Abstract: Methods of expanding and selecting disease associated T-cells, continuous T-cell lines as well as T-cell lines obtainable by these methods are disclosed. Furthermore, pharmaceutical compositions and vaccines comprising activated disease associated T-cell are disclosed. The uses of the T-cell and T-cell lines are numerous and include methods of diagnosis, methods for the treatment, alleviation or prevention of diseases associated with activation of T-cells, methods of testing the effect of medicaments against T-cell associated diseases, methods of detecting T-cell growth factors, methods of monitoring the response to treatment, alleviation or prevention of diseases associated with activation of T-cells, and methods of identifying disease associated antigens.

Abstract: The invention describes continuously growing, normal T-lymphocyte cell lines (T-lymphocyte lines) comprising nucleic acids sequences encoding immune receptors, for example T-cell receptors, with defined antigen specificity, wherein the nucleotide sequences are operably linked to an expression signal not natively associated therewith. Further, this invention is directed to methods of adoptive immunotherapy. In particular methods for allogeneic adoptive immunotherapy are provided. This invention further relates to methods for adoptive immunotherapy in the treatment of cancer in a human being, including therapy of malignant melanoma. Moreover this invention relates to methods for cultivating unlimited amounts of activated T-lymphocytes stably expressing a T-cell receptor with defined tumour antigen specificity and reactivity.

Abstract: Methods of expanding and selecting disease associated T-cells, continuous T-cell lines as well as T-cell lines obtainable by these methods are disclosed. Furthermore, pharmaceutical compositions and vaccines comprising activated disease associated T-cell are disclosed. The uses of the T-cells and T-cell lines are numerous and include methods of diagnosis, methods for the treatment, alleviation or prevention of diseases associated with activation of T-cells, methods of testing the effect of medicaments against T-cell associated diseases, methods of detecting T-cell growth factors, methods of monitoring the response to treatment, alleviation or prevention of diseases associated with activation of T-cells, and methods of identifying disease associated antigens.