Abstract: A method of selectively separating a phospholipid from a sample solution containing the phospholipid is provided. The method comprises: adsorbing calcium ions to a filler, at least a surface of the filler being constituted of a calcium phosphate-based compound; supplying the sample solution into an apparatus having a filling space, wherein the filling space being filled with the filler so that the phospholipid contained in the sample solution is adsorbed to the filler through the calcium ions; supplying an organic solvent-based eluate into the filling space of the apparatus to obtain a liquid containing the phospholipid and discharged from the apparatus; and fractionating the obtained liquid per a predetermined amount to thereby separate the phospholipid from the sample solution.

Abstract: A method of selectively separating a phospholipid from a sample solution containing the phospholipid is provided. The method comprises: adsorbing calcium ions to a filler, at least a surface of the filler being constituted of a calcium phosphate-based compound; supplying the sample solution into an apparatus having a filling space, wherein the filling space being filled with the filler so that the phospholipid contained in the sample solution is adsorbed to the filler through the calcium ions; supplying an organic solvent-based eluate into the filling space of the apparatus to obtain a liquid containing the phospholipid and discharged from the apparatus; and fractionating the obtained liquid per a predetermined amount to thereby separate the phospholipid from the sample solution.

Abstract: A method of separating a fluorescent protein from a sample containing a plurality of proteins containing the fluorescent protein is provided.

Abstract: Carrier and method of its manufacture having immobilized antigens or antibodies, including a carrier having a surface, in which at least the surface of the carrier is formed of a calcium phosphate based compound; antiligands provided on and surrounding the surface of the carrier; a blocking layer formed of a protein having low interaction with antigens or antibodies, the blocking layer being formed on a portion of the surface of the carrier where the antiligands are not provided; and antigens or antibodies each having a portion that is irrelevant to the antigen-antibody reaction on which a ligand is provided, the antigens or antibodies being immobilized to the surface of the carrier through the bonding between the ligands and the antiligands with the blocking layer effectively preventing antigens or antibodies from being directly absorbed to the surface without bonding between the ligands and the antiligands.

Abstract: A carrier for cell culture which enables cells to efficiently grow thereon is provided. A carrier comprises a particulate base body mainly formed of a resin material and a coating layer. The coating layer is formed from particles of a calcium phosphate-based compound, and the particles are partially embedded in the base body at the vicinity of the surface thereof whereby the coating layer coats the surface of the base body with a calcium phosphate-based compound. Such a carrier enables cells to adhere to and grow on the surface thereof. Therefore, the carrier can be used for cell culture, especially high-density three-dimensional cell culture. The coating layer can be formed, for example, by colliding porous particles of a calcium phosphate-based compound against the surface of the base body.

Abstract: A collagen-coated carrier that has excellent cell adhesion properties and that allows excellent cell growth thereon is provided. Further, a method for manufacturing such a collagen-coated carrier efficiently and reliably is also provided. The collagen-coated carrier includes a base material (carrier) composed of a calcium phosphate-based compound and a coating layer provided so as to cover the surface of the base material. The coating layer is composed of collagen and a protein having a high affinity for the collagen. Such a coating layer covering the surface of the base material is formed by allowing the base material to firmly adsorb the collagen via the protein. The protein preferably has a collagen receptor. More preferably, the protein contains at least one of fibronectin and integrin as a main ingredient.

Abstract: Carrier and method of its manufacture having immobilized antigens or antibodies, including a carrier having a surface, in which at least the surface of the carrier is formed of a calcium phosphate based compound; antiligands provided on and surrounding the surface of the carrier; a blocking layer formed of a protein having low interaction with antigens or antibodies, the blocking layer being formed on a portion of the surface of the carrier where the antiligands are not provided; and antigens or antibodies each having a portion that is irrelevant to the antigen-antibody reaction on which a ligand is provided, the antigens or antibodies being immobilized to the surface of the carrier through the bonding between the ligands and the antiligands with the blocking layer effectively preventing antigens or antibodies from being directly absorbed to the surface without bonding between the ligands and the antiligands.

Abstract: Cell culture carriers that allow cells to efficiently adhere thereto and sufficiently grow thereon and from which the grown cells can be easily removed or detached, a method for manufacturing cell culture carriers that enables to easily and reliably manufacture such cell culture carriers and a method for culturing cells using such cell culture carriers are disclosed. The cell culture carrier includes a base material having a granular shape and a coating layer that is provided so as to cover the surface of the base material, the coating layer is mainly made of calcium phosphate-based apatite in which a part of calcium is deficient. Such cell culture carriers are utilized in cell culture in which cells adhere to and grow on the surface of cell culture carriers, particularly in three-dimensional high-density culture (suspension culture). The Ca deficiency rate in the calcium phosphate-based apatite in which a part of calcium is deficient is preferably in the range of 1 to 30 mol %.

Abstract: A cell culturing method comprises the steps of preparing a cell culture solution containing at least cells to be cultured and granular cell culture carriers; and applying a magnetic field to the cell culture solution so as to agitate the cell culture solution by the effect of the magnetic field, whereby the cells adhere to and grow on the surfaces of the cell culture carriers. Each of the carriers comprises a magnetic particle having a coating layer which is provided to cover at least a part of the surface of the magnetic particle so that the cells are allowed to adhere thereto, wherein the cell culture carriers are moved in the culture solution by the application of the magnetic field, thereby agitating the culture solution. Alternatively, the culture solution further contains magnetic particles, and the magnetic particles are moved in the culture solution by the application of the magnetic field, thereby agitating the culture solution.

Abstract: A carrier for cell culture which enables cells to efficiently grow thereon is provided. A carrier 1 comprises a particulate base body 2 mainly formed of a resin material and a coating layer 3. The coating layer 3 is formed from particles 31 of a calcium phosphate-based compound, and the particles 31 are partially embedded in the base body 2 at the vicinity of the surface thereof whereby the coating layer 3 coats the surface of the base body 2 with a calcium phosphate-based compound. Such a carrier 1 enables cells to adhere to and grow on the surface thereof. Therefore, the carrier 1 can be used for cell culture, especially high-density three-dimensional cell culture. The coating layer 3 can be formed, for example, by colliding porous particles of a calcium phosphate-based compound against the surface of the base body 2.

Abstract: Disclosed herein is a carrier having immobilized antigens or antibodies which has a high bonding ability with antigens or antibodies (bonding objects) in the sample to be examined, and a method of manufacturing thereof. The carrier having immobilized antigens or antibodies includes a carrier having a surface which is formed of a calcium phosphate based compound, and antigens or antibodies each having a portion that is irrelevant to the antigen-antibody reaction, each of the antigens or antibodies being immobilized to the surface of the carrier through the irrelevant portion thereof. The surface of the carrier has a portion in which the antigens or antibodies are not immobilized, and at least a part of the portion of the surface is coated with a protein having low interaction with antigens or antibodies. The antigens or antibodies are stabilized by a stabilizing treatment.