method for selecting a cell strain in which reduction of a recombinant protein is suppressed. The present invention relates to a method for selecting a cell, comprising a first step: measuring the expression level of a gene in a cell, the gene being at least one gene selected from genes comprising any one of the base sequences represented by SEQ ID NOS: 1 to 16 or orthologous genes thereof; and a second step: comparing the expression level of the gene measured in the first step with a control value of the expression level of the gene in a control cell, and evaluating the expression level capable of suppressing reduction of a recombinant protein based on a difference therebetween.Type: ApplicationFiled: December 27, 2017Publication date: October 17, 2019Applicant: KYOWA HAKKO KIRIN COInventors: Koichi YAMAMOTO, Yuichi MATSUMOTO, Keina YAMAGUCHI, Hiroto FUJII, Toshiyuki SUZAWA
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Patent number: 10358671Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.Type: GrantFiled: June 30, 2017Date of Patent: July 23, 2019Assignees: KYOWA HAKKO KIRIN CO., LTD, Inter-University Research Institute Corporation Research Organization of Information and SystemsInventors: Koichi Kawakami, Keina Yamaguchi, Risa Ogawa, Masayoshi Tsukahara
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Publication number: 20170306378Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.Type: ApplicationFiled: June 30, 2017Publication date: October 26, 2017Applicants: KYOWA HAKKO KIRIN CO., LTD, Inter-University Research Institute Corporation Re search Organization of Information and SystemsInventors: Koichi KAWAKAMI, Keina YAMAGUCHI, Risa OGAWA, Masayoshi TSUKAHARA
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Patent number: 9725750Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.Type: GrantFiled: April 17, 2015Date of Patent: August 8, 2017Assignees: KYOWA HAKKO KIRIN CO., LTD, Inter-University Research Institute Corporation Research Organization of Information and SystemsInventors: Koichi Kawakami, Keina Yamaguchi, Risa Ogawa, Masayoshi Tsukahara
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Patent number: 9102971Abstract: The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; and a method for producing the protein of interest comprising suspension-culturing a suspension mammalian cell which produces the protein of interest; and an a suspension mammalian cell which expresses the protein of interest.Type: GrantFiled: December 15, 2011Date of Patent: August 11, 2015Assignees: Inter-University Research Institute Corporation Research Organization of Information and Systems, KYOWA HAKKO KIRIN CO., LTDInventors: Megumi Kurokawa, Keina Yamaguchi, Risa Ogawa, Masayoshi Tsukahara, Koichi Kawakami, Yoko Hayashi
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Publication number: 20150218610Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.Type: ApplicationFiled: April 17, 2015Publication date: August 6, 2015Applicants: KYOWA HAKKO KIRIN CO., LTD, Inter-University Research-Institute Corporation Research Organization of Information & SystemsInventors: Koichi KAWAKAMI, Keina YAMAGUCHI, Risa OGAWA, Masayoshi TSUKAHARA
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Patent number: 9034649Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.Type: GrantFiled: June 11, 2010Date of Patent: May 19, 2015Assignees: Inter-University Research Institute Corporation Research Organization of Information and Systems, KYOWA HAKKO KIRIN CO., LTDInventors: Koichi Kawakami, Keina Yamaguchi, Risa Ogawa, Masayoshi Tsukahara
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Publication number: 20120196327Abstract: The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; and a method for producing the protein of interest comprising suspension-culturing a suspension mammalian cell which produces the protein of interest; and an a suspension mammalian cell which expresses the protein of interest.Type: ApplicationFiled: December 15, 2011Publication date: August 2, 2012Applicants: Kyowa Hakko Kirin Co., Ltd., Inter-University Research Institute Corporation Research Organization of Information and SystemsInventors: Megumi KUROKAWA, Keina YAMAGUCHI, Risa OGAWA, Masayoshi TSUKAHARA, Koichi KAWAKAMI, Yoko HAYASHI
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Publication number: 20110045532Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.Type: ApplicationFiled: June 11, 2010Publication date: February 24, 2011Applicants: Inter-University Research Institute Corporation, Research Organization of Information and systems, KYOWA HAKKO KIRIN CO., LTD.Inventors: Koichi KAWAKAMI, Keina YAMAGUCHI, Risa OGAWA, Masayoshi TSUKAHARA