Patents by Inventor Keming Song
Keming Song has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20130059362Abstract: The present invention provides compositions and methods for monitoring cellular signal transduction events. In particular, signal transduction events may be monitored by the use of biosensors comprising protein-binding domains, which bind signal-transduction proteins, and fluorescent proteins. Provided herein, therefore, methods for detecting activation of signal transduction proteins or screening for agents that modulate the activity of signal transduction proteins. Also provided are cells comprising the biosensors; lentiviral particles comprising biosensor coding sequence; and kits comprising the lentiviral particles.Type: ApplicationFiled: April 13, 2011Publication date: March 7, 2013Applicant: SIGMA-ALDRICH CO., LLCInventors: John Fetter, Dmitry Malkov, Nathan Zenser, Keming Song
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Patent number: 7504238Abstract: Amplification mixtures, kits, amplicons, kits and processes are provided for amplifying a nucleic acid. In particular, provided are processes which utilize an amplification mixture comprising a polymerase, a deoxynucleotidetriphosphate (dNTP) mixture which contains modified dNTPs, a first primer and a second primer. Further provided are dNTP mixtures which contain modified dNTPs for at least two of the four nucleotide triphosphates, which when incorporated into a polynucelotide, impart resistance to enzymatic degradation by an exonuclease at the sites of incorporation of the modified dNTPs. Also provided are the amplicons and vectors which incorporate the modified nucleotides.Type: GrantFiled: May 2, 2005Date of Patent: March 17, 2009Assignee: Sigma-Aldrich Co.Inventors: Brian Ward, Chuan Li, Keming Song, Kristen E. Bettinger, Stephanie Uder
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Publication number: 20080039616Abstract: The present invention generally relates to polypeptide tags and the purification of polypeptides, proteins, and protein fragments displaying such tags. More particularly, the invention is directed to novel polypeptides, nucleic acid sequences and vectors encoding novel polypeptides containing antigenic determinants. Also provided are methods and kits for using such polypeptides for the purification of target peptides, as well as methods of constructing vectors encoding the novel polypeptides and the novel polypeptides linked to a target peptide.Type: ApplicationFiled: September 29, 2006Publication date: February 14, 2008Applicant: Sigma-Aldrich Co.Inventors: Nathan Zenser, Kristin Bettinger, Keming Song
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Publication number: 20070264638Abstract: This invention provides methods, compounds, and kits for identifying ginseng (Panax) species and for distinguishing between ginseng and imposter species based upon molecular variations. In particular, amplification primers are provided that amplify polymorphic regions of nucleic acid sequences to generate distinct amplification profiles in the different plant species. A plant may be identified by comparing its amplification profile to known amplification profiles of Panax and imposter species.Type: ApplicationFiled: May 10, 2006Publication date: November 15, 2007Inventors: Fan Zhang, Keming Song, Nathan Zenser
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Publication number: 20070264639Abstract: This invention provides methods, compounds, and kits for identifying Echinacea species and for distinguishing between Echinacea and imposter species based upon molecular variations. In particular, amplification primers are provided that amplify specific regions of nucleic acid sequences to generate unique amplification profiles in the different plant species. A plant may be identified by comparing its amplification profile to known amplification profiles of Echinacea and imposter species.Type: ApplicationFiled: May 10, 2006Publication date: November 15, 2007Inventors: Nathan Zenser, Keming Song, Fan Zhang
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Publication number: 20060003353Abstract: Amplification mixtures, kits, amplicons, kits and processes are provided for amplifying a nucleic acid. In particular, provided are processes which utilize an amplification mixture comprising a polymerase, a deoxynucleotidetriphosphate (dNTP) mixture which contains modified dNTPs, a first primer and a second primer. Further provided are dNTP mixtures which contain modified dNTPs for at least two of the four nucleotide triphosphates, which when incorporated into a polynucelotide, impart resistance to enzymatic degradation by an exonuclease at the sites of incorporation of the modified dNTPs. Also provided are the amplicons and vectors which incorporate the modified nucleotides.Type: ApplicationFiled: May 2, 2005Publication date: January 5, 2006Applicant: Sigma-Aldrich Co.Inventors: Brian Ward, Leanne Snyder, Chuan Li, Keming Song, Kristen Opper, Stephanie Uder, Ron Hernan
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Publication number: 20050208564Abstract: Amplification mixtures, kits, amplicons, kits and processes are provided for amplifying a nucleic acid. In particular, provided are processes which utilize an amplification mixture comprising a polymerase, a deoxynucleotidetriphosphate (DNTP) mixture which contains modified dNTPs, a first primer and a second primer. Further provided are DNTP mixtures which contain modified dNTPs for at least two of the four nucleotide triphosphates, which when incorporated into a polynucelotide, impart resistance to enzymatic degradation by an exonuclease at the sites of incorporation of the modified dNTPs. Also provided are the amplicons and vectors which incorporate the modified nucleotides.Type: ApplicationFiled: May 2, 2005Publication date: September 22, 2005Applicant: Sigma-Aldrich Co.Inventors: Brian Ward, Leanne Snyder, Chuan Li, Keming Song, Kristen Opper, Stephanie Uder, Ron Hernan
