Abstract: Provided herein are IL-2 muteins, IL-2 mutein Fc-fusion molecules, anti-IL-2 antibodies, and complexes comprising an anti IL-2 antibody bound to an IL-2 cytokine that preferentially expand and activate T regulatory cells and are amenable to large scale production. Also provided herein are variant human IgG1 Fc molecules lacking or with highly reduced effector function and high stability despite lacking glycosylation at N297. Also provided herein are linker peptides that are glycosylated when expressed in mammalian cells. Also provided herein are methods of making and using the compositions of the present invention.

Abstract: The present invention provides, in various embodiments, genetically modified aerobic bacteria, polynucleotides and methods for expressing and/or harvesting electrically conductive protein nanowires (e-PNs). The present invention also provides e-PNs produced using the genetically modified aerobic bacteria, polynucleotides and methods.

Abstract: Improved electrically conductive pili were generated from a natural pilus protein from the microorganism Geobacter sulfurreducens. Substituting a tryptophan for the phenylalanine at position F51 and a tryptophan for the tyrosine at position Y57 of the pilus monomer substantially increased the conductivity of the pili and reduced their diameter to 1.5 nm. Substantial improvements in conductivity were also achieved by substituting an additional tyrosine, histidine, and phenylalanine in the pilus monomer to mimic the monomer of Geobacter metallireducens, but the pili retained the typical Geobacter sulfurreducens wild-type diameter of 3 nm.

Abstract: Improved electrically conductive pili were generated from a natural pilus protein from the microorganism Geobacter sulfurreducens. Substituting a tryptophan for the phenylalanine at position F51 and a tryptophan for the tyrosine at position Y57 of the pilus monomer substantially increased the conductivity of the pili and reduced their diameter to 1.5 nm. Substantial improvements in conductivity were also achieved by substituting an additional tyrosine, histidine, and phenylalanine in the pilus monomer to mimic the monomer of Geobacter metallireducens, but the pili retained the typical Geobacter sulfurreducens wild-type diameter of 3 nm.

Abstract: A sensor that measures microbial activity as a surrogate value for the biologically active content of soil, aquatic sediments, or groundwater. An anode, such as a graphite anode that can support a biofilm, is connected by way of a resistor to a cathode. The anode is in contact with either soil, sediment, or immersed in the groundwater of a subsurface monitoring well. The biofilm generates electrons as a consequence of chemical interactions with materials such as acetate dissolved in the soil or sediment waters or groundwater. The cathode is located in soil or water adjacent to the ground, which can be aerobic, so that a reaction that consumes electrons occurs at the cathode. The current flowing through the resistor is a measure of the biological activity at the anode, which correlates with the flux of fuel such as acetate to the anode.

Abstract: The invention provides systems and methods for generating organic compounds using carbon dioxide as a source of carbon and electrical current as an energy source. In one embodiment, a reaction cell is provided having a cathode electrode and an anode electrode that are connected to a source of electrical power, and which are separated by a permeable membrane. A biological film is provided on the cathode. The biological film comprises a bacterium that can accept electrons and that can convert carbon dioxide to a carbon-bearing compound and water in a cathode half-reaction. At the anode, water is decomposed to free molecular oxygen and solvated protons in an anode half-reaction. The half-reactions are driven by the application of electrical current from an external source. Compounds that have been produced include acetate, butanol, 2-oxobutyrate, propanol, ethanol, and formate.

Abstract: An aerobic microbial fuel cell anode electrode, a fuel cell using the anode, and methods of use. An anode electrode having a conductive exterior surface and having sufficient porosity to allow a fuel-bearing liquid flowing in a cavity within the anode to escape and to supply fuel to a biologically active microbe film grown on the exterior of the anode is situated in the fuel cell. When operated in an aerobic environment, such as water, the anode and a cathode can supply electrical power to a load without the need for a semi-permeable membrane between the anode and the cathode. Several embodiments in which the anode electrode is machined from a graphite block or cylinder are described. Conditions for growing the biologically active film and for operating the fuel cell are described.

Abstract: The invention provides systems and methods for generating organic compounds using carbon dioxide as a source of carbon and electrical current as an energy source. In one embodiment, a reaction cell is provided having a cathode electrode and an anode electrode that are connected to a source of electrical power, and which are separated by a permeable membrane. A biological film is provided on the cathode. The biological film comprises a bacterium that can accept electrons and that can convert carbon dioxide to a carbon-bearing compound and water in a cathode half-reaction. At the anode, water is decomposed to free molecular oxygen and solvated protons in an anode half-reaction. The half-reactions are driven by the application of electrical current from an external source. Compounds that have been produced include acetate, butanol, 2-oxobutyrate, proponal, ethanol, and formate.

Abstract: An aerobic microbial fuel cell anode electrode, a fuel cell using the anode, and methods of use. An anode electrode having a conductive exterior surface and having sufficient porosity to allow a fuel-bearing liquid flowing in a cavity within the anode to escape and to supply fuel to a biologically active microbe film grown on the exterior of the anode is situated in the fuel cell. When operated in an aerobic environment, such as water, the anode and a cathode can supply electrical power to a load without the need for a semi-permeable membrane between the anode and the cathode. Several embodiments in which the anode electrode is machined from a graphite block or cylinder are described. Conditions for growing the biologically active film and for operating the fuel cell are described.