Abstract: In a nucleic acid amplification chip, a filter is provided inside or outside a PCR reaction container with respect to a micro-flow channel. Here, the filter is fixed to the PCR reaction container via a fixing portion. In this case, since the fixing portion is formed, the reliability of fixing the filter to the PCR reaction container can be improved. For this reason, the leakage of an evaporated sample solution (aerosol) from a gap between the filter and the PCR reaction container can be suppressed. Further, since the fixing portion is formed, the leakage of the evaporated sample solution (aerosol) can be suppressed without increasing the thickness of the filter, so that the filter can be made thin. As a result, the overall thickness of the nucleic acid amplification chip can also be reduced, so that the degree of freedom in designing the nucleic acid amplification chip is improved.

Abstract: A nucleic acid amplification device, on which a nucleic acid amplification chip is placed, includes: temperature regulation units forming a denaturation temperature zone and an extension and annealing temperature zone; liquid delivery mechanisms that equalize air pressures of an air suction portion and an air discharge portion when liquid delivery is stopped; a detection unit that detects a sample solution in the nucleic acid amplification chip; and a control unit that controls the liquid delivery mechanisms based on a detection result of the detection unit. The detection unit includes an infrared light source that emits an infrared ray, and an infrared detection element that detects the infrared ray.