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Patent number: 6902914Abstract: Amplification mixtures, kits, amplicons, kits and processes are provided for amplifying a nucleic acid. In particular, provided are processes which utilize an amplification mixture comprising a polymerase, a deoxynucleotidetriphosphate (dNTP) mixture which contains modified dNTPs, a first primer and a second primer. Further provided are dNTP mixtures which contain modified dNTPs for at least two of the four nucleotide triphosphates, which when incorporated into a polynucelotide, impart resistance to enzymatic degradation by an exonuclease at the sites of incorporation of the modified dNTPs. Also provided are the amplicons and vectors which incorporate the modified nucleotides.Type: GrantFiled: November 15, 2001Date of Patent: June 7, 2005Assignee: Sigma-Aldrich, Co.Inventors: Brian Ward, Leanne M. Snyder, Chuan Li, Keming Song, Kristen E. Opper, Stephanie Uder, Ron Hernan
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Publication number: 20050003350Abstract: Methods are provided for amplifying regions of the HIV pol gene amplifying double-stranded nucleic acid template derived from HIV tube RT-PCR with novel PCR primers to produce amplified target sequences. Methods are also provided for analyzing the nucleotide sequence of these amplified targets using novel sequencing primers and the data is analyzed. The determined nucleotide sequence can be compared to the sequence of known drug resistance mutations in the HIV pot gene to determine the viral genotype.Type: ApplicationFiled: August 10, 2004Publication date: January 6, 2005Inventors: Leslie Johnston-Dow, Lisa Demeter, Camille White, Keming Song, Robert Kohlenberger, Morgan Conrad, Angela Myers
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Patent number: 6806046Abstract: Methods are provided for amplifying regions of the HIV pol gene amplifying double-stranded nucleic acid template derived from HIV tube RT-PCR with novel PCR primers to produce amplified target sequences. Methods are also provided for analyzing the nucleotide sequence of these amplified targets using novel sequencing primers and the data is analyzed. The determined nucleotide sequence can be compared to the sequence of known drug resistance mutations in the HIV pol gene to determine the viral genotype.Type: GrantFiled: December 31, 2002Date of Patent: October 19, 2004Assignee: Applera CorporationInventors: Leslie A. Johnston-Dow, Lisa Demeter, Camille B. White, Keming Song, Robert Kohlenberger, Morgan Conrad, Angela Myers
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Publication number: 20030219725Abstract: Methods are provided for amplifying regions of the HIV pol gene amplifying double-stranded nucleic acid template derived from HIV tube RT-PCR with novel PCR primers to produce amplified target sequences. Methods are also provided for analyzing the nucleotide sequence of these amplified targets using novel sequencing primers and the data is analyzed. The determined nucleotide sequence can be compared to the sequence of known drug resistance mutations in the HIV pol gene to determine the viral genotype.Type: ApplicationFiled: December 31, 2002Publication date: November 27, 2003Inventors: Leslie A. Johnston-Dow, Lisa Demeter, Camille B. White, Keming Song, Robert Kohlenberger, Morgan Conrad, Angela Myers
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Patent number: 6623919Abstract: Disclosed herein are methods and kits for the simultaneous detection of hepatitis C virus and human immunodeficiency virus in biological samples from human subjects.Type: GrantFiled: January 28, 2000Date of Patent: September 23, 2003Assignee: Ortho-Clinical Diagnostics, INCInventors: Kevin M. Gorman, David R. Patterson, Jeffrey M. Linnen, Keming Song
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Publication number: 20030099956Abstract: Amplification mixtures, kits, amplicons, kits and processes are provided for amplifying a nucleic acid. In particular, provided are processes which utilize an amplification mixture comprising a polymerase, a deoxynucleotidetriphosphate (dNTP) mixture which contains modified dNTPs, a first primer and a second primer. Further provided are dNTP mixtures which contain modified dNTPs for at least two of the four nucleotide triphosphates, which when incorporated into a polynucelotide, impart resistance to enzymatic degradation by an exonuclease at the sites of incorporation of the modified dNTPs. Also provided are the amplicons and vectors which incorporate the modified nucleotides.Type: ApplicationFiled: November 15, 2001Publication date: May 29, 2003Inventors: Brian Ward, Leanne M. Snyder, Chuan Li, Keming Song, Kristen E. Opper, Stephanie Uder, Ron Hernan
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Patent number: 6531588Abstract: Methods are provided for amplifying regions of the HIV pol gene amplifying double-stranded nucleic acid template derived from HIV tube RT-PCR with novel PCR primers to produce amplified target sequences. Methods are also provided for analyzing the nucleotide sequence of these amplified targets using novel sequencing primers and the data is analyzed. The determined nucleotide sequence can be compared to the sequence of known drug resistance mutations in the HIV pol gene to determine the viral genotype.Type: GrantFiled: March 5, 2002Date of Patent: March 11, 2003Assignee: Applera CorporationInventors: Leslie A. Johnston-Dow, Lisa Demeter, Camille B. White, Keming Song, Robert Kohlenberger, Morgan Conrad, Angela Myers
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Patent number: 6379957Abstract: Methods are provided for amplifying regions of the HIV pol gene amplifying double-stranded nucleic acid template derived from HIV tube RT-PCR with novel PCR primers to produce amplified target sequences. Methods are also provided for analyzing the nucleotide sequence of these amplified targets using novel sequencing primers and the data is analyzed. The determined nucleotide sequence can be compared to the sequence of known drug resistance mutations in the HIV pol gene to determine the viral genotype.Type: GrantFiled: September 21, 1998Date of Patent: April 30, 2002Inventors: Leslie A. Johnston-Dow, Lisa Demeter, Camille B. White, Keming Song, Robert Kohlenberger, Morgan Conrad, Angela Myers
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Patent number: 6303293Abstract: Disclosed herein are methods and kits for the detection of human immunodeficiency virus in biological samples from human subjects. Oligonucleotide reverse transcription primers for use in such methods and kits for detection of human immunodeficiency virus are also described.Type: GrantFiled: January 28, 2000Date of Patent: October 16, 2001Assignee: Ortho-Clinical Diagnostics, Inc.Inventors: David R. Patterson, John A. Puskas, Keming Song, Jeffrey M. Linnen