Abstract: The present disclosure relates to compositions and methods for reducing the concentration of extendable free and buried primers relative to amplification product in a sample. The disclosed methods and compositions can be used to reduce or eliminate index hopping in a next generation sequencing (NGS) platform.

Abstract: A module for constructing a modular bridge suitable for use in both a dry span and a floating role comprising a buoyant structure having interconnection apparatus for connecting the module end to end to an adjacent similar module to form a bridge and having one or more link members which are releasably securable to the underside of the bridge module by link securing apparatus. Each link is provided at one end with a double limb connection member and at the other with a complementary single limb connection member, both provided with holes for insertion of a pin to connect two links from adjacent bridge modules together. The releasably securable link members are removed from a bridge assembled for a floating role to reduce redundant weight.

Abstract: Apparatus for continuous extrusion of metals in which feed is introduced into a circumferential groove 26 (FIG. 3) in a rotating wheel 2 to contact arcuate tooling 22 and an abutment 24. The abutment 4 is spaced from the walls of the groove 26 so that in operation a lining of the feed material is produced. Indentations 52 assist in holding the lining in place. The extrusion is generally in a radial direction through an aperture 58. The extrusion orifices (not shown) may be of part annular cross-section of different area. The extrusion aperture may extend from outside the groove 26 to permit of increased orifice area.

Abstract: A means for use in providing a connection between a cable and a movable body, more especially for R.A.S. systems, comprises a latching unit having means for attachment to said body and a recess associated with a latch, the arrangement also comprising a terminating member having means for attachment to the cable and being capable of introduction into the recess for engagement by the latch, the latter being associated with locking means for securing it in an operating position in which withdrawal of the terminating member is prevented, and release means for actuating the locking means to permit the latch to move to a release position so as to allow the terminating member to be withdrawn. Means may be provided for preventing inadvertent operation of the latch and accidental release of the member.

Abstract: A spooler arranged for leading wire to a rotating spool through a wire tension controller and winding it onto the spool while distributing it in alternating directions along the spool between flanges thereof has means for varying the length of the distribution in either direction, arranged to be so controlled by means responsive to changes in the length of the wire in the wire tension controller in a period adjacent the moment of change to the other distribution direction as at least to reduce wound wire profile deficits or excesses adjacent the spool flanges.