Patents by Inventor Keith R. Roesler
Keith R. Roesler has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11913035Abstract: Provided are compositions comprising polynucleotides encoding modified diacylglycerol acyltransferase-1 (DGAT1) polypeptides having improved properties, such as increased enzymatic activity and/or increased stability. Plants, plant cells, seed, grain and comprising the polynucleotides are provided which have one or more of increased fatty acid or protein content. Methods of generating the polynucleotides in plant cells include transformation and genetic modification. Methods of employing the polynucleotides in plants, methods for increasing DGAT1 activity in a plant, and methods for increasing fatty acid content or protein content in a plant are provided.Type: GrantFiled: September 19, 2022Date of Patent: February 27, 2024Assignee: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Kayla S Flyckt, Kristin Haug Collet, Zhan-Bin Liu, Keith R Roesler, Bo Shen, Laura L Wayne
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Publication number: 20230151338Abstract: Provided are compositions comprising polynucleotides encoding modified diacylglycerol acyltransferase-1 (DGAT1) polypeptides having improved properties, such as increased enzymatic activity and/or increased stability. Plants, plant cells, seed, grain and comprising the polynucleotides are provided which have one or more of increased fatty acid or protein content. Methods of generating the polynucleotides in plant cells include transformation and genetic modification. Methods of employing the polynucleotides in plants, methods for increasing DGAT1 activity in a plant, and methods for increasing fatty acid content or protein content in a plant are provided.Type: ApplicationFiled: September 19, 2022Publication date: May 18, 2023Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: KAYLA S FLYCKT, KRISTIN HAUG COLLET, ZHAN-BIN LIU, KEITH R ROESLER, BO SHEN, LAURA L WAYNE
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Patent number: 11479759Abstract: Provided are compositions comprising polynucleotides encoding modified diacylglycerol acyltransferase-1 (DGAT1) polypeptides having improved properties, such as increased enzymatic activity and/or increased stability. Plants, plant cells, seed, grain and comprising the polynucleotides are provided which have one or more of increased fatty acid or protein content. Methods of generating the polynucleotides in plant cells include transformation and genetic modification. Methods of employing the polynucleotides in plants, methods for increasing DGAT1 activity in a plant, and methods for increasing fatty acid content or protein content in a plant are provided.Type: GrantFiled: May 30, 2019Date of Patent: October 25, 2022Inventors: Kayla S Flyckt, Kristin Haug Collet, Zhan-Bin Liu, Keith R Roesler, Bo Shen, Laura L Wayne
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Publication number: 20210207103Abstract: Provided are compositions comprising polynucleotides encoding modified diacylglycerol acyltransferase-1 (DGAT1) polypeptides having improved properties, such as increased enzymatic activity and/or increased stability. Plants, plant cells, seed, grain and comprising the polynucleotides are provided which have one or more of increased fatty acid or protein content. Methods of generating the polynucleotides in plant cells include transformation and genetic modification. Methods of employing the polynucleotides in plants, methods for increasing DGAT1 activity in a plant, and methods for increasing fatty acid content or protein content in a plant are provided.Type: ApplicationFiled: May 30, 2019Publication date: July 8, 2021Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: KAYLA S FLYCKT, KRISTIN HAUG COLLET, ZHAN-BIN LIU, KEITH R ROESLER, BO SHEN, LAURA L WAYNE
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Publication number: 20150259696Abstract: Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences encompassing a guard-cell-preferred promoter which drives preferential expression of gene products in guard cells. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.Type: ApplicationFiled: October 10, 2013Publication date: September 17, 2015Inventors: Shane Abbitt, Mark Alan Chamberlin, Keith R. Roesler
