Abstract: A detection method includes: preparing sample image data of a sample in which a target substance is labeled with a labeling substance; acquiring first background image data in which an image feature amount of a single bright spot in the sample image data is reduced by a first filter; acquiring second background image data in which an image feature amount of a dense bright spot in the sample image data is reduced by a second filter; synthesizing the first background image data and the second background image data on a basis of brightness information of the sample image data and acquiring synthesized background image data; and obtaining difference image data that is a difference between the sample image data and the synthesized background image data.

Abstract: Embodiments of a coupling system to couple a rotating clutch portion with a shaft include a sleeve and at least one key. The sleeve is positioned between the rotating clutch portion and the shaft. The sleeve has at least one key slot to receive the at least one key. The rotating clutch portion includes a key slot to receive one of the at least one keys. The shaft includes a key slot to receive one of the at least one keys. When the at least one keys is received by the various key slots, the rotating clutch portion is rotationally coupled to the shaft.

Abstract: Cell cultures for treating peripheral arterial disease, methods for producing the cell cultures and methods for treating peripheral arterial disease using the cell cultures are provided. The cell cultures may be 100 to 500 ?m in size and may possess an extracellular matrix on an outer surface of the cell culture, and the like are provided.

Abstract: Provided is an artificial lawn that has a high durability and does not undergo fibrillation over a long time. In the artificial lawn, flat filament yarns, which are produced by uniaxially stretching a crystalline plastic material to give a crystal orientation degree of 0.75-0.90, are used as piles (3) to be implanted in a base fabric (2).