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Patent number: 7211431Abstract: The invention provides expression cassettes comprising isolated nucleic acids from modified Hordeum vulgare CI-2 polypeptides that are involved in enhancing the essential amino acid content of a plant.Type: GrantFiled: August 16, 2004Date of Patent: May 1, 2007Assignee: Pioneer Hi-Bred International, Inc.Inventors: Aragula Gururaj Rao, Keith R. Roesler
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Patent number: 7160988Abstract: We disclose isolated terminal fusion polypeptides comprising a soybean glycinin or proglycinin polypeptide operably linked to the peptide of SEQ ID NO:12.Type: GrantFiled: March 5, 2004Date of Patent: January 9, 2007Assignee: Pioneer Hi-Bred International, Inc.Inventors: Keith R. Roesler, Jennifer K. Barry, Aragula Gururaj Rao
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Patent number: 6800726Abstract: The invention provides isolated nucleic acids and their encoded polypeptides that are involved in enhancing the essential amino acid content of a plant. The polypeptide may be derived from a protease inhibitor, and more specifically, a chymotrypsin inhibitor. Chymotrypsin inhibitors that may be modified for use in the invention are present in many plant species. Barley (Hordeum vulgare) was initially used to obtain the chymotrypsin inhibitor modified for use in the present invention. Other plant species that may be used as a source for chymotrypsin inhibitor for use in the present invention include Zea Mays, Vicia faba, Cucurbita maxima, Canavalia lineata, Vigna angularis, Nicotiana tabacum, Nicotiana sylvestris, Sambucus nigra, Momordica charantia, Solanum tuberosum, Lycopersicon peruvianum, Lycopersicon esculentum, Amaranthus caudatus and Arabidopsis thalania. Optionally there is also a decrease in protease inhibitory activity of the polypeptide.Type: GrantFiled: May 13, 1999Date of Patent: October 5, 2004Assignee: Pioneer Hi-Bred International, Inc.Inventors: Aragula Gururaj Rao, Keith R. Roesler
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Patent number: 5962767Abstract: An Arabidopsis acetyl-CoA carboxylase (ACCase) gene has been isolated and sequenced. The 10 kb gene encodes a 251 kD cytosolic ACCase isozyme. The nucleic acid sequence of the gene is set forth in SEQ ID No. 1 and has been accorded GenBank Accession No. L27074. The cDNA sequence and deduced amino acid sequence of the cDNA are set forth in SEQ ID Nos. 2 and 3, respectively. By controlling expression of the gene of the present invention, carboxylation of acetyl-CoA to produce malonyl-CoA may be controlled. Thus, by introducing constructs of the gene of the present invention in sense or anti-sense orientation, carboxylation of acetyl-CoA to produce malonyl-CoA may be increased or decreased. Consequently, fatty acid synthesis and elongation in plants and seeds which depends on malonyl-CoA may also be controlled.Type: GrantFiled: January 29, 1997Date of Patent: October 5, 1999Assignee: Board of Trustees Operating Michigan State UniversityInventors: John B. Ohlrogge, Keith R. Roesler, Basil S. Shorrosh
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Patent number: 5925805Abstract: An Arabidopsis acetyl-CoA carboxylase (ACCase) gene has been isolated and sequenced. The 10-kb gene encodes a 251-kd cytosolic ACCase isozyme. The nucleic acid sequence of the gene is set forth in SEQ ID No. 1 and has been accorded GenBank Accession No. L27074. The cDNA sequence and deduced amino acid sequence of the cDNA are set forth in SEQ ID Nos. 2 and 3, respectively. By controlling expression of the gene of the present invention, carboxylation of acetyl-CoA to produce malonyl-CoA may be controlled. Thus, by introducing constructs of the gene of the present invention in sense or anti-sense orientation, carboxylation of acetyl-CoA to produce malonyl-CoA may be increased or decreased. Consequently, fatty acid synthesis and elongation in plants and seeds which depends on malonyl-CoA may also be controlled.Type: GrantFiled: July 1, 1996Date of Patent: July 20, 1999Assignee: Board of Trustees Operating Michigan State UniversityInventors: John B. Ohlrogge, Keith R. Roesler, Basil S. Shorrosh